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The map label for this gene is hisH

Identifier: 15679521

GI number: 15679521

Start: 1385136

End: 1385732

Strand: Direct

Name: hisH

Synonym: MTH1524

Alternate gene names: 15679521

Gene position: 1385136-1385732 (Clockwise)

Preceding gene: 15679520

Following gene: 15679522

Centisome position: 79.09

GC content: 52.09

Gene sequence:

>597_bases
TTGATAGCCATCATAGACTATGGAAGCGGAAACCTCAGGAGCATAGCCAATGCATTCAGGAAGATAGGGGCAGATGCCCT
TGTGACATCAGATCCACAGATCCTTGAAGCCGCCGACGCACTCGTACTCCCGGGTGTCGGTGCATTTGGAAGTGCGATGG
CCAAACTCGAGGGTCTGAGGGAAACCCTGCTGGGGAACATCATGGATGGGAAACCCTTCCTGGGGATATGCCTGGGGCTA
CAGGTGCTCCTTTCAGAGAGCCAGGAATCTCCTGGTGTCCATGGTCTTGACCTTATACCGGGAAGGGTCATAAGAATACC
ACCAGGCAATAAGGTCCCCCATATGGGCTGGAACCAGCTGATAATTGTGGAAGATTCACAGCTCCTTGAGGGTGCTGAGG
ATGAGTACTTCTACTTCGTCCACTCCTACTATGCTGAACCCTCCAACGACGTGGTCGTTGCAAGGACAGATTATGGGGTT
GAAATGACAGCGGCCATCGAATCCGATAACATCCATGCAACCCAGTTCCACCCTGAAAAGAGCGGGGAAGCCGGACTGGA
TGTTCTAAGGAACTTTGTCGAAATAATCAGGGCATGA

Upstream 100 bases:

>100_bases
CTCCATAGTGGCCATGGAGAACACCGTACTCCATGAGGTATCAACACCACACCTAAACGACACCGTGAGGGTCCAGGATT
ACTATACAAGGTGAGATCGC

Downstream 100 bases:

>100_bases
AAGGATCAGTGAGAGGTTGGATAATGGATATAGAGGGATTTGTAAGGCGAAACATGGATTCCATGGATGAGGAGTCACTC
AGGAACGCACTGGCTGACCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MIAIIDYGSGNLRSIANAFRKIGADALVTSDPQILEAADALVLPGVGAFGSAMAKLEGLRETLLGNIMDGKPFLGICLGL
QVLLSESQESPGVHGLDLIPGRVIRIPPGNKVPHMGWNQLIIVEDSQLLEGAEDEYFYFVHSYYAEPSNDVVVARTDYGV
EMTAAIESDNIHATQFHPEKSGEAGLDVLRNFVEIIRA

Sequences:

>Translated_198_residues
MIAIIDYGSGNLRSIANAFRKIGADALVTSDPQILEAADALVLPGVGAFGSAMAKLEGLRETLLGNIMDGKPFLGICLGL
QVLLSESQESPGVHGLDLIPGRVIRIPPGNKVPHMGWNQLIIVEDSQLLEGAEDEYFYFVHSYYAEPSNDVVVARTDYGV
EMTAAIESDNIHATQFHPEKSGEAGLDVLRNFVEIIRA
>Mature_198_residues
MIAIIDYGSGNLRSIANAFRKIGADALVTSDPQILEAADALVLPGVGAFGSAMAKLEGLRETLLGNIMDGKPFLGICLGL
QVLLSESQESPGVHGLDLIPGRVIRIPPGNKVPHMGWNQLIIVEDSQLLEGAEDEYFYFVHSYYAEPSNDVVVARTDYGV
EMTAAIESDNIHATQFHPEKSGEAGLDVLRNFVEIIRA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=42.4242424242424, Blast_Score=150, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=38.0952380952381, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_METTH (O27568)

Other databases:

- EMBL:   AE000666
- PIR:   D69070
- RefSeq:   NP_276638.1
- ProteinModelPortal:   O27568
- SMR:   O27568
- STRING:   O27568
- GeneID:   1471793
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1524
- NMPDR:   fig|187420.1.peg.1493
- eggNOG:   arNOG06706
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   O27568
- ProtClustDB:   PRK13143
- BioCyc:   MTHE187420:MTH1524-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21349; Mature: 21349

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 77-77 ACT_SITE 177-177 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGSGNLRSIANAFRKIGADALVTSDPQILEAADALVLPGVGAFGSAMAKLEGLR
CEEEEECCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCEEEECCCCHHHHHHHHHHHHH
ETLLGNIMDGKPFLGICLGLQVLLSESQESPGVHGLDLIPGRVIRIPPGNKVPHMGWNQL
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCEE
IIVEDSQLLEGAEDEYFYFVHSYYAEPSNDVVVARTDYGVEMTAAIESDNIHATQFHPEK
EEEECHHHHCCCCCCEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCEEEEEECCCC
SGEAGLDVLRNFVEIIRA
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAIIDYGSGNLRSIANAFRKIGADALVTSDPQILEAADALVLPGVGAFGSAMAKLEGLR
CEEEEECCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCEEEECCCCHHHHHHHHHHHHH
ETLLGNIMDGKPFLGICLGLQVLLSESQESPGVHGLDLIPGRVIRIPPGNKVPHMGWNQL
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCEE
IIVEDSQLLEGAEDEYFYFVHSYYAEPSNDVVVARTDYGVEMTAAIESDNIHATQFHPEK
EEEECHHHHCCCCCCEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCEEEEEECCCC
SGEAGLDVLRNFVEIIRA
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463