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The map label for this gene is leuD

Identifier: 11498237

GI number: 11498237

Start: 566165

End: 566650

Strand: Reverse

Name: leuD

Synonym: AF0629

Alternate gene names: 11498237

Gene position: 566650-566165 (Counterclockwise)

Preceding gene: 11498238

Following gene: 11498236

Centisome position: 26.01

GC content: 49.18

Gene sequence:

>486_bases
ATGGGAAGAGCCTGGAAGTTCGGAGATGACATAGACACGGACGTCATCATACAGGGAAAGTATCTGGTAATCAATGAGCC
TGAGGAGCTCGCAAAGCATGTTTTTGAGAATCTTCGCCCCGAATTCGCCAAGGAGGTTAAGAAGGGAGATTTCGTTGTTG
CTGGAGAGAATTTCGGCTGTGGGAGCAGCAGAGAGCACGCCCCTCTCGCTTTGAAGGCTACGGGAATTGAGGCTGTCATT
GCAAAATCCTACGCCAGAATTTTCTTCAGGAATGCCATCAACATCGGTCTGAGAGTTCTTGAGTGCAAGGAGACGGACAA
GATTGAAGACGGAGACGAGCTGGAAGTTGATTACGAGAAGGGCGTGATTTACAACAAAACAAAGGGTGAGGAGTATCCAA
TCAACCCACTGCCCGATTTCCTGAAGGAGATTCTGGAAAAGGGCGGGCTGGTTGAGTTTGCAAAGAGTTTGAGGGAGAGG
GCATGA

Upstream 100 bases:

>100_bases
AGGATGTTTCTCGCCGGTGTTGTGACTGCTGTGATAGTAACAATTCTCAGCCCCAGCCACTTCATCTGAAACCTTTTTTA
ATTCCGCCCCTACTTAGCTC

Downstream 100 bases:

>100_bases
AGAAAATTGTGGTAATCCCCGGAGACGGCATAGGCAAGGAGGTTATGGAGGCTGCGATGCTAATTCTCGAGAAGCTCGAC
CTGCCCTTTGAATACAGCTA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MGRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGCGSSREHAPLALKATGIEAVI
AKSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEKGVIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAKSLRER
A

Sequences:

>Translated_161_residues
MGRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGCGSSREHAPLALKATGIEAVI
AKSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEKGVIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAKSLRER
A
>Mature_160_residues
GRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGCGSSREHAPLALKATGIEAVIA
KSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEKGVIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAKSLRERA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=131, Percent_Identity=32.824427480916, Blast_Score=67, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=46.875, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=105, Percent_Identity=40, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD1_ARCFU (O29626)

Other databases:

- EMBL:   AE000782
- PIR:   E69328
- RefSeq:   NP_069463.1
- ProteinModelPortal:   O29626
- SMR:   O29626
- GeneID:   1483847
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0629
- NMPDR:   fig|224325.1.peg.622
- TIGR:   AF_0629
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   PRK00439
- BioCyc:   AFUL224325:AF_0629-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18137; Mature: 18006

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGC
CCCCCCCCCCCCCCEEEECCEEEEECHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCC
GSSREHAPLALKATGIEAVIAKSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEK
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEECC
GVIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAKSLRERA
CEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GRAWKFGDDIDTDVIIQGKYLVINEPEELAKHVFENLRPEFAKEVKKGDFVVAGENFGC
CCCCCCCCCCCCCEEEECCEEEEECHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCC
GSSREHAPLALKATGIEAVIAKSYARIFFRNAINIGLRVLECKETDKIEDGDELEVDYEK
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEECC
GVIYNKTKGEEYPINPLPDFLKEILEKGGLVEFAKSLRERA
CEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475