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The map label for this gene is alkK-4

Identifier: 11498860

GI number: 11498860

Start: 1123872

End: 1124573

Strand: Reverse

Name: alkK-4

Synonym: AF1261

Alternate gene names: 11498860

Gene position: 1124573-1123872 (Counterclockwise)

Preceding gene: 11498861

Following gene: 11498859

Centisome position: 51.62

GC content: 48.15

Gene sequence:

>702_bases
ATGCTGAAAGGAATACCTTCCACCATGAATGATGACTACCAGCTAAATGTAACAAGGATAATACAGCATGCTGCCAGAAT
CCACCCCAAAAGGGAGATTGCGTCCAGAACCTTTGCAGGGATGTTCAGATACAACTACGCCAAAGCCTACGAGCGAATTT
GTGCTATAGCAAATGCTCTTGAGGAGCTTTGGATTAATGAGCTGCCGTGGGATGGAAAGTCCGTTGGAGAGCTTTGGATC
AGGGGTTTGTGGGTTGCGAAGGAGTACCACAATGACGAGAGAACGATGAACAGCTTCGTTGACGGATGGTGGAAGAGCGG
AGATGTGGCAGTAATAACCCCTGAAGGTTATGTAAAGGTGGTTGACAGGGTTAAGGATGTGATAAAGAGCGGGGGAGAGT
GGATAAGCAGCGTTGATTTGGAGAACTATCTGATGGGACATCCAGCAGTTTTGGAAGCATGTGTTGTCGCTGCTGAGCAT
CCGAAGTGGCAGGAGAGGCCGATAGCGATTGTCGTTCCCAAGCCCGGCAGCGAAGTTACCAAGGATGAGCTGAGAGAGTT
TCTGGCGAAAAGATTTGCGAAGTGGCAGCTTCCAGACGACATAATCTTCGTTAACGAAATCCCAAAGACGAGCGTTGGAA
AGTTCGACAAGAAGAGGCTCAGGGAGCAGTATCGCAGATACCTTATAGAAAAAATGGAGTAA

Upstream 100 bases:

>100_bases
TTTCAGCAGATTATAACTGAAAATAAACTTTCAGGATTCTTAAGTAAATTAATTCGAAAAATTATTATGTCGATTAAACT
TGATTGATTGGTGAAAAACT

Downstream 100 bases:

>100_bases
AATTAAAGATCTGCCAGCTTTGAAAGTTTAGGTTTTTCTTTTTTTACTTTTCCCTAGACAGGACGATCTTAGAGAAAGGA
ATCAGACCAGCCCCACCAGC

Product: medium-chain acyl-CoA ligase (alkK-4)

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLKGIPSTMNDDYQLNVTRIIQHAARIHPKREIASRTFAGMFRYNYAKAYERICAIANALEELWINELPWDGKSVGELWI
RGLWVAKEYHNDERTMNSFVDGWWKSGDVAVITPEGYVKVVDRVKDVIKSGGEWISSVDLENYLMGHPAVLEACVVAAEH
PKWQERPIAIVVPKPGSEVTKDELREFLAKRFAKWQLPDDIIFVNEIPKTSVGKFDKKRLREQYRRYLIEKME

Sequences:

>Translated_233_residues
MLKGIPSTMNDDYQLNVTRIIQHAARIHPKREIASRTFAGMFRYNYAKAYERICAIANALEELWINELPWDGKSVGELWI
RGLWVAKEYHNDERTMNSFVDGWWKSGDVAVITPEGYVKVVDRVKDVIKSGGEWISSVDLENYLMGHPAVLEACVVAAEH
PKWQERPIAIVVPKPGSEVTKDELREFLAKRFAKWQLPDDIIFVNEIPKTSVGKFDKKRLREQYRRYLIEKME
>Mature_233_residues
MLKGIPSTMNDDYQLNVTRIIQHAARIHPKREIASRTFAGMFRYNYAKAYERICAIANALEELWINELPWDGKSVGELWI
RGLWVAKEYHNDERTMNSFVDGWWKSGDVAVITPEGYVKVVDRVKDVIKSGGEWISSVDLENYLMGHPAVLEACVVAAEH
PKWQERPIAIVVPKPGSEVTKDELREFLAKRFAKWQLPDDIIFVNEIPKTSVGKFDKKRLREQYRRYLIEKME

