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The map label for this gene is asnA

Identifier: 11498487

GI number: 11498487

Start: 785540

End: 786796

Strand: Reverse

Name: asnA

Synonym: AF0882

Alternate gene names: 11498487

Gene position: 786796-785540 (Counterclockwise)

Preceding gene: 11498488

Following gene: 11498485

Centisome position: 36.12

GC content: 50.44

Gene sequence:

>1257_bases
ATGAGGCCAAAAAGCTGGGGGTGGATATCAATGTCTGAAAGGCTTGAAGGCAAGAGAGTCAGAATAAAGGCAAAGGGAAA
GATTTTTGAAGGCATAGTGATGCCCTCCTTTACCGGAAACTTTGTCCTGAAGCTTGATAATGGCTACAATGTTGGATTTA
AGGAGTACGAGCTTCTCGAAGTCCTTGAGGTTGAACCCTTCGAACCCCATCTTCCTGAACTGGTTAAGAGGGAGGGATTG
CCGGATGTCAAGATTATCTCAACGGGAGGCACTATAGCCAGCAAGGTTGATTACAGAACCGGAGCGGTAACAAGCCAGTT
CACAGCGGAGGAAATAGCCTCGGAAGTGCCTGAGCTAACCGAAATCTGCAATGTTGATGCCGAGCTGCTTTACAACATTC
TGAGCGAGAATATGAAGCCAGAGAATTGGATTGAGCTTGCAAGGCACGTCTACAAAGCTTTAAAAGACCATGAGGGAGTA
ATAATCACCCACGGCACCGATACAATGCACTTCTCCGCTGCTGCGCTTTCATTTATGCTCTCAACACCAAAGCCCGTGGT
TTTTGTTGGTGCCCAGAGAAGCTCCGACAGGCCCAGCAGCGATGCTGCGATGAACTTGCTGTGTGCTGCGAAGGCAGCAA
CAGAGGACATTGGCGAGGTGGTGGTTTGCATGCACGGCTCGACAAGTGATGATTACTGCTTAGTCCACCGCGGTGTGAAA
GTAAGGAAGAACCACACCTCCAGAAGGGATGCCTTTCAGTCTGTAAACGCAAAGCCCATAGGCAGAATCGACTACCCCTC
GCTCAGCGTTGAGTGGCTTTCTTGGCGTTACCGCAGGGGTGAGAGGGAGTTGAAGCTTACGGACAGGCTTGAGAGGAAAG
TAGTCCTCATCAAGTTCTTCCCCGGGCTGAGCAGCGACATCCTCGAATACTACCACTCCAAGGGTTACAGGGGATTTGTT
ATCGAGGGGACTGGATTGGGCCACGTTTCTACTGATTGGATTGACACGCTAAGAAGAGTTTGCGAGGACTCCGTTGTTGT
GATGACCTCCCAGTGCCTCTGGGGGAGGGTCTGCGATAGAGTTTACGACACCGGAAGGGACATTCTGAGGGCAGGAGTAA
TAGAGGGAGAGGACATGCTTCCAGAAGTCGCCCTAATAAAGCTCATGTGGCTGCTTGGAAACTACAGCATAGAAGAGGCG
AAAGAAATGGTGAAGAAGAGTGTCGCCGGCGAAATAGAGCCGACGACACAGTATTAG

Upstream 100 bases:

>100_bases
GAGAGAATGATAAAAGCGAGGAAAAGCGATCTGAAAGACAGGAAAACTTTGTTAAGGAAGCTTAGGGGAGAGGTTAAAAT
GGGGCTTAAAATGCTTTACG

Downstream 100 bases:

>100_bases
CCTCTGCTCTTCTTCCCCCTCTTGGCTATCACCTCCAATCTAATTTAACCAAAAAATCATTAATAAATTTTTTGCTCAAA
ATTGCAATTACTTTCAGATT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRPKSWGWISMSERLEGKRVRIKAKGKIFEGIVMPSFTGNFVLKLDNGYNVGFKEYELLEVLEVEPFEPHLPELVKREGL
PDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELTEICNVDAELLYNILSENMKPENWIELARHVYKALKDHEGV
IITHGTDTMHFSAAALSFMLSTPKPVVFVGAQRSSDRPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVK
VRKNHTSRRDAFQSVNAKPIGRIDYPSLSVEWLSWRYRRGERELKLTDRLERKVVLIKFFPGLSSDILEYYHSKGYRGFV
IEGTGLGHVSTDWIDTLRRVCEDSVVVMTSQCLWGRVCDRVYDTGRDILRAGVIEGEDMLPEVALIKLMWLLGNYSIEEA
KEMVKKSVAGEIEPTTQY

Sequences:

