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The map label for this gene is braD-2

Identifier: 11498431

GI number: 11498431

Start: 735180

End: 736073

Strand: Reverse

Name: braD-2

Synonym: AF0825

Alternate gene names: 11498431

Gene position: 736073-735180 (Counterclockwise)

Preceding gene: 11498432

Following gene: 11498430

Centisome position: 33.79

GC content: 48.99

Gene sequence:

>894_bases
ATGATGATTGAAATAAATTATTTCATGAACCTGCTCTTAGGGGGACTCGTAGTGGGGTTCATTTATGCCCTAACGGCACT
GGGGATTACAATAATCTACGGTATTTTGCAGATTCCGGATTTCTCCCACGGGAACAGATACATGATCGGTGCTTACGCGG
GATTCCTTGCAGCCTCAGTAGCTGGCAGCATGCTTGAAATCAGCTATGTTGCTGCAATTCTCGGTGCAGTAGCAGTCTCT
TCCGTAATTGCCGTTCTGAGCTACCTTTTCGTTTACAAGAGATTGCTAAGTGCTCCTCACATAACGTCATTCATTGCAGC
AATAGGTCTTTTAATGCTGCTGGAAGGATTCGCCCTGGCAGTCTTCGGTCCGAGATACCAGCGCATTCACACACCCTTCG
ACTACATGGTTGTTATTGGAGCACTGAGAATTAACGCGCAGAGGCTTATCGTCCTTTTCTGCTCCATCGCCGCCATAGCG
TCTCTGCTGTACTTTTTGAGAAGGACGGTAACCGGAATAGCTCTCGAGGCTGTTGCTCAATCGCCAATAGGAGCGAAGCT
CGTTGGAATCAACACCGATAAGATGACGCTAATAGCCTTTGCCATTGCCGGGGCGCTTGCAGGTTTCGCAGCGGCATTAA
TTGCTCCAATAACTCTTGTGTATCCTATAATGGGTGCTGAGCTCAACTTGAAGGCCTTCGTAATCTGCGTCGTTGGTGGG
CTTGGGAACATCCCGGGTGCCATAGCCGGAGGGCTGGTCCTCGGAATTGTTGAATCCCTCTACGGCGGTTATTTTGACAT
CAGGTGGAAAAACCTCGTTGCGTTTGGGATTCTTGTCGCCATCCTGATTCTCAAGCCAGAAGGTCTGTTTGGTAAGAAGG
AGAGGAGAGCATGA

Upstream 100 bases:

>100_bases
TGAGGAAAAATTTCACTTTCTATGATGTACAGTGGGAGTCTAATGCTATTAATTGGAAAAAAACCAAAAAGGTTTTATAC
TTTTGCAATCCTATTTTAAA

Downstream 100 bases:

>100_bases
ACTGGAAGTTTGTTTATCCCGTAATTTTGATTCTGGCAGTGCTTCTGCCGCTGACAGGTTCGTCTTATGCGCTCAGGATT
TCAACGCTGTTCATAATATA

Product: branched-chain amino acid ABC transporter, permease protein (braD-2)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein H [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMIEINYFMNLLLGGLVVGFIYALTALGITIIYGILQIPDFSHGNRYMIGAYAGFLAASVAGSMLEISYVAAILGAVAVS
SVIAVLSYLFVYKRLLSAPHITSFIAAIGLLMLLEGFALAVFGPRYQRIHTPFDYMVVIGALRINAQRLIVLFCSIAAIA
SLLYFLRRTVTGIALEAVAQSPIGAKLVGINTDKMTLIAFAIAGALAGFAAALIAPITLVYPIMGAELNLKAFVICVVGG
LGNIPGAIAGGLVLGIVESLYGGYFDIRWKNLVAFGILVAILILKPEGLFGKKERRA

Sequences:

>Translated_297_residues
MMIEINYFMNLLLGGLVVGFIYALTALGITIIYGILQIPDFSHGNRYMIGAYAGFLAASVAGSMLEISYVAAILGAVAVS
SVIAVLSYLFVYKRLLSAPHITSFIAAIGLLMLLEGFALAVFGPRYQRIHTPFDYMVVIGALRINAQRLIVLFCSIAAIA
SLLYFLRRTVTGIALEAVAQSPIGAKLVGINTDKMTLIAFAIAGALAGFAAALIAPITLVYPIMGAELNLKAFVICVVGG
LGNIPGAIAGGLVLGIVESLYGGYFDIRWKNLVAFGILVAILILKPEGLFGKKERRA
>Mature_297_residues
MMIEINYFMNLLLGGLVVGFIYALTALGITIIYGILQIPDFSHGNRYMIGAYAGFLAASVAGSMLEISYVAAILGAVAVS
SVIAVLSYLFVYKRLLSAPHITSFIAAIGLLMLLEGFALAVFGPRYQRIHTPFDYMVVIGALRINAQRLIVLFCSIAAIA
SLLYFLRRTVTGIALEAVAQSPIGAKLVGINTDKMTLIAFAIAGALAGFAAALIAPITLVYPIMGAELNLKAFVICVVGG
LGNIPGAIAGGLVLGIVESLYGGYFDIRWKNLVAFGILVAILILKPEGLFGKKERRA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=298, Percent_Identity=30.5369127516779, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31498; Mature: 31498

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIEINYFMNLLLGGLVVGFIYALTALGITIIYGILQIPDFSHGNRYMIGAYAGFLAASV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
AGSMLEISYVAAILGAVAVSSVIAVLSYLFVYKRLLSAPHITSFIAAIGLLMLLEGFALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFGPRYQRIHTPFDYMVVIGALRINAQRLIVLFCSIAAIASLLYFLRRTVTGIALEAVAQ
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPIGAKLVGINTDKMTLIAFAIAGALAGFAAALIAPITLVYPIMGAELNLKAFVICVVGG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
LGNIPGAIAGGLVLGIVESLYGGYFDIRWKNLVAFGILVAILILKPEGLFGKKERRA
CCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MMIEINYFMNLLLGGLVVGFIYALTALGITIIYGILQIPDFSHGNRYMIGAYAGFLAASV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
AGSMLEISYVAAILGAVAVSSVIAVLSYLFVYKRLLSAPHITSFIAAIGLLMLLEGFALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFGPRYQRIHTPFDYMVVIGALRINAQRLIVLFCSIAAIASLLYFLRRTVTGIALEAVAQ
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPIGAKLVGINTDKMTLIAFAIAGALAGFAAALIAPITLVYPIMGAELNLKAFVICVVGG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
LGNIPGAIAGGLVLGIVESLYGGYFDIRWKNLVAFGILVAILILKPEGLFGKKERRA
CCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]