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The map label for this gene is vhtA

Identifier: 11498976

GI number: 11498976

Start: 1231456

End: 1233165

Strand: Reverse

Name: vhtA

Synonym: AF1380

Alternate gene names: 11498976

Gene position: 1233165-1231456 (Counterclockwise)

Preceding gene: 11498977

Following gene: 11498975

Centisome position: 56.61

GC content: 51.35

Gene sequence:

>1710_bases
GTGAGAAGGATGAGCAGGATAGTTATTGACCCCGTAACGAGAATAGAGGGTCATCTCAGAGTTGAGCTCTCTCCAGAAAA
GCTAAAAACCTCCACGGGAGAGTGGGATGTTGTTAAGGAGGCTTACTGCAGCGGAACCCTATTCAGGGGATGGGAGACGA
TTTTGAGAGGGAGAGACCCAAGAGATGCCTGGATTATAACTCAGAGAATTTGCGGTGTTTGTCCCGCTCCGCATGCGGAA
GCATCCATTCAGGCGATAGAGGCGGCATTTAACGTCACACCCACACCGGTGGCTGTTCTAATAAGAAACGTTCTTCACGG
AGCTTACTACATTTACGACCACATAATCCACTCTTACATTCTTCTCGGCCCCGAACTCGGGGTTGTTTGCAAGTATCCAC
CCATGGTGCCTCCGGCACTTGGAAAGGAAGGTGTCTCGAAGCTGGGAATCGGCTCAAGCTACGTTGGTGCTCTTGAGATA
CAGAGGAAGGCCAACGAAATTGTCGCAATCTGGGGAGGAAAGTTCCCCCACCACCAGAGCGAGTATCCGGGAGGTATGGC
GGTCAGGCCGACTGAGGACAGAATTGCCTCCTCTCTTGCCAGAATGTTCGAAATATGGAGGTGGGTTGCTGAAACGATGG
TCAAGGATTTCTACAACCTCCTTGAGGCCAACGAGAAGGTTGGTGAGGCGGTAAGCTCACTCCTCGACATCAACTTCAGG
GGGCTTCAGGATGTTGGGGCGTCAACAGGAAACTTCCTCAGCTACGGCCTCTTCCCCGATCATGAGAACTACGATGACTG
GCTTTCAGTGCTTGATTCACCTTCAAAGAGGAGCAGCGCTCTGATACACGCTGGAGCGTGGGATGGAAGCTCAAAGCCCC
TTGACATCTCAAAAATAGCTGAATATGTCAGGTATTCGTGGTACGACAATGAGAGCGGGCTTAATCCGAAAGATGAGGAC
GCACCTAAACCTGATAAAAACAAATCTGAAGCATACTCGTGGTTGAAGGCTCCCAGATACGACGGAAAGGTTTATGAAGT
GGGACCGCTTGCCAGAATGCTCAACACTTACGGCACAAGATGGAAGATACCCATCCGCAATCCTGTGACTGGAGAAGACC
ACGGCTACTTTGAGTATGAGGTGCTAAATCCAAAGGGGTCTGTTATTGACAGGCTCGCTGCGAGAGTCGCTCTTACAATC
TTAACTTCGCAGAAAGTCTTTGAGTGGTTGCTTGAGCTGAAATCCTACACCAACCAGAAGGTTACCAACTACAGGAATGT
GCCGAAGGAGGGCGAGGGTTTTGGCCTCTGGGATGCGCCAAGAGGAGGTTTGCTGCACTACATTAAAGTTAAAGACCACA
AAATAGCCAACTACCAGTGTGTTGTGCCAAGCACGTGGAATCTAAGCCCAAGAGACGATTTCGGGCAGCCCGGGCCAGTT
GAAACAGCATTAGAGGGTACTTGGCTGCCAAATCTGAGTGTACCTGAAATCTGCAACGCCATCTACCCCGAAAAGGACGT
TGACCTCTCCGCCTTGGGTTTGGGAGCGATGAGCTGGGGAGATGCCTTAGCTGCAGCCCTAACACCCCTCGGCCTTGCAG
AGCTGAACATTGAAAGGCTGAACGGCCAGATTTACAACACCTCTCTCACCCTGATGGTCGTCAGAAGCTTCGACCCGTGC
ATTGCCTGCGCTGTCCACGTGGTGAGGTAG