>Translated_418_residues
MRPKSWGWISMSERLEGKRVRIKAKGKIFEGIVMPSFTGNFVLKLDNGYNVGFKEYELLEVLEVEPFEPHLPELVKREGL
PDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELTEICNVDAELLYNILSENMKPENWIELARHVYKALKDHEGV
IITHGTDTMHFSAAALSFMLSTPKPVVFVGAQRSSDRPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVK
VRKNHTSRRDAFQSVNAKPIGRIDYPSLSVEWLSWRYRRGERELKLTDRLERKVVLIKFFPGLSSDILEYYHSKGYRGFV
IEGTGLGHVSTDWIDTLRRVCEDSVVVMTSQCLWGRVCDRVYDTGRDILRAGVIEGEDMLPEVALIKLMWLLGNYSIEEA
KEMVKKSVAGEIEPTTQY
>Mature_418_residues
MRPKSWGWISMSERLEGKRVRIKAKGKIFEGIVMPSFTGNFVLKLDNGYNVGFKEYELLEVLEVEPFEPHLPELVKREGL
PDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELTEICNVDAELLYNILSENMKPENWIELARHVYKALKDHEGV
IITHGTDTMHFSAAALSFMLSTPKPVVFVGAQRSSDRPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVK
VRKNHTSRRDAFQSVNAKPIGRIDYPSLSVEWLSWRYRRGERELKLTDRLERKVVLIKFFPGLSSDILEYYHSKGYRGFV
IEGTGLGHVSTDWIDTLRRVCEDSVVVMTSQCLWGRVCDRVYDTGRDILRAGVIEGEDMLPEVALIKLMWLLGNYSIEEA
KEMVKKSVAGEIEPTTQY

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=309, Percent_Identity=29.7734627831715, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788065, Length=293, Percent_Identity=30.0341296928328, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789327, Length=306, Percent_Identity=29.4117647058824, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193207372, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558280, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193207374, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558278, Length=286, Percent_Identity=30.0699300699301, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323189, Length=267, Percent_Identity=28.8389513108614, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323187, Length=267, Percent_Identity=28.8389513108614, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323186, Length=267, Percent_Identity=28.8389513108614, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323184, Length=267, Percent_Identity=28.8389513108614, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320527, Length=337, Percent_Identity=24.6290801186944, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639609, Length=316, Percent_Identity=31.0126582278481, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24645530, Length=281, Percent_Identity=32.0284697508897, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_ARCFU (O29380)

Other databases:

- EMBL:   AE000782
- PIR:   B69360
- RefSeq:   NP_069715.1
- ProteinModelPortal:   O29380
- SMR:   O29380
- GeneID:   1484104
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0882
- NMPDR:   fig|224325.1.peg.874
- TIGR:   AF_0882
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- ProtClustDB:   PRK04183
- BioCyc:   AFUL224325:AF_0882-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 47092; Mature: 47092

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 91-91 ACT_SITE 166-166 ACT_SITE 167-167 ACT_SITE 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKSWGWISMSERLEGKRVRIKAKGKIFEGIVMPSFTGNFVLKLDNGYNVGFKEYELLE
CCCCCCCEEEHHHCCCCCEEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHH
VLEVEPFEPHLPELVKREGLPDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELT
HHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHCHHHH
EICNVDAELLYNILSENMKPENWIELARHVYKALKDHEGVIITHGTDTMHFSAAALSFML
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
STPKPVVFVGAQRSSDRPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVK
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCEE
VRKNHTSRRDAFQSVNAKPIGRIDYPSLSVEWLSWRYRRGERELKLTDRLERKVVLIKFF
EECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEEEC
PGLSSDILEYYHSKGYRGFVIEGTGLGHVSTDWIDTLRRVCEDSVVVMTSQCLWGRVCDR
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VYDTGRDILRAGVIEGEDMLPEVALIKLMWLLGNYSIEEAKEMVKKSVAGEIEPTTQY
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRPKSWGWISMSERLEGKRVRIKAKGKIFEGIVMPSFTGNFVLKLDNGYNVGFKEYELLE
CCCCCCCEEEHHHCCCCCEEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHH
VLEVEPFEPHLPELVKREGLPDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELT
HHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHCHHHH
EICNVDAELLYNILSENMKPENWIELARHVYKALKDHEGVIITHGTDTMHFSAAALSFML
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
STPKPVVFVGAQRSSDRPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVK
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCEE
VRKNHTSRRDAFQSVNAKPIGRIDYPSLSVEWLSWRYRRGERELKLTDRLERKVVLIKFF
EECCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEEEC
PGLSSDILEYYHSKGYRGFVIEGTGLGHVSTDWIDTLRRVCEDSVVVMTSQCLWGRVCDR
CCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VYDTGRDILRAGVIEGEDMLPEVALIKLMWLLGNYSIEEAKEMVKKSVAGEIEPTTQY
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475