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Identifier: 15678426

GI number: 15678426

Start: 336492

End: 337604

Strand: Direct

Name: Not Available

Synonym: MTH398

Alternate gene names: 15678426

Gene position: 336492-337604 (Clockwise)

Preceding gene: 15678425

Following gene: 15678427

Centisome position: 19.21

GC content: 53.82

Gene sequence:

>1113_bases
ATGATACTGCCACTTGGACCCATGCACCCGGGATACAAGGAGCCCATAAGGCTCAAGGTGAAGACCCGGGGTGAAAAGGT
TCTCAAAGCCGAAATAGAATACGGATACGTTCACAGGGGAATAGAGAGGGTTATGAGGAACAAGACCTGGCAGAAGGCCA
TCTACCTCTCTGAACGCGTCTGCGGGATATGTTCATACATACACACACAGACCTTTGCAGAGGCCTTTGAGGCCATATCA
GAGGTCGAGGCACCCCCCAGGGCACAGTTCCTCCGTGCATTAACAAACGAGCTTGACAGGATACAGAGTCACCTCATCGC
AAATTCAACCTACTTCAAGGCCCTGGACCATGAGACCATGTTCATGTACATGCTGGCCCTCAGGGAGCCTGTAATGGACG
CCATCGAGCTCCTGACAGGTAACCGGGTCAACATGGGCTGGAACGTGGTGGGTGGCGTGAGGATGGACGCCTCAGAGAGC
CACCTGTCAATGATAAGGGAGATCATAGTTGACCTTGAAAGAGAGTTCGACCGTTACGTTGAGATGTTCGAGCACGGCCC
ACTCATAGGCCTCAGGTCAAGGGAGGTCGGTTACATGAGCCAGGAGGAGGCAGAGAAGGCCAGGGCCGTTGGACCTATAG
GGAGGGCCTCGGGTATAAGGTATGACTTCAGGGAGGACCACCCCACCTACAGGGACCACCTGGACTTCAGGACCATCTGG
AGGGATGATGGCGACAACTTTGCACGGGTCATGAACCGTTTCGATGAGATCAGGGTCTCAATTGACCTCATAAAGCAGGT
CATAGATGACATGCCAGGGGGACCCGTCAGGACGAAGGTTGACGTGAAGGCGGGTTACGGTGAGTGGAGAAACGAGGCCC
CCCGGGGAGAGGTGGCCTACATGATTGAAACCAACGGCAACCTCATAAAGAACATATCCATAAGGACCCCGAGCATAATG
AACATTGACGCCTGCGCAAAGTACATGCTGAGGGACGTGGCAACAGTTGCAGACGCCGTTGCAACATATGCAAGCGCTGA
TCCATGCATAGCATGCGCAGAGAGGGTCATGGTTGTTGATGAAGAGAGTGGGGAGAGGGAGATACTCCTCTAA

Upstream 100 bases:

>100_bases
TGGATGCAGAGGTGCCCGGCTGCCCCCCCAGACCATCAGAGATACTTGAAGCAATACTGGCGGTGGCGCCCGGGGCCATA
GCAGAGAGGGGGAAGAAGAG

Downstream 100 bases:

>100_bases
TAAATGCGTGATGTTGATGTCCTATGTCCTCTGTAATATGGTACCTTTACGAGTTTGCCCGTAAGTCCTGGGCAGAGAAA
TTCGCCAATGCACACACAGA

Product: formate hydrogenlyase, subunit 5

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MILPLGPMHPGYKEPIRLKVKTRGEKVLKAEIEYGYVHRGIERVMRNKTWQKAIYLSERVCGICSYIHTQTFAEAFEAIS
EVEAPPRAQFLRALTNELDRIQSHLIANSTYFKALDHETMFMYMLALREPVMDAIELLTGNRVNMGWNVVGGVRMDASES
HLSMIREIIVDLEREFDRYVEMFEHGPLIGLRSREVGYMSQEEAEKARAVGPIGRASGIRYDFREDHPTYRDHLDFRTIW
RDDGDNFARVMNRFDEIRVSIDLIKQVIDDMPGGPVRTKVDVKAGYGEWRNEAPRGEVAYMIETNGNLIKNISIRTPSIM
NIDACAKYMLRDVATVADAVATYASADPCIACAERVMVVDEESGEREILL

