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The map label for this gene is argF

Identifier: 11498854

GI number: 11498854

Start: 1119400

End: 1120323

Strand: Reverse

Name: argF

Synonym: AF1255

Alternate gene names: 11498854

Gene position: 1120323-1119400 (Counterclockwise)

Preceding gene: 11498855

Following gene: 18677784

Centisome position: 51.43

GC content: 50.97

Gene sequence:

>924_bases
ATGAAGCATCTTCTTTCGATGGCTGACCTCGAAAAGGATGAGCTTTTCGAGATACTCAAGCTTGCCGAGAAGCTAAAGGA
GGAAAGGTACAAGGGCGTTGTTACCGATTACCTGAAAAACAAAAGCCTCGCAATGATCTTCGAACTTCCTTCAACGAGAA
CAAGAGTATCTTTTGAGGTTGCTATGACCGACTTGGGCGGCCACGCGCTCTACCTCGGGTGGGACGAACTTCAGCTCGGC
AGAGGGGAGCCGATAAAGGACACAGCGAGAGTTCTCAGCAGATACGTTCATGCGGTAATGATGAGGGTTAGGGAGCACAG
CACCATCGAGGAGTTTGCCAGATACTCAACAGTTCCAGTTATCAACGGTCTGAGCAACCTTGAGCATCCGTGCCAGGTTA
TTGCGGATTTGCTGACGATTTACGAGTACAGAGGGGACTTCAAGGACGTCACTCTCGCATGGGTTGGGGATGGAAACAAC
GTCTGCAACTCCATGATTCTCGCCGCTGCGCTAACCGGAATGAAGATGGTCATCTCAACACCTGAAAACTACGACCCAAA
TCCCGAGATTGTTAAGAAAGCCGAGGAAATGGGTGCAAAGCTGCGGTTCGTGAGAGACCCGAAGGAGGCTGTTAAGGAGG
CGGATGTGATTTACACCGACGTCTGGACGTCGATGGGTCAGGAGGCCGAGAAGGAGGCTAGGCTAAAGGCGTTTCAGCCG
TATCAGGTTTCAGATGAGCTGCTCAAGTTCTCAAAGGATGATGTCGTCGTTATGCACTGCTTACCAGCACACAGAGGGGA
GGAGATTACGGATGAGGTGATGGAAGGTAAGCACTCCATCGTGTTTGATCAGGCTGAAAACAGATTGCACGCCCAGAAGG
CAATACTGCTTAAGCTGCTCGGGAAGGAGAGCGAGGTTTACTGA

Upstream 100 bases:

>100_bases
CATCGAGAAACCATTCACTAGAAAGTACCTCTTGGACAGGATAGAGCACTACCTCCAAAAACAGAAAGTTTAATTAATTA
ACCGGAAATTTTTAACCGGT

Downstream 100 bases:

>100_bases
GTTAATTGAAAGTGTGGTTCTACACAAAAAATGGAATTTCTTCTATTTTCTTTAAAGTGAGATTAAAAACCAATTATTCA
CTCGTTCTGCTGCTCCGTCA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKHLLSMADLEKDELFEILKLAEKLKEERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEVAMTDLGGHALYLGWDELQLG
RGEPIKDTARVLSRYVHAVMMRVREHSTIEEFARYSTVPVINGLSNLEHPCQVIADLLTIYEYRGDFKDVTLAWVGDGNN
VCNSMILAAALTGMKMVISTPENYDPNPEIVKKAEEMGAKLRFVRDPKEAVKEADVIYTDVWTSMGQEAEKEARLKAFQP
YQVSDELLKFSKDDVVVMHCLPAHRGEEITDEVMEGKHSIVFDQAENRLHAQKAILLKLLGKESEVY

Sequences:

>Translated_307_residues
MKHLLSMADLEKDELFEILKLAEKLKEERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEVAMTDLGGHALYLGWDELQLG
RGEPIKDTARVLSRYVHAVMMRVREHSTIEEFARYSTVPVINGLSNLEHPCQVIADLLTIYEYRGDFKDVTLAWVGDGNN
VCNSMILAAALTGMKMVISTPENYDPNPEIVKKAEEMGAKLRFVRDPKEAVKEADVIYTDVWTSMGQEAEKEARLKAFQP
YQVSDELLKFSKDDVVVMHCLPAHRGEEITDEVMEGKHSIVFDQAENRLHAQKAILLKLLGKESEVY
>Mature_307_residues
MKHLLSMADLEKDELFEILKLAEKLKEERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEVAMTDLGGHALYLGWDELQLG
RGEPIKDTARVLSRYVHAVMMRVREHSTIEEFARYSTVPVINGLSNLEHPCQVIADLLTIYEYRGDFKDVTLAWVGDGNN
VCNSMILAAALTGMKMVISTPENYDPNPEIVKKAEEMGAKLRFVRDPKEAVKEADVIYTDVWTSMGQEAEKEARLKAFQP
YQVSDELLKFSKDDVVVMHCLPAHRGEEITDEVMEGKHSIVFDQAENRLHAQKAILLKLLGKESEVY

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=42.4342105263158, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=41.4634146341463, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1786469, Length=328, Percent_Identity=41.1585365853659, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=31.5112540192926, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI48994908, Length=360, Percent_Identity=24.1666666666667, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=325, Percent_Identity=29.5384615384615, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=37.741935483871, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=30.6188925081433, Blast_Score=115, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=29.5819935691318, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_ARCFU (O29013)

Other databases:

- EMBL:   AE000782
- PIR:   F69406
- RefSeq:   NP_070083.1
- ProteinModelPortal:   O29013
- SMR:   O29013
- GeneID:   1484479
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1255
- NMPDR:   fig|224325.1.peg.1243
- TIGR:   AF_1255
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- ProtClustDB:   PRK00779
- BioCyc:   AFUL224325:AF_1255-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34914; Mature: 34914

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 102-102 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSMADLEKDELFEILKLAEKLKEERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEE
AMTDLGGHALYLGWDELQLGRGEPIKDTARVLSRYVHAVMMRVREHSTIEEFARYSTVPV
EEECCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
INGLSNLEHPCQVIADLLTIYEYRGDFKDVTLAWVGDGNNVCNSMILAAALTGMKMVIST
HHCHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEC
PENYDPNPEIVKKAEEMGAKLRFVRDPKEAVKEADVIYTDVWTSMGQEAEKEARLKAFQP
CCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
YQVSDELLKFSKDDVVVMHCLPAHRGEEITDEVMEGKHSIVFDQAENRLHAQKAILLKLL
CCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
GKESEVY
CCCCCCC
>Mature Secondary Structure
MKHLLSMADLEKDELFEILKLAEKLKEERYKGVVTDYLKNKSLAMIFELPSTRTRVSFEV
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEE
AMTDLGGHALYLGWDELQLGRGEPIKDTARVLSRYVHAVMMRVREHSTIEEFARYSTVPV
EEECCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
INGLSNLEHPCQVIADLLTIYEYRGDFKDVTLAWVGDGNNVCNSMILAAALTGMKMVIST
HHCHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEC
PENYDPNPEIVKKAEEMGAKLRFVRDPKEAVKEADVIYTDVWTSMGQEAEKEARLKAFQP
CCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
YQVSDELLKFSKDDVVVMHCLPAHRGEEITDEVMEGKHSIVFDQAENRLHAQKAILLKLL
CCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
GKESEVY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475