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Identifier: 15679443

GI number: 15679443

Start: 1306978

End: 1307883

Strand: Direct

Name: Not Available

Synonym: MTH1446

Alternate gene names: 15679443

Gene position: 1306978-1307883 (Clockwise)

Preceding gene: 15679442

Following gene: 15679447

Centisome position: 74.63

GC content: 53.86

Gene sequence:

>906_bases
ATGAAGCACCTTCTATCTGTATGTGACATGGATAACGTGGTGGACCTCCTTGACCTCGCAGATGACTACAAGGAGGGGAA
GATCAGGGAAAAGATCCTCAGGGGTAAAACACTTGCCATGATCTTTGAGAAGTCATCAACAAGGACCAGGGTATCCTTTG
AGGTTGGGGCATTCCAGATGGGGGCTCAGCCCCTCTACCTCTCTGCATCGGACCTTCAGCTTGGAAGGGGCGAACCAATA
GCGGATACAGCAAGGACCCTCAGCAGATACGTTGATGGAATAATGATAAGGGCCATAAGTCACTCCGACGTGGTTGAACT
TGCAGGCGAGGCATCTGTCCCGGTCATCAATGGTCTGACAGACCTGGAACATCCATGCCAGGCCCTGGCCGATATGCAGA
CCATCAGGGAGAAGCTGGGGGGATTCGATGGAAGGCTGGTTTTTGTGGGTGATGGTAACAATGTCTGCCACTCTCTCCTC
CTCATAACAGCCACCCTGGGGATGGACATGGATGTTGCCTGCCCCCCCGGCTATGAACCTGACCCCGGCATCAGGGAGAT
GGCAGGGAAAATAGCAGATGAGACAGGTTCACGGATAAGGGTAATTCATGATCCCTCCGAGGCTGTCAGGGGGGCCGACG
TGGTTTACACCGATGTCTGGGTGAGCATGGGATATGAAGACGAGGCTGAGGACCGTTTAGAGGTCTTCAGACCATACCAG
GTGAATATGGAGCTCATGGAACTTGCAGCTCCCGAAGCCATATTCATGCACTGCCTGCCGGCTGTGAGGGGCCAGGAGAC
AACAGCCGAGGTTATTGACGGTCCCCATTCGGTTGTCTGGGATCAGGCAGAAAACAGGCTCCATGCCCAGAAGGCCATAA
TGCACTGGCTCATGGGGGATATATGA

Upstream 100 bases:

>100_bases
AAGGGAAGGCTCTACCACAGGAGGGATATAGGTACCAGGGAACTCGTTGAGAAGAGGATTAAACATATGGAGGACCTGCG
GTCCTGAGGGAGTTGGGCAG

Downstream 100 bases:

>100_bases
GTTCCACTCAGCAGGTTTATGGGCTTAAAGCTCATTAAAACCTTATCATATTCATTCAGGACACCAGAATCGTGACGCAG
GGTGTATGGCAGGATCATAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKHLLSVCDMDNVVDLLDLADDYKEGKIREKILRGKTLAMIFEKSSTRTRVSFEVGAFQMGAQPLYLSASDLQLGRGEPI
ADTARTLSRYVDGIMIRAISHSDVVELAGEASVPVINGLTDLEHPCQALADMQTIREKLGGFDGRLVFVGDGNNVCHSLL
LITATLGMDMDVACPPGYEPDPGIREMAGKIADETGSRIRVIHDPSEAVRGADVVYTDVWVSMGYEDEAEDRLEVFRPYQ
VNMELMELAAPEAIFMHCLPAVRGQETTAEVIDGPHSVVWDQAENRLHAQKAIMHWLMGDI

Sequences:

