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The map label for this gene is radB

Identifier: 15679687

GI number: 15679687

Start: 1549429

End: 1550133

Strand: Direct

Name: radB

Synonym: MTH1693

Alternate gene names: 15679687

Gene position: 1549429-1550133 (Clockwise)

Preceding gene: 15679686

Following gene: 15679688

Centisome position: 88.47

GC content: 49.93

Gene sequence:

>705_bases
ATGAAGAAATTAAGGGATATGGGTGAAAACCGGAGGATACCCACGGAGTCCTCCATCGACAGGATACTGGGAGGGGGTGT
GGAAAGAAGAACCATAACACAGTTCTATGGACCCCCCGGCTCAGGTAAGACCAACATAACCATCAAACTCGCGGTTGAAA
CTGCAAGGAGGGGTAAAAACACGGTTTTCATTGATACAGAGGGCGGTCTTTCTGTTGAGAGAATAAGACAGGTCTCAGGT
GATATATTCGACAGGGTTGCAGATAGCATCATAGTATTTGAACCCTCAAGCTTTACAGAGCAGGGTGAGGCACTTCAGAG
GACATTTTCATTCCTCAAAACACATGGGGACTCAACTGACCTGGTGGTCCTTGACTCTGCGGTCGCCCTCTACAGATTGA
AGGAGGGCAATGCATCCAGCTTTAACCTTGACCTTGGAAGGCAGATGTTCCTCCTTCTACAGATGGCCAGAAGGTTTGAC
CTTGCAGCTGTCATAACCAACCAGATATACTCCATTACGGGGGACGATGGAAGGGAATATGTGAGTCCTGTTGGCGGCAC
CCTCCTCCGTTACTGGTCCAAGGTCATGGTCGAGCTTGAGATGGGTGAGAGACCTGGTGAGAGATTCGCTGTTCTCAGGA
GGCACAGGAATCGTCTGGAGGGTTCCCGTGTGGGGTTCAGGATTGTTGCTGATGGCATTCTATGA

Upstream 100 bases:

>100_bases
AGCCATCCACTGTGATTGACCTCACAGTAAACCCTCCAAGGGTTCTGAGGAGGGGTAAGGGTCCTCTTGACCCTGTACTC
CTGAGAGGGGCTGGAGATGT

Downstream 100 bases:

>100_bases
ACCATGACTGGACTGTTTTTAGCCAGCTGTAGGATAATGTGGCCTGATTTTATTACAGAGGCTCCTCTGGTAAACAAAGT
TTATTAAGAGGTTTGTTTAA

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKKLRDMGENRRIPTESSIDRILGGGVERRTITQFYGPPGSGKTNITIKLAVETARRGKNTVFIDTEGGLSVERIRQVSG
DIFDRVADSIIVFEPSSFTEQGEALQRTFSFLKTHGDSTDLVVLDSAVALYRLKEGNASSFNLDLGRQMFLLLQMARRFD
LAAVITNQIYSITGDDGREYVSPVGGTLLRYWSKVMVELEMGERPGERFAVLRRHRNRLEGSRVGFRIVADGIL

Sequences:

>Translated_234_residues
MKKLRDMGENRRIPTESSIDRILGGGVERRTITQFYGPPGSGKTNITIKLAVETARRGKNTVFIDTEGGLSVERIRQVSG
DIFDRVADSIIVFEPSSFTEQGEALQRTFSFLKTHGDSTDLVVLDSAVALYRLKEGNASSFNLDLGRQMFLLLQMARRFD
LAAVITNQIYSITGDDGREYVSPVGGTLLRYWSKVMVELEMGERPGERFAVLRRHRNRLEGSRVGFRIVADGIL
>Mature_234_residues
MKKLRDMGENRRIPTESSIDRILGGGVERRTITQFYGPPGSGKTNITIKLAVETARRGKNTVFIDTEGGLSVERIRQVSG
DIFDRVADSIIVFEPSSFTEQGEALQRTFSFLKTHGDSTDLVVLDSAVALYRLKEGNASSFNLDLGRQMFLLLQMARRFD
LAAVITNQIYSITGDDGREYVSPVGGTLLRYWSKVMVELEMGERPGERFAVLRRHRNRLEGSRVGFRIVADGIL

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily

Homologues:

Organism=Homo sapiens, GI19924133, Length=236, Percent_Identity=29.6610169491525, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI256017143, Length=242, Percent_Identity=29.3388429752066, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI256017141, Length=242, Percent_Identity=29.3388429752066, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI23238219, Length=203, Percent_Identity=27.0935960591133, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321027, Length=214, Percent_Identity=30.3738317757009, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864108, Length=237, Percent_Identity=28.6919831223629, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651285, Length=237, Percent_Identity=28.6919831223629, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADB_METTH (O27728)

Other databases:

- EMBL:   AE000666
- PIR:   D69093
- RefSeq:   NP_276804.1
- ProteinModelPortal:   O27728
- SMR:   O27728
- STRING:   O27728
- GeneID:   1470778
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1693
- NMPDR:   fig|187420.1.peg.1659
- eggNOG:   arNOG05079
- HOGENOM:   HBG413235
- OMA:   ITQIYGP
- PhylomeDB:   O27728
- ProtClustDB:   PRK09361
- BioCyc:   MTHE187420:MTH1693-MONOMER
- HAMAP:   MF_00350
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588
- PANTHER:   PTHR22942:SF9
- PIRSF:   PIRSF003336
- SMART:   SM00382
- TIGRFAMs:   TIGR02237

Pfam domain/function: PF08423 Rad51

EC number: NA

Molecular weight: Translated: 26193; Mature: 26193

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLRDMGENRRIPTESSIDRILGGGVERRTITQFYGPPGSGKTNITIKLAVETARRGKN
CCCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEHHHCCCC
TVFIDTEGGLSVERIRQVSGDIFDRVADSIIVFEPSSFTEQGEALQRTFSFLKTHGDSTD
EEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
LVVLDSAVALYRLKEGNASSFNLDLGRQMFLLLQMARRFDLAAVITNQIYSITGDDGREY
EEEEECCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHH
VSPVGGTLLRYWSKVMVELEMGERPGERFAVLRRHRNRLEGSRVGFRIVADGIL
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKKLRDMGENRRIPTESSIDRILGGGVERRTITQFYGPPGSGKTNITIKLAVETARRGKN
CCCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEHHHCCCC
TVFIDTEGGLSVERIRQVSGDIFDRVADSIIVFEPSSFTEQGEALQRTFSFLKTHGDSTD
EEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCC
LVVLDSAVALYRLKEGNASSFNLDLGRQMFLLLQMARRFDLAAVITNQIYSITGDDGREY
EEEEECCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHH
VSPVGGTLLRYWSKVMVELEMGERPGERFAVLRRHRNRLEGSRVGFRIVADGIL
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463