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The map label for this gene is noxB-2

Identifier: 11498861

GI number: 11498861

Start: 1124707

End: 1126605

Strand: Reverse

Name: noxB-2

Synonym: AF1262

Alternate gene names: 11498861

Gene position: 1126605-1124707 (Counterclockwise)

Preceding gene: 11498862

Following gene: 11498860

Centisome position: 51.72

GC content: 49.76

Gene sequence:

>1899_bases
ATGAAATTATTTGAGCCAATCGAGTTTGGAGGAATGAAGGTAAAGAACAGAATAGTTCTCCCAGCAGTGGGACTGAACTA
CACAACGCCAGATGGAAGGGTTACAGATAAGCTGCTCAGGTTCTACGAAGAGAGGGCGAAGGGAGGGATAGGTTTTGCGG
TTGTAGGGATTGCCAAAATCGAACCCCACTTCTTTGGTGGAATAGCAGCACACTCCGACGAATTTATTCCAGACCTCAAG
AAGATAGCCGACGTCTTCCACAAGTACGACGTGAAATGCGCTTTACAGCTATGGCACCCAGGCAGATACGAAATCTCCTT
CGACCCCAACCGCCAACCAGTCGCCCCATCCCCCATACCACCACCAATCTTCACCAAGCGCACTCCCAAAGAGCTCACCA
AAGAAGAAATCCTTCAAATCGAGGAGGAGTTTGCTGATGCAGCTGTGAGGGCGAAGAAAGCTGGCTTCGATGCTGTCGAG
CTTATTGGTTCAGCAGGCTACCTCATTTCCCAGTTCTTCAGCCCCGCAACGAACAAAAGAACGGACGAGTATGGGGGCAG
CTTGGAAAACCGCACGAGGTTTGCGGTTGAGATAATTCAAAAAATCAAGGAAAAGTGCGGAGAGAGCTATCCAGTAATGA
TACGAATCCCCGGAGACGAGTTCATCGAGGGAGGGAACACAGTCAGAGAAATGAAGCAGATTGCCAAAATATTGGAGGAT
GCAGGAGTTGTAGCAATCAACGTAATGGCGGGCTGGCACGAGTCCAGAAAGCCTTTGACAACAATGCTCGTTCCCAGAGG
AGGTTTTGCTTACCTTGCAGCAGAGATAAAGAGCGCTGTAAGCGTTTCCGTTATCGCATCTCACCGCATCAATGATCCGA
TTGTGGCTGAAAAGATACTGCAGGAGGGCAAGGCAGATATGGTTGCAATGCTGAGAGCTTTGATTGCCGACCCTGAACTG
CCGAAGAAGGCCAAGGAGGGAAGGTTTGATGAGATAAGGTATTGCGTGGCCTGCAACCAGGGCTGCATGGACATGGTTAT
GCAGGCTCAGCCAGTTACATGCCTCGTTAACCCCATAGTTGGAAGGGAGGCTGAGTTTGAAAATCTGAAAGCAGAAAAAC
CAAAGAAGGTTGTTATTGTCGGAGGTGGACCGGGAGGATGCATGGCTGCAGAGATGCTTGCAAGAAAGGGGCATAAGGTA
GTTTTATTCGAGAAAACTGACAAATTGGGAGGGCAGCTCAACTTGGCTGCGAAGTCACCTTTGGGCTATGAATTTGCAGA
AGTGGGCAAGTACTTCATGAACGTCCTGCCAAAGCTTGGGGTAGAGGTGAGATACAACACGGAGGCTGATGCGGGCAAAG
TTTTAGCAGAGAACCCTGATGTTGCGGTAATAGCCGTTGGAGCTTCTCCTTTGATCCCACCAATTCCGGGAGTTGAGAAT
GCTGTAACAGCCTTCGATGTCCTCGCAGGAAGGGCAGAGGTTGGAAACAGTGTGGTGGTTATAGGCGGAGGAGGAGTTGG
ATGCGATGTTGCAGCAGAGCTTGCAAATGAGGGGAAGAAGGTTACAATTGTGGAGATGTTGCCGAAGATTGGCCAGGATA
TAGGCATCTCGACAAGATGGACCGTATTGATGTACCTGAAAGAGAAGGGAGTGGAGATGCTCACCAACACGAAGGCTGTT
GAAATCAAGCCCAATGCAGTGGTTGTGGAGCAGAATGGTGAGAGGAAAGAGTTGCAGTGTGACACTGCTGTTATAGCTGT
CGGTACAAAGCCGAACAATGGGCTGTATGACGAGCTTCAGGGCAAGGTTAGCGAGCTCTACAAGATTGGAGACTGCGTTA
AGCCCAGAAAGGCTCTCGATGCTACAAGGGAGGGAGCTGAGCTGGCTTTGAAGGTTTAA

