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Identifier: 11498485

GI number: 11498485

Start: 781323

End: 782447

Strand: Reverse

Name: Not Available

Synonym: AF0879

Alternate gene names: 11498485

Gene position: 782447-781323 (Counterclockwise)

Preceding gene: 11498487

Following gene: 11498484

Centisome position: 35.92

GC content: 47.02

Gene sequence:

>1125_bases
ATGATGGAAAAGGTGGCAGTGGTTCACTACGGTGAGATTGGTATCAAGGGCAAAAACAGGGAGTTTTTCGAGAGGAAGTT
GATTGAGGCAATAAAAAGGGCCACCGGCATGAGGGTTAGAAGAAAATACGGGAGAATAGAGGTGGAGTACAGAGAAGGTC
TGGAAAGCAGGATGGCGAAAATTCCAGGAATAAGGTATTTTGGTGTTGGGTTTAAAACCGAACTCAACCTTGAATCAATT
AAAAAGGCAGCTTTAAGTGTGCTGCCCGAAAGCTTCAAAACCTTCAAGGTTGATACTTCGAGAAGCAACAAAAACTTCCC
AATGAACTCCGTTGAAGTCAACAGAAAAGTTGGAGCGTTCATTGTGGAAAAGACGGGAAAGAAGGTCGATTTAACGAATC
CTGACGTAACAGTTTGGATTGAAATCTGCGAAAAGGAGGCTTACGTTTACTCGAAGAGGTACGAGGGGATTGGTGGCCTG
CCGGTTGGTGTTGCCGGAAAAGTTGTTGCTCTCATTTCAGGAGGGATAGACAGTCCCGTTGCTGCATTTATGGCGATGAA
GAGGGGCTGTGAGGTGGTGGCTGTCCACTTCTTCAACCAGACCATGCACTCCCCAAAGGTGAGGGAGAAGATAAGGATGC
TTGCTGAGAAGCTTGCTGAGTACCAAGGAAAAATTAAGCTTTACATGGTGCCGTTTAAGGAGGTGCAGTTTGAGATAATC
AACAGGGTTCCGCCCAAGCTGAGGATGGTTGTTTACAGAAGGAGCATGATGAGAATGGCCAACCTTATTGCAGAAAAGGA
GGGATGTAAGGCCGTAGTCACGGGAGACAGCCTCTCTCAGGTTGCGAGCCAGACGCTCGACAACATCAATGTCATTTACT
CAGCCTCAAAGCTTGCGGTTCTTCCACCACTGATAGGAATGGACAAGGAGGAGATTGTTGCCATTGCAAAGAGAATTGAA
ACTTACGAGATTTCCATTCTTCCCTACGAGGACTGCTGCAGCTTCATGGTTGCCCAGCATCCCGAGACAAGGGCGAAAAT
TGAAGACGTCGTTAAATACGAGGGTTTTGAGGAACTTGAAAGGAAAGCGGTAGAGGAGAGCGAGATTCTGGAGGTGGGAA
GCTGA

Upstream 100 bases:

>100_bases
TAAACGTATCCGCAAATCGTGCACTGATACTTTGCCATACCCAATCATGTTTCTTCTCAGTTATAATCCTTTCGCTTAAA
TATCGCCTCAACAACATTGA

Downstream 100 bases:

>100_bases
ATCTTGAAGAGTGGATGGAGATTTACGAGCAAATTCTCAGGGATTTCGGATTCAGCAGGGAAGAGGATGAGAGGGCTGCA
AGGCTGATGAAGGAGCTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MMEKVAVVHYGEIGIKGKNREFFERKLIEAIKRATGMRVRRKYGRIEVEYREGLESRMAKIPGIRYFGVGFKTELNLESI
KKAALSVLPESFKTFKVDTSRSNKNFPMNSVEVNRKVGAFIVEKTGKKVDLTNPDVTVWIEICEKEAYVYSKRYEGIGGL
PVGVAGKVVALISGGIDSPVAAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIKLYMVPFKEVQFEII
NRVPPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQVASQTLDNINVIYSASKLAVLPPLIGMDKEEIVAIAKRIE
TYEISILPYEDCCSFMVAQHPETRAKIEDVVKYEGFEELERKAVEESEILEVGS

