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The map label for this gene is alkK-3

Identifier: 11498280

GI number: 11498280

Start: 605326

End: 606840

Strand: Reverse

Name: alkK-3

Synonym: AF0672

Alternate gene names: 11498280

Gene position: 606840-605326 (Counterclockwise)

Preceding gene: 11498281

Following gene: 11498279

Centisome position: 27.86

GC content: 48.91

Gene sequence:

>1515_bases
ATGGCTTATTTTTGTTTGGGTGAGAGTGTGTTTACTATACCGTATCTTCTGGAAAGAGCGGCAAAGCTTTTTCCCAACAA
GGGAGTTGTTGGCAGGGAAGGGACAAAAAGCTACTCAGAAGTAAGGAAAAGGGTTCTTGGGCTAACAGCGTTTTTTGAGA
ACATAGGGATACGGAACGAGGTTGTGGCAGTAGCGGACTGGAACACACCGGAGTTCTTCGAGCTGATTTATGCGATAACT
GCAGCTGGAGGGATTCTGTATCCGGTCAACATAAGACTGCCACCAGATCAAATAGCCTACACGCTGAAAAAATCGGAATC
GGGACTTCTGATTTACTCCGACGACTTTTCAGCCCTTGCAAAAGTTCATGAAGGAGAAAGCTTGCACATAAAAGAGGTGG
CTGAGAAGGCTGATGAGGCAGAGCCTGAAGTCAACGTTAAGCAGGATGATATGGCTGTTATGCTCTTTACCAGCGGTACC
ACGGGATTGCCAAAGGCTGTGAGATACACTCACGAAAAAATGATTCACGGAGCTCTTAGCATAGCACATCAGCTTGCTCA
CCACGAGACTCCTGCAAATCTAACTCCGAGGGACGTAATCTTCCCTCAGATACCCATCTACCACATTCTCGCTTGGGGAA
CTGTGTTCATAGCCCCCTACATGGGTTTGAATCTGGTGATGGGCGGAAGATTTGATCCGGCAGAGGCTGTGAGGCTCATC
AAAAAAGAGGGGGTAACATGGATTAACGCCGTTCCGACAATGGTTAACATGCTCCTCGAGACGAAAGAGGACCTCAGCGG
CATTAAGGTGCTCATCGGAGGGAGTACGATTACAATTGACCTGGCAAGGCGCATGGAAAAAGCAGGAATGAAGTTCTCAA
CAATCTATGGCGGTACCGACATGCTGGCTGCATCCATTGCTATTATGACCGAAGAGGCCAAGGTGAAGGGTGTTGACTAC
ATCAGGCAGGTAACTCATCCCGTTCCCTTTGCGGAGTTCAAAATAGTCCCCCAGGAGGGAATGGGGGGTGAGTCGGGGGA
GATATACTTCAGAGCCCCTTGGCTACCCATGGAGTACTACAAGGACGAGGAGAAAACCAAAGAAGCCTACACCGAAGATG
GCTGGTTCAAAACAGGAGACCTCGGAGTTGAGATGCCAGACGGAGGAGTAAAGATACTCGACAGGGTGAAGGATGCCATC
AAATCCGGCGGAGAGTGGATTCCGAGCAGCATTCTCGAATCAGTGATTTCGGAAATTGAGTGGGTGGAGTACGTTGCAGT
TCTCGGCAAGCCCGACGAGAAGTGGGGTGAGAGACCTTTTGCAGTAATAAAGCCCGTTGACAGGAGTAAGGCGAGCATTG
AAGAGGTGAAAGAGCATCTGGCCAAAGCTGTTGAAGAGGGCAGAATTGCCAAGTGGTGGATTCCGGAGGAAATTGCGATT
GTTGATGATATGCCTCTCACGAGCACGGGAAAAATTAACAAACTTTCGTTAAGAAAAATGCTGTTGGAGAGGTGA

Upstream 100 bases:

>100_bases
TCTAAAAGAGGACCTTCTCAACTTAAGGGAGATGCTAGTCAAGAGACTTGAGGAGCTGGAAAAGGGAAGTTAACGTAAAC
GTTAAATTTAAATATTATTT

Downstream 100 bases:

>100_bases
TGTAAATGCTCGAAAACGACGCCCTTCTTACTGAAGAGGAGATTAAGATAAGGGAGGAGGCAAAGGAGCTTGTTAATGCT
ATTGACCCCGAGCTTCTGAG

Product: medium-chain acyl-CoA ligase (alkK-3)

