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Identifier: 11498154

GI number: 11498154

Start: 494553

End: 496118

Strand: Reverse

Name: Not Available

Synonym: AF0544

Alternate gene names: NA

Gene position: 496118-494553 (Counterclockwise)

Preceding gene: 11498155

Following gene: 11498153

Centisome position: 22.77

GC content: 48.98

Gene sequence:

>1566_bases
ATGCCAGAATACATCTTCAACTTTATTTCTTTTGAGGAGTTTTGTGAGGTGGAAAAGAGGATTCTCAGGCTCAGGTTGAG
CGAACTTTCCCACGTTCCATTTTCAGAGGAGCCAGAGCTTCAGGAACCAAATCCTGAGTGGAGCGAGAAGTACGTTACTG
AGCTTGACGGCTACATAGCCATAGACGAGCCTTGGAAACCGCAGAATGAGGAAGAGGAAAAGGAGCTGGTTGAGAGGTTC
CTTTCCGGGCTGAGAAAGCTCCTTGACAGGGAGGCAAACTGGACATTCTTGCAGCCTTTAATGCTTTCTTTGGAGTACTG
CGCCCGGTGCCAGACCTGCAGTGAAGCCTGTCCCATTTACGTGTCAAGCGGGAGGAAGGAAATTTACAGCCCCCTCTTCA
GGTCAGAGATGTTCAGAAAAATCATCAGGGAAACAAAGGGCGGGAAGAGAAGGGGAAGGCTGACGGTAAAGGCCATTTTG
AGGCTCGCTGAACTCGCCTACAGATGCACCCTCTGCAGAAGATGCACCCTCGCATGTCCACTCGGAATAGACAACGGCCT
GATAACGAGAGAAATAAGGAAGCTCTTCAGCCAGGAGATGGGAATAGCTCCTTCATCCCTGCACATGCAGGGTAGTGTGC
AGCAGCTTGTTGATGGCACATCCGTTGGCTTCACACCCATCGTAATGGAGGATTTTGTCAGAGAGATAGAGTTCATGGTA
AGTCAGGTGCTTGGAAGGAGGTACTATCTTCCAGTGGACGTTAAAGGAGTTGACATCCTCCTGATTCACAGCGTTGCGGA
CTACATCCATCACCCTGAAACCATAGCCGCAATGGCAATCATTTTCGAAATGGCTGGCTATGAGTGGACGCTGAGCAGCA
AAGCTCCGGCCCTCGATGCGGTCAATTATGGAATGTGGTATGACGATATACAGCTTGCAAGAATAGCTCTGAGCCATGTT
AAAGTAGCGAGTGAGCTTGACGTTGAAAGGATTGTGGTTGGTGAGAGCGGGCATGCGCACAGCACGTTGCTGACGACTTC
TGAAAGGTTCTTCGTCGAGCTTTTTCCAGTTGAGAGCTATCTCCCACTTCTGTGGAAAATTGTCAGAAGGAGAGAAATCA
AATTTGACGCCGATTTAAACGACTTTCCAGTCACCCTTCATGACCCCTGCTACATAACGAGGTTTACAGGCATAATCAAG
CCACAGAGGGACATTCTGAAGGACGTCTGCCCCCAGTTCAGGGAGATGAAGCCAAGCGGCGTGAAAAACTACTGCTGCGG
CGGAGGAGGGGGGCTTGCAGTTATCAACACAATGAACTTTCCCGACTTCAGGGCGAGGATAGCGAGCAGGCTTAAAGTTA
AGCAGATTGTAGATGCCTTCAACTCTGACGTCAGCGAGAAAGTGCAGAAGTATGTCTGTGCTCCCTGCAGCAACTGCAAG
ATGACAATAAACCACGCCCTCAAGCATTTTGGCCTCAACAGGTTCAATATGAAGTGTGTGGGAATCCCTGAGATTGTTTT
GAACTGTGTTGAGAACGTTGAAAAGAAAATTCTCAGTATTGTTTAG

Upstream 100 bases:

>100_bases
GACCTGACATGGGCGGAAGAGGCTACAGCCATCGAACCTGTTCTTGAGGTCAGGGCTCTGGCCGAGAGAAGAAAGAGGAG
GTAAAAACAGAGGTAAGGGG

Downstream 100 bases:

>100_bases
GAGGTGGTTTAATGGTTTGGAGGAAGAAGGACGAGTTGATTACTGTTCAGGATATAGCCTTACCATACGAAAGGCTAGCT
TACATACATCTTCAGGATTT

Product: hypothetical protein

Products: NA

Alternate protein names: HMC operon ORF 6 [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MPEYIFNFISFEEFCEVEKRILRLRLSELSHVPFSEEPELQEPNPEWSEKYVTELDGYIAIDEPWKPQNEEEEKELVERF
LSGLRKLLDREANWTFLQPLMLSLEYCARCQTCSEACPIYVSSGRKEIYSPLFRSEMFRKIIRETKGGKRRGRLTVKAIL
RLAELAYRCTLCRRCTLACPLGIDNGLITREIRKLFSQEMGIAPSSLHMQGSVQQLVDGTSVGFTPIVMEDFVREIEFMV
SQVLGRRYYLPVDVKGVDILLIHSVADYIHHPETIAAMAIIFEMAGYEWTLSSKAPALDAVNYGMWYDDIQLARIALSHV
KVASELDVERIVVGESGHAHSTLLTTSERFFVELFPVESYLPLLWKIVRRREIKFDADLNDFPVTLHDPCYITRFTGIIK
PQRDILKDVCPQFREMKPSGVKNYCCGGGGGLAVINTMNFPDFRARIASRLKVKQIVDAFNSDVSEKVQKYVCAPCSNCK
MTINHALKHFGLNRFNMKCVGIPEIVLNCVENVEKKILSIV

