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The map label for this gene is braE-2

Identifier: 11498430

GI number: 11498430

Start: 734212

End: 735183

Strand: Reverse

Name: braE-2

Synonym: AF0824

Alternate gene names: 11498430

Gene position: 735183-734212 (Counterclockwise)

Preceding gene: 11498431

Following gene: 11498429

Centisome position: 33.75

GC content: 47.94

Gene sequence:

>972_bases
ATGAACTGGAAGTTTGTTTATCCCGTAATTTTGATTCTGGCAGTGCTTCTGCCGCTGACAGGTTCGTCTTATGCGCTCAG
GATTTCAACGCTGTTCATAATATACACAATAGCTGCCATGGGGCTGAACATAATAATCGGATACACAGGCCAAGCTTCTC
TGGCTCAGGGAGCTTTTTACGGTCTTGGAGCCTACGTTAGCGCCTTGCTTGTGCTTAACGGCTGGTCTTTCTGGGCGTCA
GTGATCGTGGCAATCGCCTTCGTGGCCTTTGTGGCGTTCCTGATAGGTCTAATGACTCTCAGACTTAAGGGTGCGTACTT
TGCCATTGCCACGCTCTTATTCAACGTCATAATCTATGAAGTTGTGGACAAGTGGGATGAGGTTACCAGAGGGCCGAGGG
GGCTTTTTGGCATCCCTTCTCCGTCAATTGAGCTTAGCGGTATTTCAATCAGTTTCAGTGGAACCTACAACTTTTACTAC
CTCATGCTGGCCGTTCTGATCGTCATGACTCTCCTTTACTGGCTTATAATCAGGTCCTCCTTCGGCAGCATAATAGTTGC
AATCAGAGAGAATGAGCTATTGGCCGAGTACGCAGGGGTAAATCTTACGAAGTTCAAGGTTGCCTCTTTCGTCTTAAGTG
CCATCTTCGCCGCTGTTGCTGGAGCCTTCTACTCTGCATACATCGGCAGCATCTTTCCAGATATTACGAGCTTCGTTAAC
AGCTTTGCATTCCTCACCGCTATCATCTTTGGAGGCGCTGGAACTATGCTTGGACCGTTCATAGGAACCGCCGTTGTTAC
GCTGATAAACGAGGTCTTCTTCACACTCGCTCAGTACACAGTCCTCGTTCAGGGAATCGTATTCCTTTTGGCGATTCTGT
TCATGCCAAAAGGAGTTATGGGGGCTTACTACTCTAGAAAGGCCGAGAAGGGAGAAAAACTTCCCAAAAGGGCTCTTGTA
GGTAAGTTCTGA

Upstream 100 bases:

>100_bases
ATTTTGACATCAGGTGGAAAAACCTCGTTGCGTTTGGGATTCTTGTCGCCATCCTGATTCTCAAGCCAGAAGGTCTGTTT
GGTAAGAAGGAGAGGAGAGC

Downstream 100 bases:

>100_bases
TAGGTGGTAGAATGCTGAAAGTTGAGGGAGTTGGAATAAGATTTGGAGGTCTAATAGCTCTGAACAACGTAACCTTTGAA
ATCAGGAAGGGGGAAGTTCT

Product: branched-chain amino acid ABC transporter, permease protein (braE-2)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNWKFVYPVILILAVLLPLTGSSYALRISTLFIIYTIAAMGLNIIIGYTGQASLAQGAFYGLGAYVSALLVLNGWSFWAS
VIVAIAFVAFVAFLIGLMTLRLKGAYFAIATLLFNVIIYEVVDKWDEVTRGPRGLFGIPSPSIELSGISISFSGTYNFYY
LMLAVLIVMTLLYWLIIRSSFGSIIVAIRENELLAEYAGVNLTKFKVASFVLSAIFAAVAGAFYSAYIGSIFPDITSFVN
SFAFLTAIIFGGAGTMLGPFIGTAVVTLINEVFFTLAQYTVLVQGIVFLLAILFMPKGVMGAYYSRKAEKGEKLPKRALV
GKF

Sequences:

>Translated_323_residues
MNWKFVYPVILILAVLLPLTGSSYALRISTLFIIYTIAAMGLNIIIGYTGQASLAQGAFYGLGAYVSALLVLNGWSFWAS
VIVAIAFVAFVAFLIGLMTLRLKGAYFAIATLLFNVIIYEVVDKWDEVTRGPRGLFGIPSPSIELSGISISFSGTYNFYY
LMLAVLIVMTLLYWLIIRSSFGSIIVAIRENELLAEYAGVNLTKFKVASFVLSAIFAAVAGAFYSAYIGSIFPDITSFVN
SFAFLTAIIFGGAGTMLGPFIGTAVVTLINEVFFTLAQYTVLVQGIVFLLAILFMPKGVMGAYYSRKAEKGEKLPKRALV
GKF
>Mature_323_residues
MNWKFVYPVILILAVLLPLTGSSYALRISTLFIIYTIAAMGLNIIIGYTGQASLAQGAFYGLGAYVSALLVLNGWSFWAS
VIVAIAFVAFVAFLIGLMTLRLKGAYFAIATLLFNVIIYEVVDKWDEVTRGPRGLFGIPSPSIELSGISISFSGTYNFYY
LMLAVLIVMTLLYWLIIRSSFGSIIVAIRENELLAEYAGVNLTKFKVASFVLSAIFAAVAGAFYSAYIGSIFPDITSFVN
SFAFLTAIIFGGAGTMLGPFIGTAVVTLINEVFFTLAQYTVLVQGIVFLLAILFMPKGVMGAYYSRKAEKGEKLPKRALV
GKF

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=321, Percent_Identity=29.2834890965732, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35118; Mature: 35118

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKFVYPVILILAVLLPLTGSSYALRISTLFIIYTIAAMGLNIIIGYTGQASLAQGAFY
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
GLGAYVSALLVLNGWSFWASVIVAIAFVAFVAFLIGLMTLRLKGAYFAIATLLFNVIIYE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDKWDEVTRGPRGLFGIPSPSIELSGISISFSGTYNFYYLMLAVLIVMTLLYWLIIRSS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
FGSIIVAIRENELLAEYAGVNLTKFKVASFVLSAIFAAVAGAFYSAYIGSIFPDITSFVN
CCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFAFLTAIIFGGAGTMLGPFIGTAVVTLINEVFFTLAQYTVLVQGIVFLLAILFMPKGVM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GAYYSRKAEKGEKLPKRALVGKF
HHHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure
MNWKFVYPVILILAVLLPLTGSSYALRISTLFIIYTIAAMGLNIIIGYTGQASLAQGAFY
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
GLGAYVSALLVLNGWSFWASVIVAIAFVAFVAFLIGLMTLRLKGAYFAIATLLFNVIIYE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDKWDEVTRGPRGLFGIPSPSIELSGISISFSGTYNFYYLMLAVLIVMTLLYWLIIRSS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
FGSIIVAIRENELLAEYAGVNLTKFKVASFVLSAIFAAVAGAFYSAYIGSIFPDITSFVN
CCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFAFLTAIIFGGAGTMLGPFIGTAVVTLINEVFFTLAQYTVLVQGIVFLLAILFMPKGVM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GAYYSRKAEKGEKLPKRALVGKF
HHHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]