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAYFCLGESVFTIPYLLERAAKLFPNKGVVGREGTKSYSEVRKRVLGLTAFFENIGIRNEVVAVADWNTPEFFELIYAIT
AAGGILYPVNIRLPPDQIAYTLKKSESGLLIYSDDFSALAKVHEGESLHIKEVAEKADEAEPEVNVKQDDMAVMLFTSGT
TGLPKAVRYTHEKMIHGALSIAHQLAHHETPANLTPRDVIFPQIPIYHILAWGTVFIAPYMGLNLVMGGRFDPAEAVRLI
KKEGVTWINAVPTMVNMLLETKEDLSGIKVLIGGSTITIDLARRMEKAGMKFSTIYGGTDMLAASIAIMTEEAKVKGVDY
IRQVTHPVPFAEFKIVPQEGMGGESGEIYFRAPWLPMEYYKDEEKTKEAYTEDGWFKTGDLGVEMPDGGVKILDRVKDAI
KSGGEWIPSSILESVISEIEWVEYVAVLGKPDEKWGERPFAVIKPVDRSKASIEEVKEHLAKAVEEGRIAKWWIPEEIAI
VDDMPLTSTGKINKLSLRKMLLER

Sequences:

>Translated_504_residues
MAYFCLGESVFTIPYLLERAAKLFPNKGVVGREGTKSYSEVRKRVLGLTAFFENIGIRNEVVAVADWNTPEFFELIYAIT
AAGGILYPVNIRLPPDQIAYTLKKSESGLLIYSDDFSALAKVHEGESLHIKEVAEKADEAEPEVNVKQDDMAVMLFTSGT
TGLPKAVRYTHEKMIHGALSIAHQLAHHETPANLTPRDVIFPQIPIYHILAWGTVFIAPYMGLNLVMGGRFDPAEAVRLI
KKEGVTWINAVPTMVNMLLETKEDLSGIKVLIGGSTITIDLARRMEKAGMKFSTIYGGTDMLAASIAIMTEEAKVKGVDY
IRQVTHPVPFAEFKIVPQEGMGGESGEIYFRAPWLPMEYYKDEEKTKEAYTEDGWFKTGDLGVEMPDGGVKILDRVKDAI
KSGGEWIPSSILESVISEIEWVEYVAVLGKPDEKWGERPFAVIKPVDRSKASIEEVKEHLAKAVEEGRIAKWWIPEEIAI
VDDMPLTSTGKINKLSLRKMLLER
>Mature_503_residues
AYFCLGESVFTIPYLLERAAKLFPNKGVVGREGTKSYSEVRKRVLGLTAFFENIGIRNEVVAVADWNTPEFFELIYAITA
AGGILYPVNIRLPPDQIAYTLKKSESGLLIYSDDFSALAKVHEGESLHIKEVAEKADEAEPEVNVKQDDMAVMLFTSGTT
GLPKAVRYTHEKMIHGALSIAHQLAHHETPANLTPRDVIFPQIPIYHILAWGTVFIAPYMGLNLVMGGRFDPAEAVRLIK
KEGVTWINAVPTMVNMLLETKEDLSGIKVLIGGSTITIDLARRMEKAGMKFSTIYGGTDMLAASIAIMTEEAKVKGVDYI
RQVTHPVPFAEFKIVPQEGMGGESGEIYFRAPWLPMEYYKDEEKTKEAYTEDGWFKTGDLGVEMPDGGVKILDRVKDAIK
SGGEWIPSSILESVISEIEWVEYVAVLGKPDEKWGERPFAVIKPVDRSKASIEEVKEHLAKAVEEGRIAKWWIPEEIAIV
DDMPLTSTGKINKLSLRKMLLER

