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The map label for this gene is gspE-1

Identifier: 11497950

GI number: 11497950

Start: 308760

End: 310547

Strand: Reverse

Name: gspE-1

Synonym: AF0338

Alternate gene names: 11497950

Gene position: 310547-308760 (Counterclockwise)

Preceding gene: 11497953

Following gene: 11497949

Centisome position: 14.26

GC content: 48.66

Gene sequence:

>1788_bases
GTGCTCAATGGCATCGTCACCGAAAACTGTCTTTGGTACAACAAACCACCACATGATGAGAGGATGATTGAACAATATAA
AATGTTTTTCAATGTTCATTTTGTCATTCTTTCACTTTTACACGAAACATTTTTTAATTTTAAATACCGAAATCTTCTGA
TGCCCACTCTTCGCTTCAGCTTCAAAAAGAAGGAGGATGAAGTTCCAAGAAGAACTGCTGTTTTCGAGATAATAAAAAAA
GGTGCAAAGGAGGAGTTTCTCGGTGAGGAAACCTATAGAACACTCTCAAAGAGGCTTTCGCAGGAATTTGAACCTCCTGA
AGGGTGGATTGAGGTTGAGGGTTATCCCGTTTACGGCAGATTCGTAATCTCAAGCGTTCACATCCTCTATAATGAGGAAG
ATAACGAGTTCATTTATCACGTCAACGAGCCCAAGCTGAGTGAAGAGGAGGAGAAAATTGTCAAGAATGTGATCGGCAGG
CTTGAGTACTATGCCGCAAACCCCAAGGAAATTGGGGACAAAGTTGGAACGCTCAAAAAGAAAATAGATTCCATCCTTGA
GGCTTACAGAGTAAACCTTGAAGGTTCGGATTACTTCAGAGTTCTTTACCACATTTTCAAGCGAACTATCCTTTACGGCA
AGATAACACCCATAATGTACGACCCGATGATCGAGGACATTTCATGCAACGGATACGGCAAGTACATCTACATCTTCCAC
CGCAAGTACACAAACCTCCGCACCAACGTCATCTTCGACGATCCGGATGAGCTTGACAGCTTCGTTGTCAACCTCGCCCA
GAAGTGCGGAAAGCACATCAGCATCGCAGAGCCAATGGTTGACGCGACAATGCCCGACGGCAGCAGAATCCAGATGACTT
TGGGGAGGGAGGTGACCGACCACGGCTCGACCTTCACCATAAGAAAGTTCCGCGAAGAGCCCGTCACTCCCATCGACCTA
ATAGCATGGAAAACCTTCAGCAGCGAGCAGATGGCATATCTATGGCTCTGCATCGAGAACAAGAAGTCACTAATCTTTGC
CGGCGGCACTGCGAGCGGTAAAACCACTTCGATGAACGCCATCTCGCTCTTCATCCCCCGCCGCTCCAAGATAGTGACAA
TTGAAGACACAAGAGAGCTTATGCTCCCCCACGAGAACTGGATTCCTGCTGTAACAAGAGATGCCTTCCACGGAGAGAAG
GGAGCAGTTGACATGTACGATTTGCTCAGAGCGGCATTGAGGCAGAGGCCTGAGTACATAATAGTCGGTGAGGTTAGAGG
GAGGGAGGCTTTAACGCTCTTCCAGGCGATGGCTACAGGCCACACAACCTACTCAACACTGCACGCTGACAGCATAAGCG
GTGCGATACACAGGCTTGAAAACCCGCCAATTGGAGTGCCGAGGCCGATGCTCGAGGCTCTTGACATTATAAGCATCCAG
GCCCAAACTTACGTTGGGGACAGGAGAGTTAGGAGGAACATGGAGATTGCGGAGATTGTCGGTTTGGATGCGCACACAAA
GATGCTGAGAACCTCAACCGTATTCCAGTGGGACAGCGTTAAGGACGAGCACGCGATGGTTGGAACTTCCAAAGCTCTGG
AGGAGATAAGGAGGCAGAGAGGGTGGAGTGTGAGGGAGCTGAACGAGGAGCTTGAGAGGAGGAGGAGAGTGCTGGAGTTT
ATGCTCTCCAACGGAATAAGAATGTTCAAAGACGTCTCTGCGGTCATACATACCTACCAGGTGAATCCCGAGAGGGCGAT
GAAGCGCCTCGGCGTTGAGGAACTCTGA

Upstream 100 bases:

>100_bases
TCTCCGCCTTGAGATTTTTCTTTACCTCCTCAAGGTAGCTCATCTCGCTCAGAACCTTGTCAGTTACAATCAGCACCCTC
TCTGCTCTCTCGCTCTGCAG

Downstream 100 bases:

>100_bases
AATTTATTTTACTGCATTCTTGGCACTGCAATCTAAAATATTGTTAGTTTAGAAAAGAATCGCCGAATTTGCCAAATTTA
CCATTAGAGGGAAGATTGGG

Product: type II secretion system protein (gspE-1)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MLNGIVTENCLWYNKPPHDERMIEQYKMFFNVHFVILSLLHETFFNFKYRNLLMPTLRFSFKKKEDEVPRRTAVFEIIKK
GAKEEFLGEETYRTLSKRLSQEFEPPEGWIEVEGYPVYGRFVISSVHILYNEEDNEFIYHVNEPKLSEEEEKIVKNVIGR
LEYYAANPKEIGDKVGTLKKKIDSILEAYRVNLEGSDYFRVLYHIFKRTILYGKITPIMYDPMIEDISCNGYGKYIYIFH
RKYTNLRTNVIFDDPDELDSFVVNLAQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDL
IAWKTFSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEK
GAVDMYDLLRAALRQRPEYIIVGEVRGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQ
AQTYVGDRRVRRNMEIAEIVGLDAHTKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEF
MLSNGIRMFKDVSAVIHTYQVNPERAMKRLGVEEL

Sequences:

>Translated_595_residues
MLNGIVTENCLWYNKPPHDERMIEQYKMFFNVHFVILSLLHETFFNFKYRNLLMPTLRFSFKKKEDEVPRRTAVFEIIKK
GAKEEFLGEETYRTLSKRLSQEFEPPEGWIEVEGYPVYGRFVISSVHILYNEEDNEFIYHVNEPKLSEEEEKIVKNVIGR
LEYYAANPKEIGDKVGTLKKKIDSILEAYRVNLEGSDYFRVLYHIFKRTILYGKITPIMYDPMIEDISCNGYGKYIYIFH
RKYTNLRTNVIFDDPDELDSFVVNLAQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDL
IAWKTFSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEK
GAVDMYDLLRAALRQRPEYIIVGEVRGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQ
AQTYVGDRRVRRNMEIAEIVGLDAHTKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEF
MLSNGIRMFKDVSAVIHTYQVNPERAMKRLGVEEL
>Mature_595_residues
MLNGIVTENCLWYNKPPHDERMIEQYKMFFNVHFVILSLLHETFFNFKYRNLLMPTLRFSFKKKEDEVPRRTAVFEIIKK
GAKEEFLGEETYRTLSKRLSQEFEPPEGWIEVEGYPVYGRFVISSVHILYNEEDNEFIYHVNEPKLSEEEEKIVKNVIGR
LEYYAANPKEIGDKVGTLKKKIDSILEAYRVNLEGSDYFRVLYHIFKRTILYGKITPIMYDPMIEDISCNGYGKYIYIFH
RKYTNLRTNVIFDDPDELDSFVVNLAQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDL
IAWKTFSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEK
GAVDMYDLLRAALRQRPEYIIVGEVRGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQ
AQTYVGDRRVRRNMEIAEIVGLDAHTKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEF
MLSNGIRMFKDVSAVIHTYQVNPERAMKRLGVEEL

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=121, Percent_Identity=39.6694214876033, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 69132; Mature: 69132

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNGIVTENCLWYNKPPHDERMIEQYKMFFNVHFVILSLLHETFFNFKYRNLLMPTLRFS
CCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FKKKEDEVPRRTAVFEIIKKGAKEEFLGEETYRTLSKRLSQEFEPPEGWIEVEGYPVYGR
HCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCEEEEHH
FVISSVHILYNEEDNEFIYHVNEPKLSEEEEKIVKNVIGRLEYYAANPKEIGDKVGTLKK
HHHEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KIDSILEAYRVNLEGSDYFRVLYHIFKRTILYGKITPIMYDPMIEDISCNGYGKYIYIFH
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEE
RKYTNLRTNVIFDDPDELDSFVVNLAQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTD
EECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEECC
HGSTFTIRKFREEPVTPIDLIAWKTFSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNA
CCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCE
ISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYI
EEEEECCCCEEEEEECCHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCEE
IVGEVRGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQ
EEECCCCCHHHHHHHHHHCCCCHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEE
AQTYVGDRRVRRNMEIAEIVGLDAHTKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQR
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHEEEECCCCCCHHHCCCHHHHHHHHHHC
GWSVRELNEELERRRRVLEFMLSNGIRMFKDVSAVIHTYQVNPERAMKRLGVEEL
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHCCCCCC
>Mature Secondary Structure
MLNGIVTENCLWYNKPPHDERMIEQYKMFFNVHFVILSLLHETFFNFKYRNLLMPTLRFS
CCCCEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FKKKEDEVPRRTAVFEIIKKGAKEEFLGEETYRTLSKRLSQEFEPPEGWIEVEGYPVYGR
HCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCEEEEHH
FVISSVHILYNEEDNEFIYHVNEPKLSEEEEKIVKNVIGRLEYYAANPKEIGDKVGTLKK
HHHEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KIDSILEAYRVNLEGSDYFRVLYHIFKRTILYGKITPIMYDPMIEDISCNGYGKYIYIFH
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEEEEE
RKYTNLRTNVIFDDPDELDSFVVNLAQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTD
EECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEECC
HGSTFTIRKFREEPVTPIDLIAWKTFSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNA
CCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCE
ISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYI
EEEEECCCCEEEEEECCHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCEE
IVGEVRGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQ
EEECCCCCHHHHHHHHHHCCCCHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEE
AQTYVGDRRVRRNMEIAEIVGLDAHTKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQR
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHEEEECCCCCCHHHCCCHHHHHHHHHHC
GWSVRELNEELERRRRVLEFMLSNGIRMFKDVSAVIHTYQVNPERAMKRLGVEEL
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]