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The map label for this gene is trpB-2

Identifier: 11499193

GI number: 11499193

Start: 1435628

End: 1436821

Strand: Reverse

Name: trpB-2

Synonym: AF1600

Alternate gene names: 11499193

Gene position: 1436821-1435628 (Counterclockwise)

Preceding gene: 11499194

Following gene: 11499192

Centisome position: 65.96

GC content: 54.44

Gene sequence:

>1194_bases
ATGAGATGCTGGTTAGAGAATTTGTCAGGAGGGCGAAAAATGAAGTTCGGTGAGTTTGGCGGAAGATTTGTTCCGGAGGT
TCTTATTCCACCTCTGGAGGAGCTTGAAAAAGCTTATGACAGGTTTAAGGATGATGAGGAGTTCAAAGCAAGGCTGGAAT
ACTACCTCAAAAGCTACGCGGGGAGGCCAACTCCCCTCTACTTTGCAGAGAATCTCAGCAGAGAGCTTGGAGTGAAAATT
TACCTGAAGAGGGAGGATTTGCTTCACGGCGGGGCGCACAAAATCAACAACACAATCGGCCAGGCTCTGCTGGCCAAATT
CATGGGGAAGAAGAGGGTTATCGCAGAAACCGGCGCTGGGCAGCATGGTGTGGCAACGGCAATGGCGGCAGCCCTTCTTG
GACTGGAGGCGGAGATTTACATGGGGGCTGAGGATTACGAGAGGCAGAAGATGAACGTTTTCAGGATGGAGCTGCTTGGG
GCGAAGGTTACAGCAGTAGAGAGTGGAAGCAGAACGCTGAAGGATGCGATAAATGAGGCCCTTCGGGACTGGGTTGAGAG
CTTCGAGCACACCCACTACCTCATCGGCTCGGTTGTGGGGCCACATCCTTTCCCCACCATCGTGAGGGACTTTCAGGCCG
TTATCGGGAAGGAGGCGAGGAGGCAGATTATTGAGGCTGAGGGAGGGATGCCGGACGCCATCATCGCCTGCGTTGGAGGG
GGCAGCAACGCCATGGGAATCTTCCATCCCTTCCTGAACGATGACGTTAGGCTGATAGGTGTTGAGGCTGGCGGGGAGGG
GATTGAGAGCGGCAGGCATTCAGCATCCCTTACAGCGGGAAGTAAGGGTGTGCTTCACGGCATGCTGTCCTACTTCCTGC
AGGATGAGGAGGGGATGATGCTCGACACCCACAGCGTTTCTGCCGGGCTTGACTATCCCGGAGTTGGGCCTGAGCATGCC
TACTTGAAGGAGACAGGGAGGTGTGAGTACGTAACGGTGAACGATGAGGAGGCTTTGAGAGCATTCAAGACGCTTTCAAA
GCTTGAGGGGATAATTCCCGCCCTGGAATCTGCTCACGCCATTGCCTACGCGATGAAAATGGCTGAGGAGATGCAGAGAG
ACGACGTTCTGGTGGTGAACCTTTCTGGCAGAGGGGATAAGGACATGGACATCGTGAGGAGGAGGTTGGCATGA

Upstream 100 bases:

>100_bases
GCCGGAGGGCTTAATTTGGACAACGTGAGGGAGGCGATAAGGTTCGTGAGGCCCATGGGCGTTGACGTCTCGTCCGGAGT
GGAGAGAGACGGGCTTAAGG

Downstream 100 bases:

>100_bases
TTGACAGGTCGCTGATAGTTTTCTTCACGGCCTGCTATCCAACTGCTGAGAAAACTGTTGAGTTCATGCTCACTGCCGCT
GAATCCGGTGCTGACGTTAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRCWLENLSGGRKMKFGEFGGRFVPEVLIPPLEELEKAYDRFKDDEEFKARLEYYLKSYAGRPTPLYFAENLSRELGVKI
YLKREDLLHGGAHKINNTIGQALLAKFMGKKRVIAETGAGQHGVATAMAAALLGLEAEIYMGAEDYERQKMNVFRMELLG
AKVTAVESGSRTLKDAINEALRDWVESFEHTHYLIGSVVGPHPFPTIVRDFQAVIGKEARRQIIEAEGGMPDAIIACVGG
GSNAMGIFHPFLNDDVRLIGVEAGGEGIESGRHSASLTAGSKGVLHGMLSYFLQDEEGMMLDTHSVSAGLDYPGVGPEHA
YLKETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHAIAYAMKMAEEMQRDDVLVVNLSGRGDKDMDIVRRRLA

