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The map label for this gene is glpK

Identifier: 11498472

GI number: 11498472

Start: 770576

End: 772054

Strand: Reverse

Name: glpK

Synonym: AF0866

Alternate gene names: 11498472

Gene position: 772054-770576 (Counterclockwise)

Preceding gene: 11498473

Following gene: 11498471

Centisome position: 35.44

GC content: 51.66

Gene sequence:

>1479_bases
ATGATTGGGGTAATCGATGCGGGAACAACAACGATAAAGCTTGCCGTTTACGATGAGGATAAACTTGTAGCGATCAAAAA
GGAGCCGGTTGTCAAGCACAATCCAAAGCCGGGATGGGTTGAGATCGATGCAGAGGATTTAGCGAGGAAGTGTGTGAGCT
TCGCTGACACAGCCATTGACGAGTATGGTGTTGAGGTAATTGCCATAACCAACCAGCGAACCACCGCAGTTCTGTGGGAT
GGTAAAACGGGCAGGCCAGTCTTCAATGCCCTTGGATGGCAGGACATGAGGGCCAACGCTCTTGCTGAGGAGATGAACAG
AGACTCTACCATCAGGATGGCAAGGACTGCTGGAATGATTGCGAGAGGGGTGGTGAAGCTGCTTCCAACGCTGAAAAACA
AGAGAAGGGTTAAGTGGCTCATCACACTCTCCCGCCTCTCAATCCGCCCCAACCACACCAGCGTGAAGCTCTGCTGGATG
CTGAGGGAGCTTGGAGAGAAAAAGGAGAAGTATGATCTGAAAGCTGGAACCGTTGACAGCTGGCTTGTATACAGGCTGAC
AGGTGAGCATCTTACAGATTACTCCAATGCTGCTGCTACAGGCCTCTACGACTCCTACTACCTGCGGTGGAGCGAGCCCA
TTCTAAAAATTGTTGGAGCAGATGAGGAAATGCTTCCGAAAACCCTCGAAAGCGACAGAATCTTTGGAGAGTACAGAAAT
GTGCCAGTCACTGGAGTCATCGCCGACCAATCCGCCTCCCTCTACGCCCTCGGATGCTGGGAGGAGGGAGACATCAAGGC
CACCAACGGAACCGGAACCTTCGTCGACCTCAATGTTGGAGAAGAGCCCCAAGCCTCTCCCGGAGGTTTGCTTCCCCTCA
TAGCGTGGAAGCTGAAGTCGGAAATGAGATACATGATGGAGGGGATGCTCTTCTACAGCGGTTCTGCCGTGGAGAAGCTG
AAGGAAATCGGCATCTACGATGATGTTTCTAAAACTTCAGAAATGGCCTTCAGGTCGAAGAATGACGACATGCTGCTCAT
CCCCTCCTTCACCGGCCTCGCAACTCCCCACTACGTCTCGGTTCCCGGCTTGCTTTACGGGATATCCAACGCCATGACGA
GGGAGGATATAGTGAAAGCCCTGCTCGAATCCATAGCCTTCAGAATAGCCGAAATCGTCGAGATTATGCGGAAGGAGTTT
CCCTACGAAACTGACAGAATCAGATGCGACGGAGAGATGTCCTCCAACGACTTCTTCCTGCAGAGAATTGCCGACGTTAC
AGGCTTGAAAGTTGAGAGGGGGGCAGTGCTTTCGGGAACTTCTTTCGGGGCGCACCTCGTTGCTGGGAGGGCTTTGGGCA
AGTGGAAAAAGGACTTCTGCATGCCCTTCGATAAAGTTTTCGAACCCTCTTTGGATTTATCGGAGAAGTACAGAAGGTGG
AAGAGGCTGCTTGAAATATCGAAAAAGCTTAAGGTGTGA

Upstream 100 bases:

>100_bases
GGAGGATGGAGCACTTTGAGCATCAAATCGTGACTCTCTGCCCCTCGTGCAGGATGGCGCTGAAGGCAAAGGATATTGTT
GAGATTATAGCTGAGGGCCT

Downstream 100 bases:

>100_bases
AAAATTTTAGCTGTGGGGAAAAATTTTTAAAGATAATTATCAACTTGCATCATGCCGTATGCTGACAACGGTGTTAAAAT
TTACTACGAGGTTGAGGATG

Product: glycerol kinase (glpK)

