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Identifier: 15678326

GI number: 15678326

Start: 232741

End: 233010

Strand: Direct

Name: Not Available

Synonym: MTH298

Alternate gene names: 15678326

Gene position: 232741-233010 (Clockwise)

Preceding gene: 15678325

Following gene: 15678327

Centisome position: 13.29

GC content: 56.3

Gene sequence:

>270_bases
ATGGAGAAAGAGGAAAACTTCACGCCACTGGTAAACAGGGTATACTCCCTTGCAAGGAGGGACAGGTGCCCCCACTGTGA
GGAGGAACAGCAGGAGATCAAACTCGACAAGCCCGTCTCAATCGTCGAGGGAGACTACAAGCTCACACCCAGTGAGGTGA
AGGAGCGTCTTGAGAGGATAAGCGATGACGACGCCCTCATCCTCGGTGTCAACCCCCAGGTTGCAAGGCCAGAATGGATG
GTCCTGACAGTCCTCCCGGTCCCCCCGTAA

Upstream 100 bases:

>100_bases
ACACCATACACAAGATCCTGCGGTCAACCTGCAGAAAATGTGGGAGGGTCCTCCTCACCGGGACTGAAATCGAGGAATAT
ATCAGAGGATACTTGAAGCC

Downstream 100 bases:

>100_bases
CGATGAGGCCCTCAATCACCCTCAAAAACGGTGAAAGGTCAGAGGACGACCTGACCCATAAACTGGTCGATATACTCAGG
ATAAACCAGCGCCTCAAGGA

Product: DNA-dependent RNA polymerase, subunit A'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 89; Mature: 89

Protein sequence:

>89_residues
MEKEENFTPLVNRVYSLARRDRCPHCEEEQQEIKLDKPVSIVEGDYKLTPSEVKERLERISDDDALILGVNPQVARPEWM
VLTVLPVPP

Sequences:

>Translated_89_residues
MEKEENFTPLVNRVYSLARRDRCPHCEEEQQEIKLDKPVSIVEGDYKLTPSEVKERLERISDDDALILGVNPQVARPEWM
VLTVLPVPP
>Mature_89_residues
MEKEENFTPLVNRVYSLARRDRCPHCEEEQQEIKLDKPVSIVEGDYKLTPSEVKERLERISDDDALILGVNPQVARPEWM
VLTVLPVPP

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000722
- InterPro:   IPR006592
- InterPro:   IPR007080
- InterPro:   IPR007066
- InterPro:   IPR007083
- InterPro:   IPR007081
- InterPro:   IPR012758 [H]

Pfam domain/function: PF04997 RNA_pol_Rpb1_1; PF00623 RNA_pol_Rpb1_2; PF04983 RNA_pol_Rpb1_3; PF05000 RNA_pol_Rpb1_4; PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 10300; Mature: 10300

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEENFTPLVNRVYSLARRDRCPHCEEEQQEIKLDKPVSIVEGDYKLTPSEVKERLERI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEECCCCEECHHHHHHHHHHC
SDDDALILGVNPQVARPEWMVLTVLPVPP
CCCCEEEEECCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MEKEENFTPLVNRVYSLARRDRCPHCEEEQQEIKLDKPVSIVEGDYKLTPSEVKERLERI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEECCCCEECHHHHHHHHHHC
SDDDALILGVNPQVARPEWMVLTVLPVPP
CCCCEEEEECCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]