Sequences:

>Translated_397_residues
MRCWLENLSGGRKMKFGEFGGRFVPEVLIPPLEELEKAYDRFKDDEEFKARLEYYLKSYAGRPTPLYFAENLSRELGVKI
YLKREDLLHGGAHKINNTIGQALLAKFMGKKRVIAETGAGQHGVATAMAAALLGLEAEIYMGAEDYERQKMNVFRMELLG
AKVTAVESGSRTLKDAINEALRDWVESFEHTHYLIGSVVGPHPFPTIVRDFQAVIGKEARRQIIEAEGGMPDAIIACVGG
GSNAMGIFHPFLNDDVRLIGVEAGGEGIESGRHSASLTAGSKGVLHGMLSYFLQDEEGMMLDTHSVSAGLDYPGVGPEHA
YLKETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHAIAYAMKMAEEMQRDDVLVVNLSGRGDKDMDIVRRRLA
>Mature_397_residues
MRCWLENLSGGRKMKFGEFGGRFVPEVLIPPLEELEKAYDRFKDDEEFKARLEYYLKSYAGRPTPLYFAENLSRELGVKI
YLKREDLLHGGAHKINNTIGQALLAKFMGKKRVIAETGAGQHGVATAMAAALLGLEAEIYMGAEDYERQKMNVFRMELLG
AKVTAVESGSRTLKDAINEALRDWVESFEHTHYLIGSVVGPHPFPTIVRDFQAVIGKEARRQIIEAEGGMPDAIIACVGG
GSNAMGIFHPFLNDDVRLIGVEAGGEGIESGRHSASLTAGSKGVLHGMLSYFLQDEEGMMLDTHSVSAGLDYPGVGPEHA
YLKETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHAIAYAMKMAEEMQRDDVLVVNLSGRGDKDMDIVRRRLA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=386, Percent_Identity=59.0673575129534, Blast_Score=466, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321412, Length=390, Percent_Identity=56.4102564102564, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB1_ARCFU (O28672)

Other databases:

- EMBL:   AE000782
- PIR:   G69449
- RefSeq:   NP_070429.1
- ProteinModelPortal:   O28672
- SMR:   O28672
- GeneID:   1484826
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1600
- NMPDR:   fig|224325.1.peg.1590
- TIGR:   AF_1600
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   AFUL224325:AF_1600-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43746; Mature: 43746

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCWLENLSGGRKMKFGEFGGRFVPEVLIPPLEELEKAYDRFKDDEEFKARLEYYLKSYA
CCCCCCCCCCCCEEECHHCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GRPTPLYFAENLSRELGVKIYLKREDLLHGGAHKINNTIGQALLAKFMGKKRVIAETGAG
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
QHGVATAMAAALLGLEAEIYMGAEDYERQKMNVFRMELLGAKVTAVESGSRTLKDAINEA
CCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
LRDWVESFEHTHYLIGSVVGPHPFPTIVRDFQAVIGKEARRQIIEAEGGMPDAIIACVGG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GSNAMGIFHPFLNDDVRLIGVEAGGEGIESGRHSASLTAGSKGVLHGMLSYFLQDEEGMM
CCCCCEEECCCCCCCEEEEEEECCCCHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCCCE
LDTHSVSAGLDYPGVGPEHAYLKETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHA
EECCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYAMKMAEEMQRDDVLVVNLSGRGDKDMDIVRRRLA
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRCWLENLSGGRKMKFGEFGGRFVPEVLIPPLEELEKAYDRFKDDEEFKARLEYYLKSYA
CCCCCCCCCCCCEEECHHCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GRPTPLYFAENLSRELGVKIYLKREDLLHGGAHKINNTIGQALLAKFMGKKRVIAETGAG
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
QHGVATAMAAALLGLEAEIYMGAEDYERQKMNVFRMELLGAKVTAVESGSRTLKDAINEA
CCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
LRDWVESFEHTHYLIGSVVGPHPFPTIVRDFQAVIGKEARRQIIEAEGGMPDAIIACVGG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GSNAMGIFHPFLNDDVRLIGVEAGGEGIESGRHSASLTAGSKGVLHGMLSYFLQDEEGMM
CCCCCEEECCCCCCCEEEEEEECCCCHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCCCE
LDTHSVSAGLDYPGVGPEHAYLKETGRCEYVTVNDEEALRAFKTLSKLEGIIPALESAHA
EECCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYAMKMAEEMQRDDVLVVNLSGRGDKDMDIVRRRLA
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475