Upstream 100 bases:

>100_bases
GAGGGAGGAGTTGCAGGAGTGGCCCGACCACCTGACCTACTATCAGGAGTTGCTGGCAGACAGGGGGAAGGACCCAGCAG
GTTTGTTCTGAGGTGATGGG

Downstream 100 bases:

>100_bases
TACATCGTAAGTTCATTTTCCAACTTTTTTGTTTTTCAGCAGATTATAACTGAAAATAAACTTTCAGGATTCTTAAGTAA
ATTAATTCGAAAAATTATTA

Product: NADH oxidase (noxB-2)

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MKLFEPIEFGGMKVKNRIVLPAVGLNYTTPDGRVTDKLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVSVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGAELALKV

Sequences:

>Translated_632_residues
MKLFEPIEFGGMKVKNRIVLPAVGLNYTTPDGRVTDKLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVSVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGAELALKV
>Mature_632_residues
MKLFEPIEFGGMKVKNRIVLPAVGLNYTTPDGRVTDKLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVSVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGAELALKV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=681, Percent_Identity=35.0954478707783, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787939, Length=346, Percent_Identity=31.5028901734104, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=354, Percent_Identity=29.3785310734463, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=27.8551532033426, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=347, Percent_Identity=26.8011527377522, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=331, Percent_Identity=27.7945619335347, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=207, Percent_Identity=32.8502415458937, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=26.3888888888889, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=273, Percent_Identity=25.2747252747253, Blast_Score=83, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=222, Percent_Identity=30.1801801801802, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28574881, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665539, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665547, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665543, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 68587; Mature: 68587

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFEPIEFGGMKVKNRIVLPAVGLNYTTPDGRVTDKLLRFYEERAKGGIGFAVVGIAKI
CCCCCCCCCCCEEECCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
EPHFFGGIAAHSDEFIPDLKKIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIP
CCHHCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
PPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVELIGSAGYLISQFFSPATNKR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCC
TDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHC
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVSVIASHRINDPIVAEKIL
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEHHHCCCCCHHHHHHHH
QEGKADMVAMLRALIADPELPKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIV
HCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEEECCCCCHHHHHHCCCCCEEEECCCC
GREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKVVLFEKTDKLGGQLNLAAKSP
CCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCC
LGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCH
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRW
HHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHH
TVLMYLKEKGVEMLTNTKAVEIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQ
EEEEEEHHCCHHHHCCCEEEEECCCEEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHH
GKVSELYKIGDCVKPRKALDATREGAELALKV
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKLFEPIEFGGMKVKNRIVLPAVGLNYTTPDGRVTDKLLRFYEERAKGGIGFAVVGIAKI
CCCCCCCCCCCEEECCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
EPHFFGGIAAHSDEFIPDLKKIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIP
CCHHCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
PPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVELIGSAGYLISQFFSPATNKR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCC
TDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHC
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVSVIASHRINDPIVAEKIL
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEHHHCCCCCHHHHHHHH
QEGKADMVAMLRALIADPELPKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIV
HCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEEECCCCCHHHHHHCCCCCEEEECCCC
GREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKVVLFEKTDKLGGQLNLAAKSP
CCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCC
LGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCH
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRW
HHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHH
TVLMYLKEKGVEMLTNTKAVEIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQ
EEEEEEHHCCHHHHCCCEEEEECCCEEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHH
GKVSELYKIGDCVKPRKALDATREGAELALKV
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]