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The map label for this gene is oadA

Identifier: 18677784

GI number: 18677784

Start: 1116935

End: 1118377

Strand: Reverse

Name: oadA

Synonym: AF1252m

Alternate gene names: 18677784

Gene position: 1118377-1116935 (Counterclockwise)

Preceding gene: 11498854

Following gene: 11498850

Centisome position: 51.34

GC content: 51.84

Gene sequence:

>1443_bases
ATGGCAAAAGTAAAAATTGTTGACTTAACTCTTAGAGACGGACACCAGTCCCTGCTTGCCACAAGAATGAGAACGAGAGA
CATGCTCCCAATTCTCGAAACTCTCGACGAGTCGGGAATTTACTCGCTTGAAGTTTGGGGCGGTGCAACCTTTGACGCCT
GCCACCGCTTTCTAAACGAGAACCCGTGGGAGAGGCTGAGAGAAATCAGAAAGAGAGTTAAGAACGCAAAGCTTCAGATG
CTGCTTCGAGGGCAAAATCTCGTGGGATACCGCCACTATCCTGACGATATTGTTGAGAAGTTTGTCCAGAAAACGATAGA
AAACGGACTCGACGTTTTCAGAATTTTCGACGCTCTTAACGACGTAAGGAATTTGATTTCGTCCATTAAGGCTGCTAAAA
AGTACAATGCGGACCACATACAGGGGACGATATGCTACACCATCTCTCCCGTCCACACCGTGGAGAAGTACGTAGAAATT
GCAAACGAGCTTGCGGCGCTTGAGGTCGATTCCATCTGCCTGAAGGATATGGCGGGGATGCTTTCTCCGAAAATGGCATA
CGAGCTTGTAAAAGCGCTGAAGAAGGAGGTTGGACTGCCAATAAACGTCCACTCACACTACACGAGCGGGATGGCTTCGA
TGGCGTTGCTGAAGGGCGTTGAGGCGGGAGCGGAAATGATTGATACCTGCATGTCTCCGCTGAGCAGCGGGACCTCCCAC
CCTCCGACGGAGAGCATGGTCTATGCTCTGAACGAGCTTGGTTACGACACAGGCGTGAAGCTGGATATTCTGCTTGAGGC
GAGAGAGTACTTCATGAAGATAAGGGAGAAGTACTCAGGCTACCTTAACCCTCTCTCGACAATTCCTGACACGAGAGTCT
TGGTTTACCAGATTCCGGGAGGGATGTTCAGCAACCTTATTGCCCAGCTTCAGGAGCAGAACGCCCTCGACAGGCTTCAG
GAGGTTCTAGAGGAGGTGCCAAGAGTGAGGGAAGACCTGGGCTATCCGCCGCTCGTCACTCCAACGAGCCAGATTGTAGG
CGTGCAGGCGGTGATTAATGTCCTGGTGGGGGAGAGGTACAAGGTCGTGACGAGGGAAACCAAGGACTTGGTGAAGGGGA
TGTACGGGAGAACTCCCGCTCCGATCAAGCCCGAGATTGTAAAGAAGATTCTTGGCGATGAAAAGCCGATCGACTGCAGA
CCTGCCGACCTGCTTGAGCCTGAGTTCGAGAAGAGGAAGCAGGAACTCATTGAGGCAGGAATAGAGAATCCCAGCGATGA
GGACGTTTTGCTCTACGCACTCTTCCCGCAAACCGGCCTGAAATTCCTGAAAGGGGAAATGGTCGAGGAGCCGTTCCCAG
TTGCGGGAGGAAAGATTGAGGGGAGCTTCGAGGTTGAGATAGACGGGCAGAAGTACGTGGTTAAAGTCGCGCCAGAAAAG
TGA

Upstream 100 bases:

>100_bases
AACCTAAATAAACTTTCCTGAAGAACGTAAATCTTGATCTTTTGAGGTGCAAGCAATATTAAAAACAAAAAATTTTTGTA
TTTCCCATCAGGCTTGAGAC

Downstream 100 bases:

>100_bases
TATACCTCTTTTTCCAATTTTTAGCATGGAGCTTGAAACGGAAATTATTGTCGACAAGGTGGATGTTGAAAACCCGATTC
TGATAACGAGCTTTCCGGGA

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAKVKIVDLTLRDGHQSLLATRMRTRDMLPILETLDESGIYSLEVWGGATFDACHRFLNENPWERLREIRKRVKNAKLQM
LLRGQNLVGYRHYPDDIVEKFVQKTIENGLDVFRIFDALNDVRNLISSIKAAKKYNADHIQGTICYTISPVHTVEKYVEI
ANELAALEVDSICLKDMAGMLSPKMAYELVKALKKEVGLPINVHSHYTSGMASMALLKGVEAGAEMIDTCMSPLSSGTSH
PPTESMVYALNELGYDTGVKLDILLEAREYFMKIREKYSGYLNPLSTIPDTRVLVYQIPGGMFSNLIAQLQEQNALDRLQ
EVLEEVPRVREDLGYPPLVTPTSQIVGVQAVINVLVGERYKVVTRETKDLVKGMYGRTPAPIKPEIVKKILGDEKPIDCR
PADLLEPEFEKRKQELIEAGIENPSDEDVLLYALFPQTGLKFLKGEMVEEPFPVAGGKIEGSFEVEIDGQKYVVKVAPEK

Sequences:

