Click here to switch to the map view.

The map label for this gene is cbiP

Identifier: 11498936

GI number: 11498936

Start: 1195740

End: 1197134

Strand: Reverse

Name: cbiP

Synonym: AF1338

Alternate gene names: 11498936

Gene position: 1197134-1195740 (Counterclockwise)

Preceding gene: 11498937

Following gene: 11498933

Centisome position: 54.95

GC content: 52.19

Gene sequence:

>1395_bases
ATGCCTTCATTGATGGTAGGTGGAACCACCAGCAGCGCAGGCAAGAGTTTGCTCGCTGCTGCTTTTTGCAGAATTCTGGC
CAGAAGGGGCTATGACGTTGCCCCCTTTAAAGCTCAGAACATGAGCCTGAACTCCTTTGTCACTTCAAAGGGAAAGGAGA
TTGCCATCGCTCAGGCCTATCAGGCCTTTGCTGCGGGCATTGAGCCTGATGAAAGAATGAATCCCGTTCTTCTCAAGCCG
AAGGGAAACTTCGTGTCTCAGCTTGTTGTAATGGGGGAGGCTGTGGGTGACGTTGATTCAAGGAAATATTACGGGGTGAA
GGTTGAGTGGCTGAAGAGGGTTGTTGAAGAAGCTTATCTCAGCTTGGCCGAGGAGTATGACTTTGTTGTTATTGAGGGTG
CCGGTGGGATGGCGGAGATCAATCTCTATGAAAGGGATTTGCCCAACATTCACATAGCGAGGTTTGCGAGGCCTGACATT
TTGATTGTGGGCGATATAGACAGGGGCGGGGTTTTTGCTTCCCTCTACGGCACCTACGCACTCCTGCCAGATGATGTAAA
GCCGCTCGTTAAAGGGTTTGTGATAAACAGGCTCAGAGGGAGGGAGGATGTGCTTGAGAGCGGTATAAGGGAGCTTGAGA
GGCTGACTGGAATAAGGGTGCTGGGAGTTCTTCCCTACCTTGATTACAACTTTCCGTCGGAGGACTCGCTGAACATCGAG
GAGTGGGGGGCAGAAGGGACTGTTGGGATAGTAAGGCTGCCAAGAGTTTCGAACTTCACGGACTTTGAGCCCCTCAGGGA
GCATGCGAGGTTTCTCAGCCTCAACTCCTCGCTCAACGGTTGTGAGGTTGTGATTATTCCCGGCAGCAAGGACACCATTG
CTGATTTGAAGGCTCTGAAATCCTCAAAGCTGGGAGAGGAGATTGTGAGGAAGGCTGGAGAGATTCCGGTCATCGGGATA
TGCGGGGGATATCAGATAATGTGCAGAGAGCTCGTCGACATGGGCGTGGAGCATGGCAGAATCAGGGCTAAGGGGCTCGG
GCTTCTCGATGCGGTTACGGAGTTCAGGGAGTACAGGAAGAGGACGGTTCAGGTAGAGAAGAGAGTTAACGGCAACGCAG
TGATTCTGGACAGGATAAGGGGCGAGAAGGTTTGGGGCTACGAGATTCACAAGGGCATCACGAGGGCGAGCAATCCCATT
TTTGAGGATGACGGCTGTGCCAGCGAGGATGGCATGTGCTGGGGAACTTACCTCCACGGTCTCTTCTGGAATGAAAACGT
TCTGAGGGCTCTGGGAGGTTATCTGGGCATAAAATTCAGGCAAAAGGAGGATTGGGCTGATTTGATTGCGGATGAGGTGG
AAGGGAGGCTGGATTTGGGGGTTTTAGGGCTTTAA

Upstream 100 bases:

>100_bases
AAGATGCCCACTCCCACGAAAATTGTGGTTGAGAAGGGTCACGAACTGCTGAAGGGCAAGATTTGAGTAAAGTTTTTTTG
ATTCAAATCAACTTTTTCTG

Downstream 100 bases:

>100_bases
AACAACTACTTCAGGTTCCCAATTTTTGCAGCAAGCCTCGCCAGAACCTCCTTCCTCTTCGCCTGAACGAACTTTATTGA
GCCAACCACCAGATGTCCTC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPSLMVGGTTSSAGKSLLAAAFCRILARRGYDVAPFKAQNMSLNSFVTSKGKEIAIAQAYQAFAAGIEPDERMNPVLLKP
KGNFVSQLVVMGEAVGDVDSRKYYGVKVEWLKRVVEEAYLSLAEEYDFVVIEGAGGMAEINLYERDLPNIHIARFARPDI
LIVGDIDRGGVFASLYGTYALLPDDVKPLVKGFVINRLRGREDVLESGIRELERLTGIRVLGVLPYLDYNFPSEDSLNIE
EWGAEGTVGIVRLPRVSNFTDFEPLREHARFLSLNSSLNGCEVVIIPGSKDTIADLKALKSSKLGEEIVRKAGEIPVIGI
CGGYQIMCRELVDMGVEHGRIRAKGLGLLDAVTEFREYRKRTVQVEKRVNGNAVILDRIRGEKVWGYEIHKGITRASNPI
FEDDGCASEDGMCWGTYLHGLFWNENVLRALGGYLGIKFRQKEDWADLIADEVEGRLDLGVLGL

Sequences:

