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The map label for this gene is leuB

Identifier: 11498236

GI number: 11498236

Start: 565188

End: 566168

Strand: Reverse

Name: leuB

Synonym: AF0628

Alternate gene names: 11498236

Gene position: 566168-565188 (Counterclockwise)

Preceding gene: 11498237

Following gene: 11498235

Centisome position: 25.99

GC content: 51.07

Gene sequence:

>981_bases
ATGAAGAAAATTGTGGTAATCCCCGGAGACGGCATAGGCAAGGAGGTTATGGAGGCTGCGATGCTAATTCTCGAGAAGCT
CGACCTGCCCTTTGAATACAGCTACTACGATGCTGGAGATGAGGCTCTGGAGAAGTACGGAAAAGCTCTGCCTGATGAGA
CGCTGGAAGCTTGCAGGAAGAGCGATGCGGTGCTTTTCGGTGCCGCCGGGGAAACGGCGGCAGATGTCATAGTGAGGCTG
AGAAGGGAGCTCGGAACCTTTGCCAACGTCAGGCCAGCGAAGGCAATTGAGGGCATTGAATGCCTCTATCCCGGCTTAGA
CATTGTTGTGGTGAGGGAAAACACAGAGTGCCTTTACATGGGCTTCGAGTTCGGCTTCGGGGACGTTACTGAAGCCATCA
GAGTGATTACGAGAGAGGCTTCCGAGAGAATTGCGAGGTATGCTTTTGAGCTGGCTAAAAGGGAGGGCAGGAAGAAGGTT
ACAGCCCTGCACAAAGCCAATGTTATGAAGAAAACATGCGGGCTATTTAGGGATGTGTGCAGAGAAGTTGCGAAAGATTA
TCCCGAAATTCAGTACAATGACTACTACATCGATGCAGCTTGCATGTACCTCGTTATGGACCCCTTCCGCTTCGATGTAA
TCGTTACGACAAACATGTTTGGTGATATCGTCTCTGACTTAGCTGCAGGCCTCGTTGGAGGACTTGGTCTTGCCCCCTCA
GCCAATGTTGGGGAGAGGACGGCCATTTTCGAGCCGGTACATGGTGCTGCCTTCGACATCGCTGGAAAGGGAATTGCAAA
TCCAACTGCCATGATTCTCACAGCCTGCATGATGCTCCGCCACTTCGGCTATGTTGAGGAGGCCAAGAAGGTTGAGGAAG
CTGTTGAGAAGACCATTAAGGAGGGGAAGAAGACTCCGGATTTGGGAGGTAACCTCAAAACTATGGAGTTTGCCAACGAA
GTTGCATCCCTTTTGGATTAG

Upstream 100 bases:

>100_bases
AGGGTGAGGAGTATCCAATCAACCCACTGCCCGATTTCCTGAAGGAGATTCTGGAAAAGGGCGGGCTGGTTGAGTTTGCA
AAGAGTTTGAGGGAGAGGGC

Downstream 100 bases:

>100_bases
ATTTAAATAGTAAAACTTGACTAATTTTTTTCATGAGCTACGAAATATTGATGGCAAGGTTGGGCTTTCCCGACTCGGAA
AGGCTCAGGAGGATACTGGA

Product: multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRKSDAVLFGAAGETAADVIVRL
RRELGTFANVRPAKAIEGIECLYPGLDIVVVRENTECLYMGFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKV
TALHKANVMKKTCGLFRDVCREVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGLGLAPS
ANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAVEKTIKEGKKTPDLGGNLKTMEFANE
VASLLD

Sequences:

>Translated_326_residues
MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRKSDAVLFGAAGETAADVIVRL
RRELGTFANVRPAKAIEGIECLYPGLDIVVVRENTECLYMGFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKV
TALHKANVMKKTCGLFRDVCREVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGLGLAPS
ANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAVEKTIKEGKKTPDLGGNLKTMEFANE
VASLLD
>Mature_326_residues
MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRKSDAVLFGAAGETAADVIVRL
RRELGTFANVRPAKAIEGIECLYPGLDIVVVRENTECLYMGFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKV
TALHKANVMKKTCGLFRDVCREVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGLGLAPS
ANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAVEKTIKEGKKTPDLGGNLKTMEFANE
VASLLD

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

Homologues:

Organism=Homo sapiens, GI5031777, Length=332, Percent_Identity=44.578313253012, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI4758582, Length=328, Percent_Identity=38.719512195122, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI28178821, Length=333, Percent_Identity=36.9369369369369, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI28178816, Length=332, Percent_Identity=36.7469879518072, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI28178838, Length=301, Percent_Identity=39.202657807309, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI28178819, Length=200, Percent_Identity=42.5, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=38.3098591549296, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI87081683, Length=336, Percent_Identity=38.6904761904762, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=30.6010928961749, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=41.0334346504559, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17550882, Length=333, Percent_Identity=41.7417417417417, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=38.5294117647059, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=37.2727272727273, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=44.5402298850575, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6324291, Length=332, Percent_Identity=39.7590361445783, Blast_Score=239, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324709, Length=325, Percent_Identity=39.6923076923077, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=36.3380281690141, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=44.9101796407186, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=44.9101796407186, Blast_Score=280, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24661184, Length=328, Percent_Identity=40.5487804878049, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI161078637, Length=330, Percent_Identity=37.5757575757576, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078635, Length=330, Percent_Identity=37.5757575757576, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078633, Length=330, Percent_Identity=37.5757575757576, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24650122, Length=330, Percent_Identity=37.5757575757576, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078639, Length=328, Percent_Identity=37.8048780487805, Blast_Score=217, Evalue=8e-57,
Organism=Drosophila melanogaster, GI281362242, Length=326, Percent_Identity=33.1288343558282, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24648872, Length=326, Percent_Identity=33.1288343558282, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI20130355, Length=326, Percent_Identity=30.9815950920245, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45550436, Length=222, Percent_Identity=23.8738738738739, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_ARCFU (O29627)

Other databases:

- EMBL:   AE000782
- PIR:   D69328
- RefSeq:   NP_069462.1
- ProteinModelPortal:   O29627
- SMR:   O29627
- GeneID:   1483846
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0628
- NMPDR:   fig|224325.1.peg.621
- TIGR:   AF_0628
- HOGENOM:   HBG518924
- OMA:   TCAHKAN
- ProtClustDB:   PRK14025
- BioCyc:   AFUL224325:AF_0628-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- TIGRFAMs:   TIGR02088

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 35820; Mature: 35820

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 81-81 BINDING 91-91 BINDING 112-112 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRK
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHC
SDAVLFGAAGETAADVIVRLRRELGTFANVRPAKAIEGIECLYPGLDIVVVRENTECLYM
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCEEEE
GFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKVTALHKANVMKKTCGLFRDVC
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGLGLAPS
HHHHHCCCCCCCCCEEHHHHHHHHHHCCHHEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
ANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAVEKTIK
CCCCCCHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EGKKTPDLGGNLKTMEFANEVASLLD
CCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVVIPGDGIGKEVMEAAMLILEKLDLPFEYSYYDAGDEALEKYGKALPDETLEACRK
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHC
SDAVLFGAAGETAADVIVRLRRELGTFANVRPAKAIEGIECLYPGLDIVVVRENTECLYM
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCEEEE
GFEFGFGDVTEAIRVITREASERIARYAFELAKREGRKKVTALHKANVMKKTCGLFRDVC
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGDIVSDLAAGLVGGLGLAPS
HHHHHCCCCCCCCCEEHHHHHHHHHHCCHHEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
ANVGERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRHFGYVEEAKKVEEAVEKTIK
CCCCCCHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EGKKTPDLGGNLKTMEFANEVASLLD
CCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475