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=361, Percent_Identity=29.9168975069252, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI187761345, Length=550, Percent_Identity=25.6363636363636, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI187761343, Length=550, Percent_Identity=25.6363636363636, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI42544132, Length=470, Percent_Identity=25.3191489361702, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI38505220, Length=467, Percent_Identity=23.5546038543897, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI28416953, Length=427, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI145693145, Length=392, Percent_Identity=29.8469387755102, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=23.9299610894942, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788595, Length=433, Percent_Identity=27.2517321016166, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786810, Length=373, Percent_Identity=26.2734584450402, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788107, Length=362, Percent_Identity=27.0718232044199, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786801, Length=443, Percent_Identity=23.7020316027088, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=409, Percent_Identity=27.1393643031785, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=450, Percent_Identity=28.8888888888889, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=27.4193548387097, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=27.4193548387097, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=372, Percent_Identity=27.4193548387097, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=481, Percent_Identity=25.7796257796258, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=505, Percent_Identity=24.950495049505, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=22.9126213592233, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=515, Percent_Identity=25.631067961165, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=497, Percent_Identity=24.3460764587525, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.4660194174757, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=381, Percent_Identity=25.7217847769029, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=476, Percent_Identity=21.4285714285714, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=453, Percent_Identity=23.6203090507726, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556552, Length=91, Percent_Identity=37.3626373626374, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323903, Length=374, Percent_Identity=27.0053475935829, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319699, Length=356, Percent_Identity=24.438202247191, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324893, Length=432, Percent_Identity=23.8425925925926, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=435, Percent_Identity=24.367816091954, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322182, Length=332, Percent_Identity=24.0963855421687, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323182, Length=409, Percent_Identity=25.1833740831296, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859661, Length=492, Percent_Identity=27.6422764227642, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581924, Length=402, Percent_Identity=28.1094527363184, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355181, Length=504, Percent_Identity=24.6031746031746, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648253, Length=520, Percent_Identity=23.4615384615385, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653035, Length=376, Percent_Identity=27.6595744680851, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356441, Length=501, Percent_Identity=23.75249500998, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648255, Length=500, Percent_Identity=23.8, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076582, Length=384, Percent_Identity=27.8645833333333, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356947, Length=360, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358303, Length=333, Percent_Identity=27.3273273273273, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=491, Percent_Identity=23.2179226069246, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=25.4054054054054, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648260, Length=374, Percent_Identity=24.0641711229947, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281365686, Length=375, Percent_Identity=23.2, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 56155; Mature: 56023

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYFCLGESVFTIPYLLERAAKLFPNKGVVGREGTKSYSEVRKRVLGLTAFFENIGIRNE
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VVAVADWNTPEFFELIYAITAAGGILYPVNIRLPPDQIAYTLKKSESGLLIYSDDFSALA
EEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHEEEEECCCCEEEECCCHHHHH
KVHEGESLHIKEVAEKADEAEPEVNVKQDDMAVMLFTSGTTGLPKAVRYTHEKMIHGALS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IAHQLAHHETPANLTPRDVIFPQIPIYHILAWGTVFIAPYMGLNLVMGGRFDPAEAVRLI
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
KKEGVTWINAVPTMVNMLLETKEDLSGIKVLIGGSTITIDLARRMEKAGMKFSTIYGGTD
HHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEECCHH
MLAASIAIMTEEAKVKGVDYIRQVTHPVPFAEFKIVPQEGMGGESGEIYFRAPWLPMEYY
HHHHHHHEEECCHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCEEEEECCCCCHHHH
KDEEKTKEAYTEDGWFKTGDLGVEMPDGGVKILDRVKDAIKSGGEWIPSSILESVISEIE
CCHHHHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
WVEYVAVLGKPDEKWGERPFAVIKPVDRSKASIEEVKEHLAKAVEEGRIAKWWIPEEIAI
HHHHHHHHCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEE
VDDMPLTSTGKINKLSLRKMLLER
ECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AYFCLGESVFTIPYLLERAAKLFPNKGVVGREGTKSYSEVRKRVLGLTAFFENIGIRNE
EEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VVAVADWNTPEFFELIYAITAAGGILYPVNIRLPPDQIAYTLKKSESGLLIYSDDFSALA
EEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCHHHHHEEEEECCCCEEEECCCHHHHH
KVHEGESLHIKEVAEKADEAEPEVNVKQDDMAVMLFTSGTTGLPKAVRYTHEKMIHGALS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IAHQLAHHETPANLTPRDVIFPQIPIYHILAWGTVFIAPYMGLNLVMGGRFDPAEAVRLI
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
KKEGVTWINAVPTMVNMLLETKEDLSGIKVLIGGSTITIDLARRMEKAGMKFSTIYGGTD
HHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCEEEEEECCHH
MLAASIAIMTEEAKVKGVDYIRQVTHPVPFAEFKIVPQEGMGGESGEIYFRAPWLPMEYY
HHHHHHHEEECCHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCEEEEECCCCCHHHH
KDEEKTKEAYTEDGWFKTGDLGVEMPDGGVKILDRVKDAIKSGGEWIPSSILESVISEIE
CCHHHHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
WVEYVAVLGKPDEKWGERPFAVIKPVDRSKASIEEVKEHLAKAVEEGRIAKWWIPEEIAI
HHHHHHHHCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEE
VDDMPLTSTGKINKLSLRKMLLER
ECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]