Click here to switch to the map view.

The map label for this gene is nifS-1

Identifier: 11497803

GI number: 11497803

Start: 164609

End: 165787

Strand: Reverse

Name: nifS-1

Synonym: AF0186

Alternate gene names: 11497803

Gene position: 165787-164609 (Counterclockwise)

Preceding gene: 11497804

Following gene: 11497802

Centisome position: 7.61

GC content: 49.96

Gene sequence:

>1179_bases
ATGGTGTGCTCTATTTCTGAGGTGGTGGAAATGCCCTACTTTGACTACGCCTCCACAAAGCCTGTGGATGAGAGGGTTGT
TGAAGCAATGCTTCCCTACATGACCGAGCACTTCGGAAATCCTTCCTCAGTCCACTCCTACGGCTTTAAAGCAAGAGAGG
TGATTGAGGAGGCAAGGGGGAAGATTTCGGAGCTGATAAATGGTGGTGGCGGGGACATCGTTTTCACCTCAGGTGCCACG
GAGTCCAACAACCTCGCTCTCATTGGATATGCGATGAGAAACGCCAGAAAGGGTAAGCACGTTCTCGTTTCATCAATTGA
GCACATGTCCGTCATAAATCCGGCAAAGTATCTTCAGAAGCAGGGATTTGAGGTTGAATTCATCCCAGTGGACAAATACG
GCACCGTCGACCTTGAATTTATTGAGGAGAAAATCAGGGAAGACACGATTCTTGTTTCTGTTCAGCATGCAAACAACGAA
ATTGGAACGATTCAGCCCATAAAGGAGATTTCAGAAATAATTAACGGTAGAGCAGCTCTGCACGTTGATGCTACAGCCTC
TCTCGGGCAAATTGAGGTGGATGTTGAGAAGATCGGTGCTGATATGCTGACTATTTCAAGCAACGACATTTACGGCCCCA
AAGGAGTGGGGGCGCTTTGGGTAAGGCAGGGAGTGAGGTTGCAGCCGATAATTCTCGGAGGTGGGCAGGAAAAAGGGCTC
AGGAGTGGTAGTGAAAACGTTCCTGCGATAGTTGGATTTGGTAAAGCCGCTGAAATCACCGCAAAGGAGTGGGGAGAGGA
GGCTGGGAGGCTCAGAAGGTTGAGAGATAGGATAATCGATAACGTTCTGAAAATTGAGGAAAGCTACCTAAACGGCCATC
CTGAAAAAAGGCTGCCCAACAATGTAAACGTGAGATTCAGCTACATTGAGGGAGAGTCAATCGTTTTGAGCCTTGACATG
GCGGGAATTCAGGCTTCAACCGGCTCGGCGTGCAGCTCAAAAACCTTGCAGCCAAGCCATGTTCTCATGGCGTGTGGGCT
GAAGCATGAGGAGGCGCACGGCACTCTGCTTTTGACGCTGGGCAGATACAACACTGACGAAGATGTTGACAGGCTGCTTG
AGGTGCTGCCGGGCGTGATTGAGAGGCTGAGGAGCATGTCTCCTCTCTACAGGAGGTGA

Upstream 100 bases:

>100_bases
GACAGAAGCTCCGAACTCCTTGGGATTGATAAGGGAGAGATTGTTGATGGTGTCAAGATTGTAGGTGCTGCAACGCTCAA
CCAGCTCGTTCTTGAGGCGG

Downstream 100 bases:

>100_bases
GGGCTATGTATAGTGATAAGGTTTTCGACCACTTCCAGAATCCGAGGAATGTTGGAAAAATTGAGGATGCTGATGGTGTA
GGAACTGTCGGCAATCCAGT

Product: nifS protein, class-V aminotransferase (nifS-1)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MVCSISEVVEMPYFDYASTKPVDERVVEAMLPYMTEHFGNPSSVHSYGFKAREVIEEARGKISELINGGGGDIVFTSGAT
ESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQKQGFEVEFIPVDKYGTVDLEFIEEKIREDTILVSVQHANNE
IGTIQPIKEISEIINGRAALHVDATASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQGVRLQPIILGGGQEKGL
RSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESYLNGHPEKRLPNNVNVRFSYIEGESIVLSLDM
AGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRSMSPLYRR

Sequences:

>Translated_392_residues
MVCSISEVVEMPYFDYASTKPVDERVVEAMLPYMTEHFGNPSSVHSYGFKAREVIEEARGKISELINGGGGDIVFTSGAT
ESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQKQGFEVEFIPVDKYGTVDLEFIEEKIREDTILVSVQHANNE
IGTIQPIKEISEIINGRAALHVDATASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQGVRLQPIILGGGQEKGL
RSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESYLNGHPEKRLPNNVNVRFSYIEGESIVLSLDM
AGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRSMSPLYRR
>Mature_392_residues
MVCSISEVVEMPYFDYASTKPVDERVVEAMLPYMTEHFGNPSSVHSYGFKAREVIEEARGKISELINGGGGDIVFTSGAT
ESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQKQGFEVEFIPVDKYGTVDLEFIEEKIREDTILVSVQHANNE
IGTIQPIKEISEIINGRAALHVDATASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQGVRLQPIILGGGQEKGL
RSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESYLNGHPEKRLPNNVNVRFSYIEGESIVLSLDM
AGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRSMSPLYRR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=41.2532637075718, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=35.1941747572816, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=45.1948051948052, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1787970, Length=390, Percent_Identity=25.8974358974359, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789175, Length=394, Percent_Identity=24.1116751269036, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=43.4895833333333, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.4159021406728, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=43.717277486911, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=40.9921671018277, Blast_Score=304, Evalue=8e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43061; Mature: 43061

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCSISEVVEMPYFDYASTKPVDERVVEAMLPYMTEHFGNPSSVHSYGFKAREVIEEARG
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHH
KISELINGGGGDIVFTSGATESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQK
HHHHHHCCCCCCEEEECCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCHHHHHHH
QGFEVEFIPVDKYGTVDLEFIEEKIREDTILVSVQHANNEIGTIQPIKEISEIINGRAAL
CCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEE
HVDATASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQGVRLQPIILGGGQEKGL
EEECCCCCCEEEEEHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCC
RSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESYLNGHPEKRLPN
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
NVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTL
CCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEE
GRYNTDEDVDRLLEVLPGVIERLRSMSPLYRR
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MVCSISEVVEMPYFDYASTKPVDERVVEAMLPYMTEHFGNPSSVHSYGFKAREVIEEARG
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHH
KISELINGGGGDIVFTSGATESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQK
HHHHHHCCCCCCEEEECCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCHHHHHHH
QGFEVEFIPVDKYGTVDLEFIEEKIREDTILVSVQHANNEIGTIQPIKEISEIINGRAAL
CCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEE
HVDATASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQGVRLQPIILGGGQEKGL
EEECCCCCCEEEEEHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCC
RSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESYLNGHPEKRLPN
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
NVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTL
CCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEE
GRYNTDEDVDRLLEVLPGVIERLRSMSPLYRR
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]