Upstream 100 bases:

>100_bases
CCCAGCTTCCTCGGGTTGAATCCAACAACGCTTGGGGCTGGCATCTTTGGTGCAACCGCAGTGGGAATTGGCGTTCATGC
GGTGAGAAGAAAACTGAGAG

Downstream 100 bases:

>100_bases
TATGGAGTGCAAGAAATGCGGAAGTGTGGCCGTTGAAAGGCACTCGAAGTTTGTGAGGAGCTGCCACTGGGGAATAGTGG
TTACTGGGATAATCACCGCC

Product: F420-nonreducing hydrogenase (vhtA)

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRRMSRIVIDPVTRIEGHLRVELSPEKLKTSTGEWDVVKEAYCSGTLFRGWETILRGRDPRDAWIITQRICGVCPAPHAE
ASIQAIEAAFNVTPTPVAVLIRNVLHGAYYIYDHIIHSYILLGPELGVVCKYPPMVPPALGKEGVSKLGIGSSYVGALEI
QRKANEIVAIWGGKFPHHQSEYPGGMAVRPTEDRIASSLARMFEIWRWVAETMVKDFYNLLEANEKVGEAVSSLLDINFR
GLQDVGASTGNFLSYGLFPDHENYDDWLSVLDSPSKRSSALIHAGAWDGSSKPLDISKIAEYVRYSWYDNESGLNPKDED
APKPDKNKSEAYSWLKAPRYDGKVYEVGPLARMLNTYGTRWKIPIRNPVTGEDHGYFEYEVLNPKGSVIDRLAARVALTI
LTSQKVFEWLLELKSYTNQKVTNYRNVPKEGEGFGLWDAPRGGLLHYIKVKDHKIANYQCVVPSTWNLSPRDDFGQPGPV
ETALEGTWLPNLSVPEICNAIYPEKDVDLSALGLGAMSWGDALAAALTPLGLAELNIERLNGQIYNTSLTLMVVRSFDPC
IACAVHVVR

Sequences:

>Translated_569_residues
MRRMSRIVIDPVTRIEGHLRVELSPEKLKTSTGEWDVVKEAYCSGTLFRGWETILRGRDPRDAWIITQRICGVCPAPHAE
ASIQAIEAAFNVTPTPVAVLIRNVLHGAYYIYDHIIHSYILLGPELGVVCKYPPMVPPALGKEGVSKLGIGSSYVGALEI
QRKANEIVAIWGGKFPHHQSEYPGGMAVRPTEDRIASSLARMFEIWRWVAETMVKDFYNLLEANEKVGEAVSSLLDINFR
GLQDVGASTGNFLSYGLFPDHENYDDWLSVLDSPSKRSSALIHAGAWDGSSKPLDISKIAEYVRYSWYDNESGLNPKDED
APKPDKNKSEAYSWLKAPRYDGKVYEVGPLARMLNTYGTRWKIPIRNPVTGEDHGYFEYEVLNPKGSVIDRLAARVALTI
LTSQKVFEWLLELKSYTNQKVTNYRNVPKEGEGFGLWDAPRGGLLHYIKVKDHKIANYQCVVPSTWNLSPRDDFGQPGPV
ETALEGTWLPNLSVPEICNAIYPEKDVDLSALGLGAMSWGDALAAALTPLGLAELNIERLNGQIYNTSLTLMVVRSFDPC
IACAVHVVR
>Mature_569_residues
MRRMSRIVIDPVTRIEGHLRVELSPEKLKTSTGEWDVVKEAYCSGTLFRGWETILRGRDPRDAWIITQRICGVCPAPHAE
ASIQAIEAAFNVTPTPVAVLIRNVLHGAYYIYDHIIHSYILLGPELGVVCKYPPMVPPALGKEGVSKLGIGSSYVGALEI
QRKANEIVAIWGGKFPHHQSEYPGGMAVRPTEDRIASSLARMFEIWRWVAETMVKDFYNLLEANEKVGEAVSSLLDINFR
GLQDVGASTGNFLSYGLFPDHENYDDWLSVLDSPSKRSSALIHAGAWDGSSKPLDISKIAEYVRYSWYDNESGLNPKDED
APKPDKNKSEAYSWLKAPRYDGKVYEVGPLARMLNTYGTRWKIPIRNPVTGEDHGYFEYEVLNPKGSVIDRLAARVALTI
LTSQKVFEWLLELKSYTNQKVTNYRNVPKEGEGFGLWDAPRGGLLHYIKVKDHKIANYQCVVPSTWNLSPRDDFGQPGPV
ETALEGTWLPNLSVPEICNAIYPEKDVDLSALGLGAMSWGDALAAALTPLGLAELNIERLNGQIYNTSLTLMVVRSFDPC
IACAVHVVR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=644, Percent_Identity=29.8136645962733, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787207, Length=650, Percent_Identity=29.2307692307692, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 63408; Mature: 63408

