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Identifier: 15679479

GI number: 15679479

Start: 1338872

End: 1340080

Strand: Direct

Name: Not Available

Synonym: MTH1482

Alternate gene names: 15679479

Gene position: 1338872-1340080 (Clockwise)

Preceding gene: 15679478

Following gene: 15679480

Centisome position: 76.45

GC content: 51.12

Gene sequence:

>1209_bases
TTGAGCTGGTATGATATGGGGCCAGTTTCAACCTGCAAGCTCTTCGGTGCCGTGAGGGCGGTGACAGGCATAAAAAATGC
AGTACCCCTCATCCATGGCCCCAGGGGATGCGCATACCATATAGGCTATCTACTCACAGCCAGGGGCGGGCGGAGGATAG
CTGTGGCATCAACAGAACTATCAGAATCAGACGTGGTCTTCGGAGCATCAGAGAAACTCAGGGACAGGATAATAGAGGTT
GACAGGACACGAAAACCTGATATCATAGTAATTATGAGTTCCTGTGCAACCAGCATAATCGGAGAGGATATAGGGCGGGT
TGCAGATGAGACCTCAGAACTCGTGGACGCGGAGCTCATAACCGTCAGTGCCGGTGGATTTGAATCCAGTCAGGGTGAGG
GATATGAGGAGGTGATGATGGCACTCATCCATAAATTCTGCTGGAGGATGGAGCCATCATCTAAGCCTTCAGTCAACATA
ATAGGTGAATTCAGGGGCGGAGCCGACCTCAGGGAGGTCAGGGAGTCCCTATCATTGATGGGGGTGGATGTGAACTGTGT
CCTGACATCAGGATGCAGCATCAGGGACATGACCATGATCCCGGCGGGCCACCTCAACTGCTCCTTCTGTGATGTCTCGG
GTATCGCCCCCTGCCGCTTCCTTGAGGAGAACTTCGGGACACCCTTCATCCACCACCCCATCCCCATAGGTTTTTCAAAT
TCACTCAGATTTTACAGGGAGGTTCTCGGGTATCTCAACCTCGATTATCCCATAAATGAGGAATTCTCAGCTTTTATCGA
GGAGAGGGATCGAATAAGGGCTGAACTTGAGGGTCTGAGGGTGGCGGTAGTTTCAGGACCAACAAGGGCCGTTGCACTCA
CAGAATTCATAACTGAACTGCGGATGAAGCCAGTTCTTGTATCAATGGATATGATAGGTGATTACACACTGGAAAACCTT
AGAGGGGTGCCTGGATTGAGGTCAGAGATACTTGTCGGGGTCGATGTTTCTGATATTGAGGAGGCCATCATCAGGACTGA
ACCCGAACTGATACTGGGAGGCCTTGCAGAGACCCGCATATCTGAGGTTTCAGGGGCACCCCTCATCGATGTTATGCATG
GATCCAGCCTCACAGCAGGTTTCAGGGGGGCTGTTAACCTTGGTGCTAGAATGTGCGGGGCTGTTTATGGGGATCATGAT
TGTTATTAA

Upstream 100 bases:

>100_bases
AGGCCACAGGAAGGTCCACCAGGGAGGACATAGTCATGGCGAGTGTTGAGGCAGTACTGGACGCCATAAACAAGATTCTG
AGCCTTAAATAGAAAAGTGA

Downstream 100 bases:

>100_bases
ATTTTTCAGTTACCATATCACAAAGTTTATATAATCTTGGGTTTCTATCTTTATTACAGACACTCTTGGAGGTTAAGTGA
TGTCAGAGGAGAATGTAGTA

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE-like protein

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSWYDMGPVSTCKLFGAVRAVTGIKNAVPLIHGPRGCAYHIGYLLTARGGRRIAVASTELSESDVVFGASEKLRDRIIEV
DRTRKPDIIVIMSSCATSIIGEDIGRVADETSELVDAELITVSAGGFESSQGEGYEEVMMALIHKFCWRMEPSSKPSVNI
IGEFRGGADLREVRESLSLMGVDVNCVLTSGCSIRDMTMIPAGHLNCSFCDVSGIAPCRFLEENFGTPFIHHPIPIGFSN
SLRFYREVLGYLNLDYPINEEFSAFIEERDRIRAELEGLRVAVVSGPTRAVALTEFITELRMKPVLVSMDMIGDYTLENL
RGVPGLRSEILVGVDVSDIEEAIIRTEPELILGGLAETRISEVSGAPLIDVMHGSSLTAGFRGAVNLGARMCGAVYGDHD
CY