>Translated_464_residues
MPSLMVGGTTSSAGKSLLAAAFCRILARRGYDVAPFKAQNMSLNSFVTSKGKEIAIAQAYQAFAAGIEPDERMNPVLLKP
KGNFVSQLVVMGEAVGDVDSRKYYGVKVEWLKRVVEEAYLSLAEEYDFVVIEGAGGMAEINLYERDLPNIHIARFARPDI
LIVGDIDRGGVFASLYGTYALLPDDVKPLVKGFVINRLRGREDVLESGIRELERLTGIRVLGVLPYLDYNFPSEDSLNIE
EWGAEGTVGIVRLPRVSNFTDFEPLREHARFLSLNSSLNGCEVVIIPGSKDTIADLKALKSSKLGEEIVRKAGEIPVIGI
CGGYQIMCRELVDMGVEHGRIRAKGLGLLDAVTEFREYRKRTVQVEKRVNGNAVILDRIRGEKVWGYEIHKGITRASNPI
FEDDGCASEDGMCWGTYLHGLFWNENVLRALGGYLGIKFRQKEDWADLIADEVEGRLDLGVLGL
>Mature_463_residues
PSLMVGGTTSSAGKSLLAAAFCRILARRGYDVAPFKAQNMSLNSFVTSKGKEIAIAQAYQAFAAGIEPDERMNPVLLKPK
GNFVSQLVVMGEAVGDVDSRKYYGVKVEWLKRVVEEAYLSLAEEYDFVVIEGAGGMAEINLYERDLPNIHIARFARPDIL
IVGDIDRGGVFASLYGTYALLPDDVKPLVKGFVINRLRGREDVLESGIRELERLTGIRVLGVLPYLDYNFPSEDSLNIEE
WGAEGTVGIVRLPRVSNFTDFEPLREHARFLSLNSSLNGCEVVIIPGSKDTIADLKALKSSKLGEEIVRKAGEIPVIGIC
GGYQIMCRELVDMGVEHGRIRAKGLGLLDAVTEFREYRKRTVQVEKRVNGNAVILDRIRGEKVWGYEIHKGITRASNPIF
EDDGCASEDGMCWGTYLHGLFWNENVLRALGGYLGIKFRQKEDWADLIADEVEGRLDLGVLGL

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_ARCFU (O28931)

Other databases:

- EMBL:   AE000782
- PIR:   A69417
- RefSeq:   NP_070167.1
- ProteinModelPortal:   O28931
- GeneID:   1484564
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1338
- NMPDR:   fig|224325.1.peg.1328
- TIGR:   AF_1338
- HOGENOM:   HBG302271
- OMA:   DAISGTR
- ProtClustDB:   PRK00784
- BioCyc:   AFUL224325:AF_1338-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51313; Mature: 51181

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 321-321 ACT_SITE 419-419

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLMVGGTTSSAGKSLLAAAFCRILARRGYDVAPFKAQNMSLNSFVTSKGKEIAIAQAY
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEHHHHHH
QAFAAGIEPDERMNPVLLKPKGNFVSQLVVMGEAVGDVDSRKYYGVKVEWLKRVVEEAYL
HHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCEEECEEHHHHHHHHHHHHH
SLAEEYDFVVIEGAGGMAEINLYERDLPNIHIARFARPDILIVGDIDRGGVFASLYGTYA
HHHHCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEHEECCCCE
LLPDDVKPLVKGFVINRLRGREDVLESGIRELERLTGIRVLGVLPYLDYNFPSEDSLNIE
ECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCCCCHH
EWGAEGTVGIVRLPRVSNFTDFEPLREHARFLSLNSSLNGCEVVIIPGSKDTIADLKALK
HCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCEEEEEECCCCCHHHHHHHHH
SSKLGEEIVRKAGEIPVIGICGGYQIMCRELVDMGVEHGRIRAKGLGLLDAVTEFREYRK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
RTVQVEKRVNGNAVILDRIRGEKVWGYEIHKGITRASNPIFEDDGCASEDGMCWGTYLHG
HHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
LFWNENVLRALGGYLGIKFRQKEDWADLIADEVEGRLDLGVLGL
HHCCCHHHHHHHHHHCEEEECCHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
PSLMVGGTTSSAGKSLLAAAFCRILARRGYDVAPFKAQNMSLNSFVTSKGKEIAIAQAY
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEHHHHHH
QAFAAGIEPDERMNPVLLKPKGNFVSQLVVMGEAVGDVDSRKYYGVKVEWLKRVVEEAYL
HHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCEEECEEHHHHHHHHHHHHH
SLAEEYDFVVIEGAGGMAEINLYERDLPNIHIARFARPDILIVGDIDRGGVFASLYGTYA
HHHHCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCEEHEECCCCE
LLPDDVKPLVKGFVINRLRGREDVLESGIRELERLTGIRVLGVLPYLDYNFPSEDSLNIE
ECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCCCCHH
EWGAEGTVGIVRLPRVSNFTDFEPLREHARFLSLNSSLNGCEVVIIPGSKDTIADLKALK
HCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCEEEEEECCCCCHHHHHHHHH
SSKLGEEIVRKAGEIPVIGICGGYQIMCRELVDMGVEHGRIRAKGLGLLDAVTEFREYRK
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
RTVQVEKRVNGNAVILDRIRGEKVWGYEIHKGITRASNPIFEDDGCASEDGMCWGTYLHG
HHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
LFWNENVLRALGGYLGIKFRQKEDWADLIADEVEGRLDLGVLGL
HHCCCHHHHHHHHHHCEEEECCHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475