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The map label for this gene is vhtG

Identifier: 11498977

GI number: 11498977

Start: 1233137

End: 1234198

Strand: Reverse

Name: vhtG

Synonym: AF1381

Alternate gene names: 11498977

Gene position: 1234198-1233137 (Counterclockwise)

Preceding gene: 11498978

Following gene: 11498976

Centisome position: 56.66

GC content: 52.73

Gene sequence:

>1062_bases
GTGATGATGAGCCTTACCCGAAGGAATTTTATGAAGCTTGTTGCGGCCATGGGTGCCTCAGCTTTTCTGAGCGCGTATAA
AGGGGACATAGTGAGAGCAGTTGAGGCAAAGAAGGATTACTGGCACATTTGCTGGCTGAACGGGGCAGCGTGCACGGGAT
GCACGGTATCCTTTGCTCAAGCGTCGAATCCGGATTTGGTGCAGATTCTAACTGAGATTATCGTTGGGACGTCTGGATTG
CCGATAGCTCTTCCCGACTACATGGAAACGATACATCCAGCCTCAGGAACACTCGCTGAAAAGCTTAAGGAGAGGTGGAA
AGCTGGAGAGAAGGGCAAGAGGATTCTTGTTGTTGAAGGGGCTGTCCAGAAACCCGGTTACTGTGTGGTAGCAGGAAAGG
ACTTCAGGGAGCACGTTAGGGAGGCCGCAGAGGTTGCTGATGCCATAGTTGCTGTCGGCCAGTGTGCAACCTTTGGAGGC
ATACCTGCGGCAAATCCAAATCCTACCGGGGCGATGGGACTTTCAGAGTTCCTGAGAGAGGAAGGTATTGCAAAGCCCGT
TATCAATCTTCCTCTCTGTCCCGTTAATGGGGAGCATGTCGTCATCATTCTTTCAGCTGTAATAATGGGGGCTAAGATTG
ACCTTGACGAGCATGGCAGGCCAAAGGCCCTTTTTGGCACAAACATGCATGACGAGCTCTGCCCCTACAGACCTCTGTAT
GACAGAGGAGTCTTCATTGAGGAGCCCGGAAACGGAGATGGATGCAGATTCAAGATTGGATGCAAGGGGCCTGTTGCCTT
TACAGACTGCCCCTCGAGAAGGTGGAACGGCAAGACCAGCTACTGCGTCGAAGCAGGGGCCCCATGCATTGCATGCTCAG
AGCCGGGATGGCCGGACAAGTTCTCACCCTTCTACGCTGAGCTCCCGTCAATCCCCAGCTTCCTCGGGTTGAATCCAACA
ACGCTTGGGGCTGGCATCTTTGGTGCAACCGCAGTGGGAATTGGCGTTCATGCGGTGAGAAGAAAACTGAGAGGTGAGAA
GGATGAGCAGGATAGTTATTGA

Upstream 100 bases:

>100_bases
AAAAAAGAATAAGAAATTAAAAATATTAACTTTTCTGCATTTTTTTTAATGGAAATCTTTTTTATTAATTGAAAATGCTA
AGGATAAAAATGGAGGGTCT

Downstream 100 bases:

>100_bases
CCCCGTAACGAGAATAGAGGGTCATCTCAGAGTTGAGCTCTCTCCAGAAAAGCTAAAAACCTCCACGGGAGAGTGGGATG
TTGTTAAGGAGGCTTACTGC

Product: F420-nonreducing hydrogenase (vhtG)

Products: NA

Alternate protein names: Hydrogenase subunit beta; Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MMMSLTRRNFMKLVAAMGASAFLSAYKGDIVRAVEAKKDYWHICWLNGAACTGCTVSFAQASNPDLVQILTEIIVGTSGL
PIALPDYMETIHPASGTLAEKLKERWKAGEKGKRILVVEGAVQKPGYCVVAGKDFREHVREAAEVADAIVAVGQCATFGG
IPAANPNPTGAMGLSEFLREEGIAKPVINLPLCPVNGEHVVIILSAVIMGAKIDLDEHGRPKALFGTNMHDELCPYRPLY
DRGVFIEEPGNGDGCRFKIGCKGPVAFTDCPSRRWNGKTSYCVEAGAPCIACSEPGWPDKFSPFYAELPSIPSFLGLNPT
TLGAGIFGATAVGIGVHAVRRKLRGEKDEQDSY