Sequences:

>Translated_521_residues
MPEYIFNFISFEEFCEVEKRILRLRLSELSHVPFSEEPELQEPNPEWSEKYVTELDGYIAIDEPWKPQNEEEEKELVERF
LSGLRKLLDREANWTFLQPLMLSLEYCARCQTCSEACPIYVSSGRKEIYSPLFRSEMFRKIIRETKGGKRRGRLTVKAIL
RLAELAYRCTLCRRCTLACPLGIDNGLITREIRKLFSQEMGIAPSSLHMQGSVQQLVDGTSVGFTPIVMEDFVREIEFMV
SQVLGRRYYLPVDVKGVDILLIHSVADYIHHPETIAAMAIIFEMAGYEWTLSSKAPALDAVNYGMWYDDIQLARIALSHV
KVASELDVERIVVGESGHAHSTLLTTSERFFVELFPVESYLPLLWKIVRRREIKFDADLNDFPVTLHDPCYITRFTGIIK
PQRDILKDVCPQFREMKPSGVKNYCCGGGGGLAVINTMNFPDFRARIASRLKVKQIVDAFNSDVSEKVQKYVCAPCSNCK
MTINHALKHFGLNRFNMKCVGIPEIVLNCVENVEKKILSIV
>Mature_520_residues
PEYIFNFISFEEFCEVEKRILRLRLSELSHVPFSEEPELQEPNPEWSEKYVTELDGYIAIDEPWKPQNEEEEKELVERFL
SGLRKLLDREANWTFLQPLMLSLEYCARCQTCSEACPIYVSSGRKEIYSPLFRSEMFRKIIRETKGGKRRGRLTVKAILR
LAELAYRCTLCRRCTLACPLGIDNGLITREIRKLFSQEMGIAPSSLHMQGSVQQLVDGTSVGFTPIVMEDFVREIEFMVS
QVLGRRYYLPVDVKGVDILLIHSVADYIHHPETIAAMAIIFEMAGYEWTLSSKAPALDAVNYGMWYDDIQLARIALSHVK
VASELDVERIVVGESGHAHSTLLTTSERFFVELFPVESYLPLLWKIVRRREIKFDADLNDFPVTLHDPCYITRFTGIIKP
QRDILKDVCPQFREMKPSGVKNYCCGGGGGLAVINTMNFPDFRARIASRLKVKQIVDAFNSDVSEKVQKYVCAPCSNCKM
TINHALKHFGLNRFNMKCVGIPEIVLNCVENVEKKILSIV

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 59713; Mature: 59582

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYIFNFISFEEFCEVEKRILRLRLSELSHVPFSEEPELQEPNPEWSEKYVTELDGYIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
IDEPWKPQNEEEEKELVERFLSGLRKLLDREANWTFLQPLMLSLEYCARCQTCSEACPIY
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
VSSGRKEIYSPLFRSEMFRKIIRETKGGKRRGRLTVKAILRLAELAYRCTLCRRCTLACP
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGIDNGLITREIRKLFSQEMGIAPSSLHMQGSVQQLVDGTSVGFTPIVMEDFVREIEFMV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SQVLGRRYYLPVDVKGVDILLIHSVADYIHHPETIAAMAIIFEMAGYEWTLSSKAPALDA
HHHHCCEEEEEEECCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCHHH
VNYGMWYDDIQLARIALSHVKVASELDVERIVVGESGHAHSTLLTTSERFFVELFPVESY
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHEEECCCEEEEEEECHHHH
LPLLWKIVRRREIKFDADLNDFPVTLHDPCYITRFTGIIKPQRDILKDVCPQFREMKPSG
HHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCC
VKNYCCGGGGGLAVINTMNFPDFRARIASRLKVKQIVDAFNSDVSEKVQKYVCAPCSNCK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
MTINHALKHFGLNRFNMKCVGIPEIVLNCVENVEKKILSIV
HHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEYIFNFISFEEFCEVEKRILRLRLSELSHVPFSEEPELQEPNPEWSEKYVTELDGYIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
IDEPWKPQNEEEEKELVERFLSGLRKLLDREANWTFLQPLMLSLEYCARCQTCSEACPIY
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
VSSGRKEIYSPLFRSEMFRKIIRETKGGKRRGRLTVKAILRLAELAYRCTLCRRCTLACP
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGIDNGLITREIRKLFSQEMGIAPSSLHMQGSVQQLVDGTSVGFTPIVMEDFVREIEFMV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SQVLGRRYYLPVDVKGVDILLIHSVADYIHHPETIAAMAIIFEMAGYEWTLSSKAPALDA
HHHHCCEEEEEEECCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCHHH
VNYGMWYDDIQLARIALSHVKVASELDVERIVVGESGHAHSTLLTTSERFFVELFPVESY
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHEEECCCEEEEEEECHHHH
LPLLWKIVRRREIKFDADLNDFPVTLHDPCYITRFTGIIKPQRDILKDVCPQFREMKPSG
HHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCC
VKNYCCGGGGGLAVINTMNFPDFRARIASRLKVKQIVDAFNSDVSEKVQKYVCAPCSNCK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
MTINHALKHFGLNRFNMKCVGIPEIVLNCVENVEKKILSIV
HHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]