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The map label for this gene is est-1

Identifier: 11498471

GI number: 11498471

Start: 769724

End: 770524

Strand: Reverse

Name: est-1

Synonym: AF0865

Alternate gene names: 11498471

Gene position: 770524-769724 (Counterclockwise)

Preceding gene: 11498472

Following gene: 11498470

Centisome position: 35.37

GC content: 50.69

Gene sequence:

>801_bases
ATGCCGTATGCTGACAACGGTGTTAAAATTTACTACGAGGTTGAGGATGGAGGAGAGCCGGCAATCGTTTTCGTTCACGG
ATGGACCGCCAACATGAATTTCTGGAGGGAGCAGAGGGAGTACTTCAAAGGCAAACACAGAATGCTTTTCATCGACAACA
GAGGACACGGAAAGTCGGACAAGCCCTTCAACAGGAGCTTTTACGAGTTCGATAACTTCGTTTCCGACTTGCATGCCGCG
GTTAAGGATGCGAGTTTTGACAGATTCGTGCTCGTTGGGCACTCCTTCGGAACAATGATTTCGATGAGGTACTGCGTGGA
GCATCCTGGGAGGGTTGAAGCGCTTGTTTTGATAGGGGGTGGTGCGAGAATCCAGTCCCTGCACCGCTACGGCTACCCAA
TTGGAAGGCTTTTCGCTACATTGGCTTACGGAATCTCCGCAAGAATCATAGCAAACATGGCCTTCGGGAGAAAGGCTGGA
GAGCTGAGGGATTGGGGGCTGAAGGAGGCACTGGAAAACACTCCCAAACATGCAGCCCTTAACACTCTCTGGACTCTGAC
TACAGTGGATTTAAGGGACATTGCGAGAGAAATAGAGAAACCGACGCTGATAGTTGTCGGAAAGGAGGACGCACTGCTCC
CCGTAAGCAAGTCGGAGGAGCTTTCGAGGCTGATAAAGAATTCAAAGATGGTGATCGTTCCCGATGCCGGACACTGCGTG
ATGCTTGAGCAGCCTGAAATCGTTAACAGGGTTTTGGAGGAGTTCATACACACGTTTTCGGCGATGCTTATCAGAGCCTG
A

Upstream 100 bases:

>100_bases
CAGAAGGTGGAAGAGGCTGCTTGAAATATCGAAAAAGCTTAAGGTGTGAAAAATTTTAGCTGTGGGGAAAAATTTTTAAA
GATAATTATCAACTTGCATC

Downstream 100 bases:

>100_bases
GGTGGTTTGTTGGAGAGGCCAACCGGTGTAACTGTTTTGGCGATTCTGTATGTCTTGGCGGCTGTATTTTTCTTTCTAGC
AGCAGCGGTATCCGGATACC

Product: carboxylesterase (est-1)

Products: alcohol; carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPYADNGVKIYYEVEDGGEPAIVFVHGWTANMNFWREQREYFKGKHRMLFIDNRGHGKSDKPFNRSFYEFDNFVSDLHAA
VKDASFDRFVLVGHSFGTMISMRYCVEHPGRVEALVLIGGGARIQSLHRYGYPIGRLFATLAYGISARIIANMAFGRKAG
ELRDWGLKEALENTPKHAALNTLWTLTTVDLRDIAREIEKPTLIVVGKEDALLPVSKSEELSRLIKNSKMVIVPDAGHCV
MLEQPEIVNRVLEEFIHTFSAMLIRA

Sequences:

>Translated_266_residues
MPYADNGVKIYYEVEDGGEPAIVFVHGWTANMNFWREQREYFKGKHRMLFIDNRGHGKSDKPFNRSFYEFDNFVSDLHAA
VKDASFDRFVLVGHSFGTMISMRYCVEHPGRVEALVLIGGGARIQSLHRYGYPIGRLFATLAYGISARIIANMAFGRKAG
ELRDWGLKEALENTPKHAALNTLWTLTTVDLRDIAREIEKPTLIVVGKEDALLPVSKSEELSRLIKNSKMVIVPDAGHCV
MLEQPEIVNRVLEEFIHTFSAMLIRA
>Mature_265_residues
PYADNGVKIYYEVEDGGEPAIVFVHGWTANMNFWREQREYFKGKHRMLFIDNRGHGKSDKPFNRSFYEFDNFVSDLHAAV
KDASFDRFVLVGHSFGTMISMRYCVEHPGRVEALVLIGGGARIQSLHRYGYPIGRLFATLAYGISARIIANMAFGRKAGE
LRDWGLKEALENTPKHAALNTLWTLTTVDLRDIAREIEKPTLIVVGKEDALLPVSKSEELSRLIKNSKMVIVPDAGHCVM
LEQPEIVNRVLEEFIHTFSAMLIRA

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=280, Percent_Identity=23.9285714285714, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI218777840, Length=275, Percent_Identity=22.5454545454545, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI13376164, Length=275, Percent_Identity=22.5454545454545, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1787244, Length=241, Percent_Identity=24.4813278008299, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789817, Length=266, Percent_Identity=24.4360902255639, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554012, Length=274, Percent_Identity=20.4379562043796, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.1

Molecular weight: Translated: 30176; Mature: 30045

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYADNGVKIYYEVEDGGEPAIVFVHGWTANMNFWREQREYFKGKHRMLFIDNRGHGKSD
CCCCCCCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
KPFNRSFYEFDNFVSDLHAAVKDASFDRFVLVGHSFGTMISMRYCVEHPGRVEALVLIGG
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECC
GARIQSLHRYGYPIGRLFATLAYGISARIIANMAFGRKAGELRDWGLKEALENTPKHAAL
CHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCHHHH
NTLWTLTTVDLRDIAREIEKPTLIVVGKEDALLPVSKSEELSRLIKNSKMVIVPDAGHCV
HHHHEEEEECHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHHHHCCCEEEEECCCCEE
MLEQPEIVNRVLEEFIHTFSAMLIRA
EECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PYADNGVKIYYEVEDGGEPAIVFVHGWTANMNFWREQREYFKGKHRMLFIDNRGHGKSD
CCCCCCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCC
KPFNRSFYEFDNFVSDLHAAVKDASFDRFVLVGHSFGTMISMRYCVEHPGRVEALVLIGG
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECC
GARIQSLHRYGYPIGRLFATLAYGISARIIANMAFGRKAGELRDWGLKEALENTPKHAAL
CHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCHHHH
NTLWTLTTVDLRDIAREIEKPTLIVVGKEDALLPVSKSEELSRLIKNSKMVIVPDAGHCV
HHHHEEEEECHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHHHHCCCEEEEECCCCEE
MLEQPEIVNRVLEEFIHTFSAMLIRA
EECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carboxylic ester; H2O

Specific reaction: A carboxylic ester + H2O = an alcohol + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]