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Identifier: 11497864

GI number: 11497864

Start: 225074

End: 226210

Strand: Reverse

Name: Not Available

Synonym: AF0248

Alternate gene names: 11497864

Gene position: 226210-225074 (Counterclockwise)

Preceding gene: 11497867

Following gene: 11497862

Centisome position: 10.38

GC content: 50.66

Gene sequence:

>1137_bases
ATGCCTATCTTCATATCCTACCGCTCTTTAAACCAGAACTTTTTTCAATACTCGATCAATAAAAGCTACATGCTTTTCAC
ACCTATAGAAACTGAGCACCTGAGAATGAAGAACCGCCTCGTCAGATCAGCAACGGCAGAATCGATGGCCACGAAGGAAG
GTTTTGTGACTGAGGAGATGATAGAACTCTACACGAAGCTTGCGGAAGGTGGTGCTGGAACAATAGTGACGGGCTACATG
TTCGTGAGCGAGGATGGCAGAGCCTCCTTCAAGATGACCGGCATAAGCGATGACAGCCACATCGAGGGGTTGTCAAAGCT
GGTTGAAGCTGTCAAGGCTGCTGCAAAGGATGCTGTCTTTGTTGCACAGCTTGCCCACGCTGGAAGGCAGACTTTTCTGG
GCAACGCCATTGCCCCTTCAGCGGTAAAGGACCCTTTCACGGGAGTTGAGCCGAGGGAGATGACAAGAGAGGACATTGAG
AGAGTGATAGAAGCCTTTGTGAAGGCAGCCCTCAGGGCTGAGAAGGCTGGCTTCGATGCGGTGCAGCTTCACGCAGCCCA
CGGTTATCTGTTAAGCGAGTTCATCTCCCCCCATGTCAACAGAAGAAAGGACGAGTACGGGCAGGAGAGGGCAAAGATTT
TGCTCGACATCTTCGATGGCATAAGGCAGAGGAGCAGAATCCCCGTCATGATTAAGATGAACGCCTGCGACTTCGTTCCC
GGAGGGCTTGAAATTGACGAGGCTGTCAGGATTGCAAAGCTACTCGACGATGCTGGCTTTGAGGCCATTGAGGTGAGCGG
GGGAATGTATGAATCCATCTACCACAACCGCTACAACGTTGTCAGTCAGAAGGTTGCTAAGAGCGGGGAGGCTTACTTCA
GGGAATACGCCTTCAGGATAAAGGAGGCTGTTAGCATTCCAGTAATGGCTGTGGGAGGGATAAAAAGCAGGCAAACTGCA
GAGGAACTGCTGAAAAAGGTGGACTTCGTTTCAATGTCCAGACCTCTGATCAGAGATCCCTACCTGCCGAAAAAGATGAT
GAACGGAGAGGTTGATGCCTCAGACTGCAAATCCTGCAACCTCTGCCTCACAGCAATCTTCGAAGGAAAACCCTTAGCAT
GCTACGCTAAGAACTGA

Upstream 100 bases:

>100_bases
TTGAAGTAATTAACTTTTTTCGTTATTTTGGCGGCGACAGTTTTCTTAATTTTAAAAAAATAACCTTTTTAAAAAAACTT
TTACTATTACGTCAACCTAT

Downstream 100 bases:

>100_bases
GAAGCTTGAGGACTGCAGGTAAAATATCCATAATCATCCCCCTTTCTTTCCACTCCTCCTTCAATCCCGGCTTGTGGATT
AGGCCGAAACCAACGGACTC

Product: NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPIFISYRSLNQNFFQYSINKSYMLFTPIETEHLRMKNRLVRSATAESMATKEGFVTEEMIELYTKLAEGGAGTIVTGYM
FVSEDGRASFKMTGISDDSHIEGLSKLVEAVKAAAKDAVFVAQLAHAGRQTFLGNAIAPSAVKDPFTGVEPREMTREDIE
RVIEAFVKAALRAEKAGFDAVQLHAAHGYLLSEFISPHVNRRKDEYGQERAKILLDIFDGIRQRSRIPVMIKMNACDFVP
GGLEIDEAVRIAKLLDDAGFEAIEVSGGMYESIYHNRYNVVSQKVAKSGEAYFREYAFRIKEAVSIPVMAVGGIKSRQTA
EELLKKVDFVSMSRPLIRDPYLPKKMMNGEVDASDCKSCNLCLTAIFEGKPLACYAKN

Sequences:

>Translated_378_residues
MPIFISYRSLNQNFFQYSINKSYMLFTPIETEHLRMKNRLVRSATAESMATKEGFVTEEMIELYTKLAEGGAGTIVTGYM
FVSEDGRASFKMTGISDDSHIEGLSKLVEAVKAAAKDAVFVAQLAHAGRQTFLGNAIAPSAVKDPFTGVEPREMTREDIE
RVIEAFVKAALRAEKAGFDAVQLHAAHGYLLSEFISPHVNRRKDEYGQERAKILLDIFDGIRQRSRIPVMIKMNACDFVP
GGLEIDEAVRIAKLLDDAGFEAIEVSGGMYESIYHNRYNVVSQKVAKSGEAYFREYAFRIKEAVSIPVMAVGGIKSRQTA
EELLKKVDFVSMSRPLIRDPYLPKKMMNGEVDASDCKSCNLCLTAIFEGKPLACYAKN
>Mature_377_residues
PIFISYRSLNQNFFQYSINKSYMLFTPIETEHLRMKNRLVRSATAESMATKEGFVTEEMIELYTKLAEGGAGTIVTGYMF
VSEDGRASFKMTGISDDSHIEGLSKLVEAVKAAAKDAVFVAQLAHAGRQTFLGNAIAPSAVKDPFTGVEPREMTREDIER
VIEAFVKAALRAEKAGFDAVQLHAAHGYLLSEFISPHVNRRKDEYGQERAKILLDIFDGIRQRSRIPVMIKMNACDFVPG
GLEIDEAVRIAKLLDDAGFEAIEVSGGMYESIYHNRYNVVSQKVAKSGEAYFREYAFRIKEAVSIPVMAVGGIKSRQTAE
ELLKKVDFVSMSRPLIRDPYLPKKMMNGEVDASDCKSCNLCLTAIFEGKPLACYAKN

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=360, Percent_Identity=26.9444444444444, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787939, Length=358, Percent_Identity=25.6983240223464, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=349, Percent_Identity=32.378223495702, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17565138, Length=355, Percent_Identity=32.6760563380282, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17564188, Length=386, Percent_Identity=29.5336787564767, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI72001454, Length=348, Percent_Identity=30.7471264367816, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=29.6296296296296, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17559804, Length=371, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=349, Percent_Identity=26.647564469914, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17566916, Length=358, Percent_Identity=24.8603351955307, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=220, Percent_Identity=26.8181818181818, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=217, Percent_Identity=26.7281105990783, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 42097; Mature: 41966

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFISYRSLNQNFFQYSINKSYMLFTPIETEHLRMKNRLVRSATAESMATKEGFVTEEM
CCEEEEEHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IELYTKLAEGGAGTIVTGYMFVSEDGRASFKMTGISDDSHIEGLSKLVEAVKAAAKDAVF
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VAQLAHAGRQTFLGNAIAPSAVKDPFTGVEPREMTREDIERVIEAFVKAALRAEKAGFDA
HHHHHHCCHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VQLHAAHGYLLSEFISPHVNRRKDEYGQERAKILLDIFDGIRQRSRIPVMIKMNACDFVP
EEEEHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GGLEIDEAVRIAKLLDDAGFEAIEVSGGMYESIYHNRYNVVSQKVAKSGEAYFREYAFRI
CCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
KEAVSIPVMAVGGIKSRQTAEELLKKVDFVSMSRPLIRDPYLPKKMMNGEVDASDCKSCN
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHCCCCC
LCLTAIFEGKPLACYAKN
EEEEEEECCCCCEEEECC
>Mature Secondary Structure 
PIFISYRSLNQNFFQYSINKSYMLFTPIETEHLRMKNRLVRSATAESMATKEGFVTEEM
CEEEEEHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IELYTKLAEGGAGTIVTGYMFVSEDGRASFKMTGISDDSHIEGLSKLVEAVKAAAKDAVF
HHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VAQLAHAGRQTFLGNAIAPSAVKDPFTGVEPREMTREDIERVIEAFVKAALRAEKAGFDA
HHHHHHCCHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VQLHAAHGYLLSEFISPHVNRRKDEYGQERAKILLDIFDGIRQRSRIPVMIKMNACDFVP
EEEEHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GGLEIDEAVRIAKLLDDAGFEAIEVSGGMYESIYHNRYNVVSQKVAKSGEAYFREYAFRI
CCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
KEAVSIPVMAVGGIKSRQTAEELLKKVDFVSMSRPLIRDPYLPKKMMNGEVDASDCKSCN
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHCCCCC
LCLTAIFEGKPLACYAKN
EEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]