Sequences:

>Translated_353_residues
MMMSLTRRNFMKLVAAMGASAFLSAYKGDIVRAVEAKKDYWHICWLNGAACTGCTVSFAQASNPDLVQILTEIIVGTSGL
PIALPDYMETIHPASGTLAEKLKERWKAGEKGKRILVVEGAVQKPGYCVVAGKDFREHVREAAEVADAIVAVGQCATFGG
IPAANPNPTGAMGLSEFLREEGIAKPVINLPLCPVNGEHVVIILSAVIMGAKIDLDEHGRPKALFGTNMHDELCPYRPLY
DRGVFIEEPGNGDGCRFKIGCKGPVAFTDCPSRRWNGKTSYCVEAGAPCIACSEPGWPDKFSPFYAELPSIPSFLGLNPT
TLGAGIFGATAVGIGVHAVRRKLRGEKDEQDSY
>Mature_353_residues
MMMSLTRRNFMKLVAAMGASAFLSAYKGDIVRAVEAKKDYWHICWLNGAACTGCTVSFAQASNPDLVQILTEIIVGTSGL
PIALPDYMETIHPASGTLAEKLKERWKAGEKGKRILVVEGAVQKPGYCVVAGKDFREHVREAAEVADAIVAVGQCATFGG
IPAANPNPTGAMGLSEFLREEGIAKPVINLPLCPVNGEHVVIILSAVIMGAKIDLDEHGRPKALFGTNMHDELCPYRPLY
DRGVFIEEPGNGDGCRFKIGCKGPVAFTDCPSRRWNGKTSYCVEAGAPCIACSEPGWPDKFSPFYAELPSIPSFLGLNPT
TLGAGIFGATAVGIGVHAVRRKLRGEKDEQDSY

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=337, Percent_Identity=32.0474777448071, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789371, Length=301, Percent_Identity=33.8870431893688, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 37847; Mature: 37847

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMSLTRRNFMKLVAAMGASAFLSAYKGDIVRAVEAKKDYWHICWLNGAACTGCTVSFAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
ASNPDLVQILTEIIVGTSGLPIALPDYMETIHPASGTLAEKLKERWKAGEKGKRILVVEG
CCCCHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEC
AVQKPGYCVVAGKDFREHVREAAEVADAIVAVGQCATFGGIPAANPNPTGAMGLSEFLRE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EGIAKPVINLPLCPVNGEHVVIILSAVIMGAKIDLDEHGRPKALFGTNMHDELCPYRPLY
CCCCCCHHCCCCCCCCCCEEEEEEHHHHHCCEECCCCCCCCCEEECCCCCHHHCCCCCHH
DRGVFIEEPGNGDGCRFKIGCKGPVAFTDCPSRRWNGKTSYCVEAGAPCIACSEPGWPDK
HCCEEEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCC
FSPFYAELPSIPSFLGLNPTTLGAGIFGATAVGIGVHAVRRKLRGEKDEQDSY
CCHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMMSLTRRNFMKLVAAMGASAFLSAYKGDIVRAVEAKKDYWHICWLNGAACTGCTVSFAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
ASNPDLVQILTEIIVGTSGLPIALPDYMETIHPASGTLAEKLKERWKAGEKGKRILVVEG
CCCCHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEC
AVQKPGYCVVAGKDFREHVREAAEVADAIVAVGQCATFGGIPAANPNPTGAMGLSEFLRE
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
EGIAKPVINLPLCPVNGEHVVIILSAVIMGAKIDLDEHGRPKALFGTNMHDELCPYRPLY
CCCCCCHHCCCCCCCCCCEEEEEEHHHHHCCEECCCCCCCCCEEECCCCCHHHCCCCCHH
DRGVFIEEPGNGDGCRFKIGCKGPVAFTDCPSRRWNGKTSYCVEAGAPCIACSEPGWPDK
HCCEEEECCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCC
FSPFYAELPSIPSFLGLNPTTLGAGIFGATAVGIGVHAVRRKLRGEKDEQDSY
CCHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2265761 [H]