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The map label for this gene is pgk

Identifier: 11498746

GI number: 11498746

Start: 1021803

End: 1023026

Strand: Reverse

Name: pgk

Synonym: AF1146

Alternate gene names: 11498746

Gene position: 1023026-1021803 (Counterclockwise)

Preceding gene: 11498748

Following gene: 11498745

Centisome position: 46.96

GC content: 49.84

Gene sequence:

>1224_bases
GTGATGATAGACGGCCTTCCAACACTAGACGATATTCCGTACAGAGGAAAGCACGTTCTGCTGAGGGTTGACATCAACGC
TCCTATTGTGAACTCAACGATTCTCGACACGAGCAGATTCGAGAGCCACATTCCGACAATTGAGGCTCTTGAAGACTCAA
AGCTCGTTTTGCTCGCACACCAGAGCCGACCCGGAAAGAAGGACTTCACGAGTCTCGAAAGCCACGCATCCACACTTTCC
AAACTGCTCGGGAAGAGAGTCGAGTACATAGACGAAATTTTCAGCAAAGGCGTTCTGAGAAGAATAAAGGAGATGGAGAA
CGGTGAGGTAATTTTGCTCGAGAACGTGAGGTTCTACTCCGAGGAGCAGTTGAACAGAAGCGCTGAGGAGCATGCCGAGT
GCCACATGGTCAGGAAACTCAGCACAGCCTTTGACCTCTTTGTCAACGACGCCTTCTCCGCATCCCATCGAAGCCACGCA
TCTTTGGTCGGCTTCGTTCCGGTTCTGCCATCCGTCGTGGGAAGGCTCGTCGAAAATGAGGTTACCGCCCTCAGCAAACC
CCTCAAGGGGGAGGGGAGAAAGATATTTGTCCTGGGCGGAGCTAAAATCAAGGACTCGGTGAAAGTTCTAAAAAACGTGC
TGGAAAACAACATTGCGGAAAAAGTCGTTCTCACGGGTGTAGTTGCCAACTACTTTCTCATGCTGAAAGGTTACGATATA
GGTGAAGTGAACAGAAAGGTTGTGGAGGACAACAAGGAAGACGTGAGCGACGAGGAGATGATAAACATACTCAAGAAGTA
CTCCGACAAAATTATCCTTCCCATTGACCTCGGTATAGAGAAAGACGGTGTCAGGGTAGATATCCCATTGGAGAAGTTTG
ATGGGAAATACAGAATAATGGATATTGGGCTTGAAACCGTTAACCAGCTTTCGGAAATCATCCCGAAGTACGATTATGTG
GTTCTGAACGGTCCTGCGGGAGTTTTTGAGGATGAGCGTTTCTCTCTCGGAACTTACGAGATTCTGAGGGCCGCGACAAG
GGCTGGTTACTCGGTTGTTGGCGGGGGGCACATCGCCTCAGCAGCACGCCTCTTCGGCCTTTCGGACAAGTTCAGCCACA
TATCAACTGCAGGAGGGGCGTGCATAAGGTTCCTGAGCGGTGAAAAGCTGGTAGCTCTTGAGGTTATCAAGGAATACTGG
GCGAAGAAGTGGGGCAAATCTTAA

Upstream 100 bases:

>100_bases
TTGCAGAGGACACCGCCAATGTCCGTTATTTCCATGAACCAATTCTGAGCAGGTAAATTAAAATTTTCAGAAAGTTTTTA
ATTTCACCTTCCGAATTGAA

Downstream 100 bases:

>100_bases
TTTTTTAAAATTTCATACGATAAAATTTTTAAGCTGCCCATTTTCCTAAAGGATTAATCAGAGTTATGTTTAATTCAGAA
AACGTTAAATATTCTCCATA

Product: 3-phosphoglycerate kinase (pgk)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MMIDGLPTLDDIPYRGKHVLLRVDINAPIVNSTILDTSRFESHIPTIEALEDSKLVLLAHQSRPGKKDFTSLESHASTLS
KLLGKRVEYIDEIFSKGVLRRIKEMENGEVILLENVRFYSEEQLNRSAEEHAECHMVRKLSTAFDLFVNDAFSASHRSHA
SLVGFVPVLPSVVGRLVENEVTALSKPLKGEGRKIFVLGGAKIKDSVKVLKNVLENNIAEKVVLTGVVANYFLMLKGYDI
GEVNRKVVEDNKEDVSDEEMINILKKYSDKIILPIDLGIEKDGVRVDIPLEKFDGKYRIMDIGLETVNQLSEIIPKYDYV
VLNGPAGVFEDERFSLGTYEILRAATRAGYSVVGGGHIASAARLFGLSDKFSHISTAGGACIRFLSGEKLVALEVIKEYW
AKKWGKS

Sequences:

>Translated_407_residues
MMIDGLPTLDDIPYRGKHVLLRVDINAPIVNSTILDTSRFESHIPTIEALEDSKLVLLAHQSRPGKKDFTSLESHASTLS
KLLGKRVEYIDEIFSKGVLRRIKEMENGEVILLENVRFYSEEQLNRSAEEHAECHMVRKLSTAFDLFVNDAFSASHRSHA
SLVGFVPVLPSVVGRLVENEVTALSKPLKGEGRKIFVLGGAKIKDSVKVLKNVLENNIAEKVVLTGVVANYFLMLKGYDI
GEVNRKVVEDNKEDVSDEEMINILKKYSDKIILPIDLGIEKDGVRVDIPLEKFDGKYRIMDIGLETVNQLSEIIPKYDYV
VLNGPAGVFEDERFSLGTYEILRAATRAGYSVVGGGHIASAARLFGLSDKFSHISTAGGACIRFLSGEKLVALEVIKEYW
AKKWGKS
>Mature_407_residues
MMIDGLPTLDDIPYRGKHVLLRVDINAPIVNSTILDTSRFESHIPTIEALEDSKLVLLAHQSRPGKKDFTSLESHASTLS
KLLGKRVEYIDEIFSKGVLRRIKEMENGEVILLENVRFYSEEQLNRSAEEHAECHMVRKLSTAFDLFVNDAFSASHRSHA
SLVGFVPVLPSVVGRLVENEVTALSKPLKGEGRKIFVLGGAKIKDSVKVLKNVLENNIAEKVVLTGVVANYFLMLKGYDI
GEVNRKVVEDNKEDVSDEEMINILKKYSDKIILPIDLGIEKDGVRVDIPLEKFDGKYRIMDIGLETVNQLSEIIPKYDYV
VLNGPAGVFEDERFSLGTYEILRAATRAGYSVVGGGHIASAARLFGLSDKFSHISTAGGACIRFLSGEKLVALEVIKEYW
AKKWGKS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=428, Percent_Identity=30.8411214953271, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI31543397, Length=428, Percent_Identity=31.3084112149533, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1789294, Length=414, Percent_Identity=30.1932367149758, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=31.9612590799031, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319857, Length=424, Percent_Identity=29.9528301886792, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=32.0481927710843, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=30.5288461538462, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ARCFU (O29119)

Other databases:

- EMBL:   AE000782
- PIR:   A69393
- RefSeq:   NP_069975.1
- ProteinModelPortal:   O29119
- SMR:   O29119
- GeneID:   1484370
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1146
- NMPDR:   fig|224325.1.peg.1134
- TIGR:   AF_1146
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- ProtClustDB:   CLSK2746771
- BioCyc:   AFUL224325:AF_1146-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 45279; Mature: 45279

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 39-39 BINDING 117-117 BINDING 157-157 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIDGLPTLDDIPYRGKHVLLRVDINAPIVNSTILDTSRFESHIPTIEALEDSKLVLLAH
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECCHHCCHHHHHHHCCCHHCCCCCCEEEEEE
QSRPGKKDFTSLESHASTLSKLLGKRVEYIDEIFSKGVLRRIKEMENGEVILLENVRFYS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
EEQLNRSAEEHAECHMVRKLSTAFDLFVNDAFSASHRSHASLVGFVPVLPSVVGRLVENE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTALSKPLKGEGRKIFVLGGAKIKDSVKVLKNVLENNIAEKVVLTGVVANYFLMLKGYDI
HHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
GEVNRKVVEDNKEDVSDEEMINILKKYSDKIILPIDLGIEKDGVRVDIPLEKFDGKYRIM
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECHHHCCCCEEEE
DIGLETVNQLSEIIPKYDYVVLNGPAGVFEDERFSLGTYEILRAATRAGYSVVGGGHIAS
EECHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCHHHH
AARLFGLSDKFSHISTAGGACIRFLSGEKLVALEVIKEYWAKKWGKS
HHHHHCCCHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMIDGLPTLDDIPYRGKHVLLRVDINAPIVNSTILDTSRFESHIPTIEALEDSKLVLLAH
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECCHHCCHHHHHHHCCCHHCCCCCCEEEEEE
QSRPGKKDFTSLESHASTLSKLLGKRVEYIDEIFSKGVLRRIKEMENGEVILLENVRFYS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
EEQLNRSAEEHAECHMVRKLSTAFDLFVNDAFSASHRSHASLVGFVPVLPSVVGRLVENE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTALSKPLKGEGRKIFVLGGAKIKDSVKVLKNVLENNIAEKVVLTGVVANYFLMLKGYDI
HHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
GEVNRKVVEDNKEDVSDEEMINILKKYSDKIILPIDLGIEKDGVRVDIPLEKFDGKYRIM
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECHHHCCCCEEEE
DIGLETVNQLSEIIPKYDYVVLNGPAGVFEDERFSLGTYEILRAATRAGYSVVGGGHIAS
EECHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCHHHH
AARLFGLSDKFSHISTAGGACIRFLSGEKLVALEVIKEYWAKKWGKS
HHHHHCCCHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475