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Identifier: 15678425

GI number: 15678425

Start: 336049

End: 336495

Strand: Direct

Name: Not Available

Synonym: MTH397

Alternate gene names: 15678425

Gene position: 336049-336495 (Clockwise)

Preceding gene: 15678424

Following gene: 15678426

Centisome position: 19.19

GC content: 53.91

Gene sequence:

>447_bases
ATGTTGGATGCCCTTAAAAGTATTCTGAGGAAAACATCAATCCATGTATGCCTTGTAAATACAGGGGGGTGCAACGGCTG
TGATATAGAGGTCCTCGCACTCCTCTCACCAAGATATGACCTTGAACAGTACGGTATATACGTCCACCAGAACCCCAGGG
AGGCCGATGTCATACTTGTAACCGGTGCAGTTACAGAGCAGTGGAGGGAGAAACTCCAGAGGATCTACCGCAAGGCACCG
GAGCCAAAGATAGTGGTGGCCCTGGGCAACTGTCCAATATCCGGTGACGTATTCAACCAGGAGGGTGGAAGCGTCTATGC
ACCGGTCTCCGACTTCATACCGGTGGATGCAGAGGTGCCCGGCTGCCCCCCCAGACCATCAGAGATACTTGAAGCAATAC
TGGCGGTGGCGCCCGGGGCCATAGCAGAGAGGGGGAAGAAGAGATGA

Upstream 100 bases:

>100_bases
GAGTACTCCGAGGCCCTCAGTGAGGGCAGGAGGAGGCTCCATGAGATACTGAGATCATGATGAACTTCAATCAATAAAGA
GCAAATGCTGAGGTAAAGAA

Downstream 100 bases:

>100_bases
TACTGCCACTTGGACCCATGCACCCGGGATACAAGGAGCCCATAAGGCTCAAGGTGAAGACCCGGGGTGAAAAGGTTCTC
AAAGCCGAAATAGAATACGG

Product: formate hydrogenlyase, subunit 7

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MLDALKSILRKTSIHVCLVNTGGCNGCDIEVLALLSPRYDLEQYGIYVHQNPREADVILVTGAVTEQWREKLQRIYRKAP
EPKIVVALGNCPISGDVFNQEGGSVYAPVSDFIPVDAEVPGCPPRPSEILEAILAVAPGAIAERGKKR

Sequences:

>Translated_148_residues
MLDALKSILRKTSIHVCLVNTGGCNGCDIEVLALLSPRYDLEQYGIYVHQNPREADVILVTGAVTEQWREKLQRIYRKAP
EPKIVVALGNCPISGDVFNQEGGSVYAPVSDFIPVDAEVPGCPPRPSEILEAILAVAPGAIAERGKKR
>Mature_148_residues
MLDALKSILRKTSIHVCLVNTGGCNGCDIEVLALLSPRYDLEQYGIYVHQNPREADVILVTGAVTEQWREKLQRIYRKAP
EPKIVVALGNCPISGDVFNQEGGSVYAPVSDFIPVDAEVPGCPPRPSEILEAILAVAPGAIAERGKKR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=133, Percent_Identity=33.0827067669173, Blast_Score=90, Evalue=8e-19,
Organism=Escherichia coli, GI1789074, Length=144, Percent_Identity=42.3611111111111, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1788834, Length=134, Percent_Identity=39.5522388059701, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1788624, Length=123, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17509685, Length=114, Percent_Identity=38.5964912280702, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859983, Length=133, Percent_Identity=36.0902255639098, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642371, Length=133, Percent_Identity=36.0902255639098, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651058, Length=135, Percent_Identity=34.0740740740741, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16080; Mature: 16080

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDALKSILRKTSIHVCLVNTGGCNGCDIEVLALLSPRYDLEQYGIYVHQNPREADVILV
CHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCHHHCCEEEECCCCCCCEEEE
TGAVTEQWREKLQRIYRKAPEPKIVVALGNCPISGDVFNQEGGSVYAPVSDFIPVDAEVP
ECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHCCCCCCCCC
GCPPRPSEILEAILAVAPGAIAERGKKR
CCCCCHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MLDALKSILRKTSIHVCLVNTGGCNGCDIEVLALLSPRYDLEQYGIYVHQNPREADVILV
CHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEECCCCCHHHCCEEEECCCCCCCEEEE
TGAVTEQWREKLQRIYRKAPEPKIVVALGNCPISGDVFNQEGGSVYAPVSDFIPVDAEVP
ECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHCCCCCCCCC
GCPPRPSEILEAILAVAPGAIAERGKKR
CCCCCHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]