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The map label for this gene is nifU-1

Identifier: 11497802

GI number: 11497802

Start: 164142

End: 164603

Strand: Reverse

Name: nifU-1

Synonym: AF0185

Alternate gene names: 11497802

Gene position: 164603-164142 (Counterclockwise)

Preceding gene: 11497803

Following gene: 11497801

Centisome position: 7.56

GC content: 45.67

Gene sequence:

>462_bases
ATGTATAGTGATAAGGTTTTCGACCACTTCCAGAATCCGAGGAATGTTGGAAAAATTGAGGATGCTGATGGTGTAGGAAC
TGTCGGCAATCCAGTTTGCGGGGATTTGATGACCATTTACATTAAAGTGAAGGACAACAGAATTGAGGATATCAAGTTCC
AGACCTTTGGCTGCGCTGCTGCCATTGCAACCAGCAGCATGGCCACTGAGATGGCGAAGGGGAAAACTATAGAGGAAGCT
CTGAAAATTACAAGAGATGCTGTTGCCGAAGCCCTTGGCGGTTTGCCGAAGCAGAAAATGCACTGCTCCAACTTGGCCGC
AGATGCGCTGAGGAGGGCAATAGTTGACTACTTTAGAAAAAATGGAAAAATTGATAAGATAAAAGAGTTAGGTCTGGAAA
AGGAACTTGAGAAGATGGAGAAGGGTGAGATGGACGACCACGGAGAGTATTGTGAGGCTTGA

Upstream 100 bases:

>100_bases
GATACAACACTGACGAAGATGTTGACAGGCTGCTTGAGGTGCTGCCGGGCGTGATTGAGAGGCTGAGGAGCATGTCTCCT
CTCTACAGGAGGTGAGGGCT

Downstream 100 bases:

>100_bases
GCCATGAAACCGCACTGCGTGATGATGGTGAAATACGTACTCCCCGCCCTGAGGGCGAAGGTTGCGCTTGAGCTAATAGA
TAGAGGTTACAGGGTAAAGG

Product: nifU protein (nifU-1)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MYSDKVFDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAAAIATSSMATEMAKGKTIEEA
LKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRKNGKIDKIKELGLEKELEKMEKGEMDDHGEYCEA

Sequences:

>Translated_153_residues
MYSDKVFDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAAAIATSSMATEMAKGKTIEEA
LKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRKNGKIDKIKELGLEKELEKMEKGEMDDHGEYCEA
>Mature_153_residues
MYSDKVFDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAAAIATSSMATEMAKGKTIEEA
LKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRKNGKIDKIKELGLEKELEKMEKGEMDDHGEYCEA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=121, Percent_Identity=50.4132231404959, Blast_Score=123, Evalue=7e-29,
Organism=Homo sapiens, GI24307953, Length=119, Percent_Identity=50.4201680672269, Blast_Score=120, Evalue=6e-28,
Organism=Escherichia coli, GI1788878, Length=123, Percent_Identity=52.0325203252033, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17543474, Length=126, Percent_Identity=51.5873015873016, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=47.5409836065574, Blast_Score=117, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=48, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355597, Length=127, Percent_Identity=49.6062992125984, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 16921; Mature: 16921

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSDKVFDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEECCCCCCHHHHHHHHHHH
AIATSSMATEMAKGKTIEEALKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NGKIDKIKELGLEKELEKMEKGEMDDHGEYCEA
CCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MYSDKVFDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAA
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEECCCCCCHHHHHHHHHHH
AIATSSMATEMAKGKTIEEALKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NGKIDKIKELGLEKELEKMEKGEMDDHGEYCEA
CCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]