Products: ADP; sn-glycerol 3-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIGVIDAGTTTIKLAVYDEDKLVAIKKEPVVKHNPKPGWVEIDAEDLARKCVSFADTAIDEYGVEVIAITNQRTTAVLWD
GKTGRPVFNALGWQDMRANALAEEMNRDSTIRMARTAGMIARGVVKLLPTLKNKRRVKWLITLSRLSIRPNHTSVKLCWM
LRELGEKKEKYDLKAGTVDSWLVYRLTGEHLTDYSNAAATGLYDSYYLRWSEPILKIVGADEEMLPKTLESDRIFGEYRN
VPVTGVIADQSASLYALGCWEEGDIKATNGTGTFVDLNVGEEPQASPGGLLPLIAWKLKSEMRYMMEGMLFYSGSAVEKL
KEIGIYDDVSKTSEMAFRSKNDDMLLIPSFTGLATPHYVSVPGLLYGISNAMTREDIVKALLESIAFRIAEIVEIMRKEF
PYETDRIRCDGEMSSNDFFLQRIADVTGLKVERGAVLSGTSFGAHLVAGRALGKWKKDFCMPFDKVFEPSLDLSEKYRRW
KRLLEISKKLKV

Sequences:

>Translated_492_residues
MIGVIDAGTTTIKLAVYDEDKLVAIKKEPVVKHNPKPGWVEIDAEDLARKCVSFADTAIDEYGVEVIAITNQRTTAVLWD
GKTGRPVFNALGWQDMRANALAEEMNRDSTIRMARTAGMIARGVVKLLPTLKNKRRVKWLITLSRLSIRPNHTSVKLCWM
LRELGEKKEKYDLKAGTVDSWLVYRLTGEHLTDYSNAAATGLYDSYYLRWSEPILKIVGADEEMLPKTLESDRIFGEYRN
VPVTGVIADQSASLYALGCWEEGDIKATNGTGTFVDLNVGEEPQASPGGLLPLIAWKLKSEMRYMMEGMLFYSGSAVEKL
KEIGIYDDVSKTSEMAFRSKNDDMLLIPSFTGLATPHYVSVPGLLYGISNAMTREDIVKALLESIAFRIAEIVEIMRKEF
PYETDRIRCDGEMSSNDFFLQRIADVTGLKVERGAVLSGTSFGAHLVAGRALGKWKKDFCMPFDKVFEPSLDLSEKYRRW
KRLLEISKKLKV
>Mature_492_residues
MIGVIDAGTTTIKLAVYDEDKLVAIKKEPVVKHNPKPGWVEIDAEDLARKCVSFADTAIDEYGVEVIAITNQRTTAVLWD
GKTGRPVFNALGWQDMRANALAEEMNRDSTIRMARTAGMIARGVVKLLPTLKNKRRVKWLITLSRLSIRPNHTSVKLCWM
LRELGEKKEKYDLKAGTVDSWLVYRLTGEHLTDYSNAAATGLYDSYYLRWSEPILKIVGADEEMLPKTLESDRIFGEYRN
VPVTGVIADQSASLYALGCWEEGDIKATNGTGTFVDLNVGEEPQASPGGLLPLIAWKLKSEMRYMMEGMLFYSGSAVEKL
KEIGIYDDVSKTSEMAFRSKNDDMLLIPSFTGLATPHYVSVPGLLYGISNAMTREDIVKALLESIAFRIAEIVEIMRKEF
PYETDRIRCDGEMSSNDFFLQRIADVTGLKVERGAVLSGTSFGAHLVAGRALGKWKKDFCMPFDKVFEPSLDLSEKYRRW
KRLLEISKKLKV

Specific function: Key Enzyme In The Regulation Of Glycerol Uptake And Metabolism. [C]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI88196792, Length=473, Percent_Identity=30.8668076109937, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI41393575, Length=459, Percent_Identity=28.322440087146, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI189409120, Length=459, Percent_Identity=28.1045751633987, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4504007, Length=459, Percent_Identity=27.8867102396514, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI42794763, Length=465, Percent_Identity=27.5268817204301, Blast_Score=192, Evalue=6e-49,
Organism=Escherichia coli, GI1790361, Length=514, Percent_Identity=28.988326848249, Blast_Score=243, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17535599, Length=453, Percent_Identity=30.6843267108168, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI71986140, Length=522, Percent_Identity=28.1609195402299, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=286, Percent_Identity=32.5174825174825, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=510, Percent_Identity=23.7254901960784, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24655555, Length=523, Percent_Identity=30.2103250478011, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24655560, Length=523, Percent_Identity=30.2103250478011, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24655570, Length=523, Percent_Identity=30.2103250478011, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24655574, Length=523, Percent_Identity=30.2103250478011, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24655565, Length=523, Percent_Identity=30.2103250478011, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21357867, Length=538, Percent_Identity=28.996282527881, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656367, Length=531, Percent_Identity=29.9435028248588, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17864214, Length=523, Percent_Identity=28.8718929254302, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=29.7539149888143, Blast_Score=198, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24656372, Length=360, Percent_Identity=31.6666666666667, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI19922058, Length=549, Percent_Identity=24.9544626593807, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45552909, Length=305, Percent_Identity=30.1639344262295, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24652871, Length=481, Percent_Identity=25.987525987526, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): Y866_ARCFU (O29395)