Sequences:

>Translated_370_residues
MILPLGPMHPGYKEPIRLKVKTRGEKVLKAEIEYGYVHRGIERVMRNKTWQKAIYLSERVCGICSYIHTQTFAEAFEAIS
EVEAPPRAQFLRALTNELDRIQSHLIANSTYFKALDHETMFMYMLALREPVMDAIELLTGNRVNMGWNVVGGVRMDASES
HLSMIREIIVDLEREFDRYVEMFEHGPLIGLRSREVGYMSQEEAEKARAVGPIGRASGIRYDFREDHPTYRDHLDFRTIW
RDDGDNFARVMNRFDEIRVSIDLIKQVIDDMPGGPVRTKVDVKAGYGEWRNEAPRGEVAYMIETNGNLIKNISIRTPSIM
NIDACAKYMLRDVATVADAVATYASADPCIACAERVMVVDEESGEREILL
>Mature_370_residues
MILPLGPMHPGYKEPIRLKVKTRGEKVLKAEIEYGYVHRGIERVMRNKTWQKAIYLSERVCGICSYIHTQTFAEAFEAIS
EVEAPPRAQFLRALTNELDRIQSHLIANSTYFKALDHETMFMYMLALREPVMDAIELLTGNRVNMGWNVVGGVRMDASES
HLSMIREIIVDLEREFDRYVEMFEHGPLIGLRSREVGYMSQEEAEKARAVGPIGRASGIRYDFREDHPTYRDHLDFRTIW
RDDGDNFARVMNRFDEIRVSIDLIKQVIDDMPGGPVRTKVDVKAGYGEWRNEAPRGEVAYMIETNGNLIKNISIRTPSIM
NIDACAKYMLRDVATVADAVATYASADPCIACAERVMVVDEESGEREILL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=279, Percent_Identity=27.5985663082437, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI260898743, Length=279, Percent_Identity=27.5985663082437, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1789076, Length=366, Percent_Identity=30.327868852459, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788832, Length=366, Percent_Identity=30.327868852459, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI145693162, Length=279, Percent_Identity=26.8817204301075, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17555284, Length=280, Percent_Identity=28.9285714285714, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17568379, Length=279, Percent_Identity=27.9569892473118, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24638644, Length=371, Percent_Identity=25.3369272237197, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI221459469, Length=377, Percent_Identity=25.1989389920424, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 42290; Mature: 42290

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPLGPMHPGYKEPIRLKVKTRGEKVLKAEIEYGYVHRGIERVMRNKTWQKAIYLSERV
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CGICSYIHTQTFAEAFEAISEVEAPPRAQFLRALTNELDRIQSHLIANSTYFKALDHETM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHH
FMYMLALREPVMDAIELLTGNRVNMGWNVVGGVRMDASESHLSMIREIIVDLEREFDRYV
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMFEHGPLIGLRSREVGYMSQEEAEKARAVGPIGRASGIRYDFREDHPTYRDHLDFRTIW
HHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHEEE
RDDGDNFARVMNRFDEIRVSIDLIKQVIDDMPGGPVRTKVDVKAGYGEWRNEAPRGEVAY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHCCCCCCCCEEE
MIETNGNLIKNISIRTPSIMNIDACAKYMLRDVATVADAVATYASADPCIACAERVMVVD
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEE
EESGEREILL
CCCCCCCCCC
>Mature Secondary Structure
MILPLGPMHPGYKEPIRLKVKTRGEKVLKAEIEYGYVHRGIERVMRNKTWQKAIYLSERV
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CGICSYIHTQTFAEAFEAISEVEAPPRAQFLRALTNELDRIQSHLIANSTYFKALDHETM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHH
FMYMLALREPVMDAIELLTGNRVNMGWNVVGGVRMDASESHLSMIREIIVDLEREFDRYV
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMFEHGPLIGLRSREVGYMSQEEAEKARAVGPIGRASGIRYDFREDHPTYRDHLDFRTIW
HHHHCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHEEE
RDDGDNFARVMNRFDEIRVSIDLIKQVIDDMPGGPVRTKVDVKAGYGEWRNEAPRGEVAY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHCCCCCCCCEEE
MIETNGNLIKNISIRTPSIMNIDACAKYMLRDVATVADAVATYASADPCIACAERVMVVD
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEE
EESGEREILL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]