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMSRIVIDPVTRIEGHLRVELSPEKLKTSTGEWDVVKEAYCSGTLFRGWETILRGRDP
CCCCHHHHHCCHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCC
RDAWIITQRICGVCPAPHAEASIQAIEAAFNVTPTPVAVLIRNVLHGAYYIYDHIIHSYI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGPELGVVCKYPPMVPPALGKEGVSKLGIGSSYVGALEIQRKANEIVAIWGGKFPHHQS
HCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
EYPGGMAVRPTEDRIASSLARMFEIWRWVAETMVKDFYNLLEANEKVGEAVSSLLDINFR
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLQDVGASTGNFLSYGLFPDHENYDDWLSVLDSPSKRSSALIHAGAWDGSSKPLDISKIA
CHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHH
EYVRYSWYDNESGLNPKDEDAPKPDKNKSEAYSWLKAPRYDGKVYEVGPLARMLNTYGTR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCE
WKIPIRNPVTGEDHGYFEYEVLNPKGSVIDRLAARVALTILTSQKVFEWLLELKSYTNQK
EEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VTNYRNVPKEGEGFGLWDAPRGGLLHYIKVKDHKIANYQCVVPSTWNLSPRDDFGQPGPV
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCEEEECCCCCCCCCCCCCCCCCCC
ETALEGTWLPNLSVPEICNAIYPEKDVDLSALGLGAMSWGDALAAALTPLGLAELNIERL
CCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
NGQIYNTSLTLMVVRSFDPCIACAVHVVR
CCEEEECEEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MRRMSRIVIDPVTRIEGHLRVELSPEKLKTSTGEWDVVKEAYCSGTLFRGWETILRGRDP
CCCCHHHHHCCHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCC
RDAWIITQRICGVCPAPHAEASIQAIEAAFNVTPTPVAVLIRNVLHGAYYIYDHIIHSYI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGPELGVVCKYPPMVPPALGKEGVSKLGIGSSYVGALEIQRKANEIVAIWGGKFPHHQS
HCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
EYPGGMAVRPTEDRIASSLARMFEIWRWVAETMVKDFYNLLEANEKVGEAVSSLLDINFR
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLQDVGASTGNFLSYGLFPDHENYDDWLSVLDSPSKRSSALIHAGAWDGSSKPLDISKIA
CHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHH
EYVRYSWYDNESGLNPKDEDAPKPDKNKSEAYSWLKAPRYDGKVYEVGPLARMLNTYGTR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCE
WKIPIRNPVTGEDHGYFEYEVLNPKGSVIDRLAARVALTILTSQKVFEWLLELKSYTNQK
EEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VTNYRNVPKEGEGFGLWDAPRGGLLHYIKVKDHKIANYQCVVPSTWNLSPRDDFGQPGPV
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCEEEECCCCCCCCCCCCCCCCCCC
ETALEGTWLPNLSVPEICNAIYPEKDVDLSALGLGAMSWGDALAAALTPLGLAELNIERL
CCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
NGQIYNTSLTLMVVRSFDPCIACAVHVVR
CCEEEECEEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]