Other databases:

- EMBL:   AE000782
- PIR:   B69358
- RefSeq:   NP_069700.1
- ProteinModelPortal:   O29395
- SMR:   O29395
- GeneID:   1484086
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0866
- NMPDR:   fig|224325.1.peg.859
- TIGR:   AF_0866
- HOGENOM:   HBG511469
- OMA:   YTNASAT
- ProtClustDB:   CLSK2502444
- BioCyc:   AFUL224325:AF_0866-MONOMER
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: 2.7.1.30

Molecular weight: Translated: 55260; Mature: 55260

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVIDAGTTTIKLAVYDEDKLVAIKKEPVVKHNPKPGWVEIDAEDLARKCVSFADTAID
CEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
EYGVEVIAITNQRTTAVLWDGKTGRPVFNALGWQDMRANALAEEMNRDSTIRMARTAGMI
HCCCEEEEEECCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
ARGVVKLLPTLKNKRRVKWLITLSRLSIRPNHTSVKLCWMLRELGEKKEKYDLKAGTVDS
HHHHHHHHHHHCCCCEEEEEEEEEHEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCC
WLVYRLTGEHLTDYSNAAATGLYDSYYLRWSEPILKIVGADEEMLPKTLESDRIFGEYRN
EEEEEEECCHHHCCCCHHHHCCCHHHEEEECCCHHEEECCCHHHHHHHHHCCCHHHHHCC
VPVTGVIADQSASLYALGCWEEGDIKATNGTGTFVDLNVGEEPQASPGGLLPLIAWKLKS
CCEEEEEECCCCCEEEEECCCCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
EMRYMMEGMLFYSGSAVEKLKEIGIYDDVSKTSEMAFRSKNDDMLLIPSFTGLATPHYVS
HHHHHHHHHHEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEE
VPGLLYGISNAMTREDIVKALLESIAFRIAEIVEIMRKEFPYETDRIRCDGEMSSNDFFL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
QRIADVTGLKVERGAVLSGTSFGAHLVAGRALGKWKKDFCMPFDKVFEPSLDLSEKYRRW
HHHHHHCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHH
KRLLEISKKLKV
HHHHHHHHHHCC
>Mature Secondary Structure
MIGVIDAGTTTIKLAVYDEDKLVAIKKEPVVKHNPKPGWVEIDAEDLARKCVSFADTAID
CEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
EYGVEVIAITNQRTTAVLWDGKTGRPVFNALGWQDMRANALAEEMNRDSTIRMARTAGMI
HCCCEEEEEECCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHH
ARGVVKLLPTLKNKRRVKWLITLSRLSIRPNHTSVKLCWMLRELGEKKEKYDLKAGTVDS
HHHHHHHHHHHCCCCEEEEEEEEEHEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCC
WLVYRLTGEHLTDYSNAAATGLYDSYYLRWSEPILKIVGADEEMLPKTLESDRIFGEYRN
EEEEEEECCHHHCCCCHHHHCCCHHHEEEECCCHHEEECCCHHHHHHHHHCCCHHHHHCC
VPVTGVIADQSASLYALGCWEEGDIKATNGTGTFVDLNVGEEPQASPGGLLPLIAWKLKS
CCEEEEEECCCCCEEEEECCCCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
EMRYMMEGMLFYSGSAVEKLKEIGIYDDVSKTSEMAFRSKNDDMLLIPSFTGLATPHYVS
HHHHHHHHHHEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEE
VPGLLYGISNAMTREDIVKALLESIAFRIAEIVEIMRKEFPYETDRIRCDGEMSSNDFFL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHH
QRIADVTGLKVERGAVLSGTSFGAHLVAGRALGKWKKDFCMPFDKVFEPSLDLSEKYRRW
HHHHHHCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHH
KRLLEISKKLKV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: KCl; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.9 {monothioglycerol}} 100 {dihydroxyacetone}} 0.5 {dihydroxyacetone}} 0.15 {glyceric} 42 {L-glyceraldehyde}} 3 {L-glyceraldehyde}} [C]

Substrates: ATP; glycerol

Specific reaction: ATP + glycerol = ADP + sn-glycerol 3-phosphate

General reaction: Phospho group transfer [C]

Inhibitor: ADP; DTNB; Fructose1, 6- diphosphate bacterialspecies, guanidine HCl; Iodoacetate; L- alpha-Glycerophosphate; N-Ethyl maleimide fructose1, 6-bisphosphate, propane-1, 3-diol; p-Hydroxy mercuriphenyl sulfonate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475