Specific function: Activates The Carboxylate Group Of 2,3-Dihydroxy- Benzoate (2,3-Dhb), Via ATP-Dependent Ppi Exchange Reactions, To The Acyladenylate. Then, Catalyzes The Acylation Of Holo-Entb With 2,3-Dhb Adenylate, Preparing That Molecule For Amide Bond Formation With

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=155, Percent_Identity=36.7741935483871, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI187761345, Length=199, Percent_Identity=30.6532663316583, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI187761343, Length=199, Percent_Identity=30.6532663316583, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI28416953, Length=159, Percent_Identity=32.0754716981132, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI38505220, Length=138, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI115511026, Length=142, Percent_Identity=31.6901408450704, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI157311624, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI157311622, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI58082049, Length=138, Percent_Identity=31.1594202898551, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI42544132, Length=138, Percent_Identity=31.8840579710145, Blast_Score=67, Evalue=9e-12,
Organism=Homo sapiens, GI122937307, Length=145, Percent_Identity=31.7241379310345, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=169, Percent_Identity=37.2781065088757, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI145693145, Length=159, Percent_Identity=36.4779874213836, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788107, Length=161, Percent_Identity=31.6770186335404, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI221142682, Length=158, Percent_Identity=30.379746835443, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790505, Length=163, Percent_Identity=27.6073619631902, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=158, Percent_Identity=36.0759493670886, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=208, Percent_Identity=28.8461538461538, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=150, Percent_Identity=30.6666666666667, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=153, Percent_Identity=28.7581699346405, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=143, Percent_Identity=33.5664335664336, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=149, Percent_Identity=32.2147651006711, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=149, Percent_Identity=31.5436241610738, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=186, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994694, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996755, Length=147, Percent_Identity=34.0136054421769, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560308, Length=194, Percent_Identity=26.2886597938144, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=164, Percent_Identity=32.3170731707317, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323903, Length=104, Percent_Identity=40.3846153846154, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322182, Length=104, Percent_Identity=39.4230769230769, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324893, Length=101, Percent_Identity=35.6435643564356, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18859661, Length=168, Percent_Identity=35.7142857142857, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581924, Length=155, Percent_Identity=30.9677419354839, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=153, Percent_Identity=28.7581699346405, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648260, Length=135, Percent_Identity=31.1111111111111, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=156, Percent_Identity=27.5641025641026, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24667955, Length=140, Percent_Identity=27.8571428571429, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI62472339, Length=140, Percent_Identity=27.8571428571429, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24667959, Length=140, Percent_Identity=27.8571428571429, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 27066; Mature: 27066

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGIPSTMNDDYQLNVTRIIQHAARIHPKREIASRTFAGMFRYNYAKAYERICAIANAL
CCCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELWINELPWDGKSVGELWIRGLWVAKEYHNDERTMNSFVDGWWKSGDVAVITPEGYVKV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHHH
VDRVKDVIKSGGEWISSVDLENYLMGHPAVLEACVVAAEHPKWQERPIAIVVPKPGSEVT
HHHHHHHHHCCCHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
KDELREFLAKRFAKWQLPDDIIFVNEIPKTSVGKFDKKRLREQYRRYLIEKME
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKGIPSTMNDDYQLNVTRIIQHAARIHPKREIASRTFAGMFRYNYAKAYERICAIANAL
CCCCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELWINELPWDGKSVGELWIRGLWVAKEYHNDERTMNSFVDGWWKSGDVAVITPEGYVKV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHHH
VDRVKDVIKSGGEWISSVDLENYLMGHPAVLEACVVAAEHPKWQERPIAIVVPKPGSEVT
HHHHHHHHHCCCHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
KDELREFLAKRFAKWQLPDDIIFVNEIPKTSVGKFDKKRLREQYRRYLIEKME
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]