>Translated_480_residues
MAKVKIVDLTLRDGHQSLLATRMRTRDMLPILETLDESGIYSLEVWGGATFDACHRFLNENPWERLREIRKRVKNAKLQM
LLRGQNLVGYRHYPDDIVEKFVQKTIENGLDVFRIFDALNDVRNLISSIKAAKKYNADHIQGTICYTISPVHTVEKYVEI
ANELAALEVDSICLKDMAGMLSPKMAYELVKALKKEVGLPINVHSHYTSGMASMALLKGVEAGAEMIDTCMSPLSSGTSH
PPTESMVYALNELGYDTGVKLDILLEAREYFMKIREKYSGYLNPLSTIPDTRVLVYQIPGGMFSNLIAQLQEQNALDRLQ
EVLEEVPRVREDLGYPPLVTPTSQIVGVQAVINVLVGERYKVVTRETKDLVKGMYGRTPAPIKPEIVKKILGDEKPIDCR
PADLLEPEFEKRKQELIEAGIENPSDEDVLLYALFPQTGLKFLKGEMVEEPFPVAGGKIEGSFEVEIDGQKYVVKVAPEK
>Mature_479_residues
AKVKIVDLTLRDGHQSLLATRMRTRDMLPILETLDESGIYSLEVWGGATFDACHRFLNENPWERLREIRKRVKNAKLQML
LRGQNLVGYRHYPDDIVEKFVQKTIENGLDVFRIFDALNDVRNLISSIKAAKKYNADHIQGTICYTISPVHTVEKYVEIA
NELAALEVDSICLKDMAGMLSPKMAYELVKALKKEVGLPINVHSHYTSGMASMALLKGVEAGAEMIDTCMSPLSSGTSHP
PTESMVYALNELGYDTGVKLDILLEAREYFMKIREKYSGYLNPLSTIPDTRVLVYQIPGGMFSNLIAQLQEQNALDRLQE
VLEEVPRVREDLGYPPLVTPTSQIVGVQAVINVLVGERYKVVTRETKDLVKGMYGRTPAPIKPEIVKKILGDEKPIDCRP
ADLLEPEFEKRKQELIEAGIENPSDEDVLLYALFPQTGLKFLKGEMVEEPFPVAGGKIEGSFEVEIDGQKYVVKVAPEK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=498, Percent_Identity=33.5341365461847, Blast_Score=245, Evalue=7e-65,
Organism=Homo sapiens, GI106049295, Length=498, Percent_Identity=33.5341365461847, Blast_Score=245, Evalue=7e-65,
Organism=Homo sapiens, GI106049292, Length=498, Percent_Identity=33.5341365461847, Blast_Score=245, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17562816, Length=457, Percent_Identity=35.4485776805252, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6319695, Length=461, Percent_Identity=34.056399132321, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321376, Length=461, Percent_Identity=34.2733188720174, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24652212, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652210, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652214, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921944, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652216, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI281363050, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652224, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652222, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652220, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652218, Length=463, Percent_Identity=36.2850971922246, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 53915; Mature: 53784

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVKIVDLTLRDGHQSLLATRMRTRDMLPILETLDESGIYSLEVWGGATFDACHRFLNE
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCC
NPWERLREIRKRVKNAKLQMLLRGQNLVGYRHYPDDIVEKFVQKTIENGLDVFRIFDALN
CHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
DVRNLISSIKAAKKYNADHIQGTICYTISPVHTVEKYVEIANELAALEVDSICLKDMAGM
HHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPKMAYELVKALKKEVGLPINVHSHYTSGMASMALLKGVEAGAEMIDTCMSPLSSGTSH
HCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPTESMVYALNELGYDTGVKLDILLEAREYFMKIREKYSGYLNPLSTIPDTRVLVYQIPG
CCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCC
GMFSNLIAQLQEQNALDRLQEVLEEVPRVREDLGYPPLVTPTSQIVGVQAVINVLVGERY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCH
KVVTRETKDLVKGMYGRTPAPIKPEIVKKILGDEKPIDCRPADLLEPEFEKRKQELIEAG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHC
IENPSDEDVLLYALFPQTGLKFLKGEMVEEPFPVAGGKIEGSFEVEIDGQKYVVKVAPEK
CCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEECCEEEEEECCCEEEEEECCCC
>Mature Secondary Structure 
AKVKIVDLTLRDGHQSLLATRMRTRDMLPILETLDESGIYSLEVWGGATFDACHRFLNE
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCC
NPWERLREIRKRVKNAKLQMLLRGQNLVGYRHYPDDIVEKFVQKTIENGLDVFRIFDALN
CHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
DVRNLISSIKAAKKYNADHIQGTICYTISPVHTVEKYVEIANELAALEVDSICLKDMAGM
HHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPKMAYELVKALKKEVGLPINVHSHYTSGMASMALLKGVEAGAEMIDTCMSPLSSGTSH
HCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PPTESMVYALNELGYDTGVKLDILLEAREYFMKIREKYSGYLNPLSTIPDTRVLVYQIPG
CCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCC
GMFSNLIAQLQEQNALDRLQEVLEEVPRVREDLGYPPLVTPTSQIVGVQAVINVLVGERY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCH
KVVTRETKDLVKGMYGRTPAPIKPEIVKKILGDEKPIDCRPADLLEPEFEKRKQELIEAG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHC
IENPSDEDVLLYALFPQTGLKFLKGEMVEEPFPVAGGKIEGSFEVEIDGQKYVVKVAPEK
CCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEECCEEEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]