>Translated_301_residues
MKHLLSVCDMDNVVDLLDLADDYKEGKIREKILRGKTLAMIFEKSSTRTRVSFEVGAFQMGAQPLYLSASDLQLGRGEPI
ADTARTLSRYVDGIMIRAISHSDVVELAGEASVPVINGLTDLEHPCQALADMQTIREKLGGFDGRLVFVGDGNNVCHSLL
LITATLGMDMDVACPPGYEPDPGIREMAGKIADETGSRIRVIHDPSEAVRGADVVYTDVWVSMGYEDEAEDRLEVFRPYQ
VNMELMELAAPEAIFMHCLPAVRGQETTAEVIDGPHSVVWDQAENRLHAQKAIMHWLMGDI
>Mature_301_residues
MKHLLSVCDMDNVVDLLDLADDYKEGKIREKILRGKTLAMIFEKSSTRTRVSFEVGAFQMGAQPLYLSASDLQLGRGEPI
ADTARTLSRYVDGIMIRAISHSDVVELAGEASVPVINGLTDLEHPCQALADMQTIREKLGGFDGRLVFVGDGNNVCHSLL
LITATLGMDMDVACPPGYEPDPGIREMAGKIADETGSRIRVIHDPSEAVRGADVVYTDVWVSMGYEDEAEDRLEVFRPYQ
VNMELMELAAPEAIFMHCLPAVRGQETTAEVIDGPHSVVWDQAENRLHAQKAIMHWLMGDI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=291, Percent_Identity=42.9553264604811, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=28.2467532467532, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790703, Length=323, Percent_Identity=42.4148606811146, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=41.6413373860182, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI48994908, Length=307, Percent_Identity=24.7557003257329, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=29.126213592233, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=325, Percent_Identity=27.0769230769231, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=280, Percent_Identity=37.1428571428571, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=27.9742765273312, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=26.2135922330097, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METTH (O27495)

Other databases:

- EMBL:   AE000666
- PIR:   E69059
- RefSeq:   NP_276560.1
- ProteinModelPortal:   O27495
- SMR:   O27495
- STRING:   O27495
- GeneID:   1471715
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1446
- NMPDR:   fig|187420.1.peg.1415
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   GATIHIS
- PhylomeDB:   O27495
- ProtClustDB:   PRK00779
- BioCyc:   MTHE187420:MTH1446-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33186; Mature: 33186

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 97-97 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSVCDMDNVVDLLDLADDYKEGKIREKILRGKTLAMIFEKSSTRTRVSFEVGAFQM
CCHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCEEEEEEECHHHC
GAQPLYLSASDLQLGRGEPIADTARTLSRYVDGIMIRAISHSDVVELAGEASVPVINGLT
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCEECCCH
DLEHPCQALADMQTIREKLGGFDGRLVFVGDGNNVCHSLLLITATLGMDMDVACPPGYEP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DPGIREMAGKIADETGSRIRVIHDPSEAVRGADVVYTDVWVSMGYEDEAEDRLEVFRPYQ
CCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEEHHEECCCCCCCHHHHHHHCCHH
VNMELMELAAPEAIFMHCLPAVRGQETTAEVIDGPHSVVWDQAENRLHAQKAIMHWLMGD
CCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
I
C
>Mature Secondary Structure
MKHLLSVCDMDNVVDLLDLADDYKEGKIREKILRGKTLAMIFEKSSTRTRVSFEVGAFQM
CCHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCEEEEEEECHHHC
GAQPLYLSASDLQLGRGEPIADTARTLSRYVDGIMIRAISHSDVVELAGEASVPVINGLT
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCEECCCH
DLEHPCQALADMQTIREKLGGFDGRLVFVGDGNNVCHSLLLITATLGMDMDVACPPGYEP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DPGIREMAGKIADETGSRIRVIHDPSEAVRGADVVYTDVWVSMGYEDEAEDRLEVFRPYQ
CCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEEHHEECCCCCCCHHHHHHHCCHH
VNMELMELAAPEAIFMHCLPAVRGQETTAEVIDGPHSVVWDQAENRLHAQKAIMHWLMGD
CCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463