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The map label for this gene is trpA

Identifier: 11499192

GI number: 11499192

Start: 1434885

End: 1435631

Strand: Reverse

Name: trpA

Synonym: AF1599

Alternate gene names: 11499192

Gene position: 1435631-1434885 (Counterclockwise)

Preceding gene: 11499193

Following gene: 11499191

Centisome position: 65.9

GC content: 53.82

Gene sequence:

>747_bases
ATGATTGACAGGTCGCTGATAGTTTTCTTCACGGCCTGCTATCCAACTGCTGAGAAAACTGTTGAGTTCATGCTCACTGC
CGCTGAATCCGGTGCTGACGTTATCGAGCTTGGTGTGCCCTTCAGCGACCCGGTGGCTGACGGCAAAACAATTCAGGAGT
CCTACGTCAGGGCTCTGAGGAACTTCAGGGTTGAGCGGGTTTTCGAGATTGCGAAGGCCTTCAGGGCTGAGTCGGACAAA
CCGCTCGTGCTGATGAGCTACTACAATCCAATCTACCGCAGGGGTGTGGAGAGCTTTGTGGAGAAAGCCTACTCCTCAGG
GATAGACGCCATGCTCGTTGTTGACTTGCCCTACGACGAGGCCGGAGACTTTGTGGAGGTTTGCAGCAGAACTGGGATGA
AGAACGTTTTTCTGGCTGCTCCAAACACGCCTGAAGATAGGCTGAGGGCGATGGACGAGCTTTCAGCTTTCGTTTATCTG
GTTTCGACCTACGGTGTTACGGGAGAGAGGGACAGAATCTCCCCGCTTGCCTTTGAAGCGCTGAAGAGGGCTAAGGGCAT
CTGCAGGAAGCCAGTAGCTGTCGGATTTGGGGTGTCAAAGGCCGATCATGTGAGACAGCTAATCAGCGCGGGGGCGGATG
GGGTTGTTGTTGGAAGCGCCTTTGTTCGGCTGATAAATGAGAAAGGGGAGCGTGCAACTGAGGACATAAGGGCTCTGACA
GAGAACCTGAGAAGCGGTTTGGTTTGA

Upstream 100 bases:

>100_bases
CGATGAAAATGGCTGAGGAGATGCAGAGAGACGACGTTCTGGTGGTGAACCTTTCTGGCAGAGGGGATAAGGACATGGAC
ATCGTGAGGAGGAGGTTGGC

Downstream 100 bases:

>100_bases
CTCCTTGACAGCAAGCACCAAAAATTTTTTATTTCTAATGCTATTGCACAACGTATGGTAACAAAAAGAAGTCTTTTGAA
GCTGTACCTTCTCGGAATAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIDRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALRNFRVERVFEIAKAFRAESDK
PLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDEAGDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELSAFVYL
VSTYGVTGERDRISPLAFEALKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT
ENLRSGLV

Sequences:

>Translated_248_residues
MIDRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALRNFRVERVFEIAKAFRAESDK
PLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDEAGDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELSAFVYL
VSTYGVTGERDRISPLAFEALKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT
ENLRSGLV
>Mature_248_residues
MIDRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALRNFRVERVFEIAKAFRAESDK
PLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDEAGDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELSAFVYL
VSTYGVTGERDRISPLAFEALKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT
ENLRSGLV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=238, Percent_Identity=32.3529411764706, Blast_Score=122, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=37.9746835443038, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ARCFU (O28673)

Other databases:

- EMBL:   AE000782
- PIR:   F69449
- RefSeq:   NP_070428.1
- ProteinModelPortal:   O28673
- SMR:   O28673
- GeneID:   1484825
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1599
- NMPDR:   fig|224325.1.peg.1589
- TIGR:   AF_1599
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- BioCyc:   AFUL224325:AF_1599-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27301; Mature: 27301

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 36-36 ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALR
CCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
NFRVERVFEIAKAFRAESDKPLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDE
HHHHHHHHHHHHHHHCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AGDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELSAFVYLVSTYGVTGERDRISPLAFEA
CCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT
HHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHH
ENLRSGLV
HHHHHCCC
>Mature Secondary Structure
MIDRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALR
CCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
NFRVERVFEIAKAFRAESDKPLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDE
HHHHHHHHHHHHHHHCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AGDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELSAFVYLVSTYGVTGERDRISPLAFEA
CCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT
HHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHH
ENLRSGLV
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475