Sequences:

>Translated_374_residues
MMEKVAVVHYGEIGIKGKNREFFERKLIEAIKRATGMRVRRKYGRIEVEYREGLESRMAKIPGIRYFGVGFKTELNLESI
KKAALSVLPESFKTFKVDTSRSNKNFPMNSVEVNRKVGAFIVEKTGKKVDLTNPDVTVWIEICEKEAYVYSKRYEGIGGL
PVGVAGKVVALISGGIDSPVAAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIKLYMVPFKEVQFEII
NRVPPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQVASQTLDNINVIYSASKLAVLPPLIGMDKEEIVAIAKRIE
TYEISILPYEDCCSFMVAQHPETRAKIEDVVKYEGFEELERKAVEESEILEVGS
>Mature_374_residues
MMEKVAVVHYGEIGIKGKNREFFERKLIEAIKRATGMRVRRKYGRIEVEYREGLESRMAKIPGIRYFGVGFKTELNLESI
KKAALSVLPESFKTFKVDTSRSNKNFPMNSVEVNRKVGAFIVEKTGKKVDLTNPDVTVWIEICEKEAYVYSKRYEGIGGL
PVGVAGKVVALISGGIDSPVAAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIKLYMVPFKEVQFEII
NRVPPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQVASQTLDNINVIYSASKLAVLPPLIGMDKEEIVAIAKRIE
TYEISILPYEDCCSFMVAQHPETRAKIEDVVKYEGFEELERKAVEESEILEVGS

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=353, Percent_Identity=32.8611898016997, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_ARCFU (O29382)

Other databases:

- EMBL:   AE000782
- PIR:   G69359
- RefSeq:   NP_069713.1
- ProteinModelPortal:   O29382
- SMR:   O29382
- GeneID:   1484101
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0879
- NMPDR:   fig|224325.1.peg.872
- TIGR:   AF_0879
- HOGENOM:   HBG646072
- OMA:   REISVEM
- ProtClustDB:   CLSK2746641
- BioCyc:   AFUL224325:AF_0879-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 42273; Mature: 42273

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS51165 THUMP

Important sites: BINDING 253-253 BINDING 275-275 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEKVAVVHYGEIGIKGKNREFFERKLIEAIKRATGMRVRRKYGRIEVEYREGLESRMAK
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHCHHHHHHH
IPGIRYFGVGFKTELNLESIKKAALSVLPESFKTFKVDTSRSNKNFPMNSVEVNRKVGAF
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEECCCHHCEE
IVEKTGKKVDLTNPDVTVWIEICEKEAYVYSKRYEGIGGLPVGVAGKVVALISGGIDSPV
EEECCCCEEECCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHH
AAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIKLYMVPFKEVQFEII
HHHHHHHCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
NRVPPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQVASQTLDNINVIYSASKLAV
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCEEEEECCCCEE
LPPLIGMDKEEIVAIAKRIETYEISILPYEDCCSFMVAQHPETRAKIEDVVKYEGFEELE
CCCCCCCCHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
RKAVEESEILEVGS
HHHCCHHHHHCCCC
>Mature Secondary Structure
MMEKVAVVHYGEIGIKGKNREFFERKLIEAIKRATGMRVRRKYGRIEVEYREGLESRMAK
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHCHHHHHHH
IPGIRYFGVGFKTELNLESIKKAALSVLPESFKTFKVDTSRSNKNFPMNSVEVNRKVGAF
CCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEECCCHHCEE
IVEKTGKKVDLTNPDVTVWIEICEKEAYVYSKRYEGIGGLPVGVAGKVVALISGGIDSPV
EEECCCCEEECCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHH
AAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKIKLYMVPFKEVQFEII
HHHHHHHCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
NRVPPKLRMVVYRRSMMRMANLIAEKEGCKAVVTGDSLSQVASQTLDNINVIYSASKLAV
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCEEEEECCCCEE
LPPLIGMDKEEIVAIAKRIETYEISILPYEDCCSFMVAQHPETRAKIEDVVKYEGFEELE
CCCCCCCCHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
RKAVEESEILEVGS
HHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475