Sequences:

>Translated_402_residues
MSWYDMGPVSTCKLFGAVRAVTGIKNAVPLIHGPRGCAYHIGYLLTARGGRRIAVASTELSESDVVFGASEKLRDRIIEV
DRTRKPDIIVIMSSCATSIIGEDIGRVADETSELVDAELITVSAGGFESSQGEGYEEVMMALIHKFCWRMEPSSKPSVNI
IGEFRGGADLREVRESLSLMGVDVNCVLTSGCSIRDMTMIPAGHLNCSFCDVSGIAPCRFLEENFGTPFIHHPIPIGFSN
SLRFYREVLGYLNLDYPINEEFSAFIEERDRIRAELEGLRVAVVSGPTRAVALTEFITELRMKPVLVSMDMIGDYTLENL
RGVPGLRSEILVGVDVSDIEEAIIRTEPELILGGLAETRISEVSGAPLIDVMHGSSLTAGFRGAVNLGARMCGAVYGDHD
CY
>Mature_401_residues
SWYDMGPVSTCKLFGAVRAVTGIKNAVPLIHGPRGCAYHIGYLLTARGGRRIAVASTELSESDVVFGASEKLRDRIIEVD
RTRKPDIIVIMSSCATSIIGEDIGRVADETSELVDAELITVSAGGFESSQGEGYEEVMMALIHKFCWRMEPSSKPSVNII
GEFRGGADLREVRESLSLMGVDVNCVLTSGCSIRDMTMIPAGHLNCSFCDVSGIAPCRFLEENFGTPFIHHPIPIGFSNS
LRFYREVLGYLNLDYPINEEFSAFIEERDRIRAELEGLRVAVVSGPTRAVALTEFITELRMKPVLVSMDMIGDYTLENLR
GVPGLRSEILVGVDVSDIEEAIIRTEPELILGGLAETRISEVSGAPLIDVMHGSSLTAGFRGAVNLGARMCGAVYGDHDC
Y

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 43769; Mature: 43638

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWYDMGPVSTCKLFGAVRAVTGIKNAVPLIHGPRGCAYHIGYLLTARGGRRIAVASTEL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHEEEEEEEECCCCCEEEEEECCC
SESDVVFGASEKLRDRIIEVDRTRKPDIIVIMSSCATSIIGEDIGRVADETSELVDAELI
CCCCEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
TVSAGGFESSQGEGYEEVMMALIHKFCWRMEPSSKPSVNIIGEFRGGADLREVRESLSLM
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
GVDVNCVLTSGCSIRDMTMIPAGHLNCSFCDVSGIAPCRFLEENFGTPFIHHPIPIGFSN
CCCEEEEEECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCH
SLRFYREVLGYLNLDYPINEEFSAFIEERDRIRAELEGLRVAVVSGPTRAVALTEFITEL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHH
RMKPVLVSMDMIGDYTLENLRGVPGLRSEILVGVDVSDIEEAIIRTEPELILGGLAETRI
CCCCEEEEHHHHCCHHHHHHCCCCCCCHHEEEECCHHHHHHHHHHCCCHHHCCCHHHHHH
SEVSGAPLIDVMHGSSLTAGFRGAVNLGARMCGAVYGDHDCY
HHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SWYDMGPVSTCKLFGAVRAVTGIKNAVPLIHGPRGCAYHIGYLLTARGGRRIAVASTEL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHEEEEEEEECCCCCEEEEEECCC
SESDVVFGASEKLRDRIIEVDRTRKPDIIVIMSSCATSIIGEDIGRVADETSELVDAELI
CCCCEEECCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
TVSAGGFESSQGEGYEEVMMALIHKFCWRMEPSSKPSVNIIGEFRGGADLREVRESLSLM
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
GVDVNCVLTSGCSIRDMTMIPAGHLNCSFCDVSGIAPCRFLEENFGTPFIHHPIPIGFSN
CCCEEEEEECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCH
SLRFYREVLGYLNLDYPINEEFSAFIEERDRIRAELEGLRVAVVSGPTRAVALTEFITEL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHH
RMKPVLVSMDMIGDYTLENLRGVPGLRSEILVGVDVSDIEEAIIRTEPELILGGLAETRI
CCCCEEEEHHHHCCHHHHHHCCCCCCCHHEEEECCHHHHHHHHHHCCCHHHCCCHHHHHH
SEVSGAPLIDVMHGSSLTAGFRGAVNLGARMCGAVYGDHDCY
HHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408; 7961410 [H]