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Identifier: 15678325

GI number: 15678325

Start: 232373

End: 232774

Strand: Direct

Name: Not Available

Synonym: MTH297

Alternate gene names: 15678325

Gene position: 232373-232774 (Clockwise)

Preceding gene: 15678324

Following gene: 15678326

Centisome position: 13.27

GC content: 54.73

Gene sequence:

>402_bases
TTGAGAGGAACTCTAAAGAAGATTGGGGGGATAGAATTTGGTCTCATGTCCCCAGAGGATATCAGGAAGATATCCGTCGC
CCAGATCGTCACCCCGGACACCTACAACGAGGACGGGTACCCCATAGAGAACGGACTCATGGATCCCAGGCTCGGTGTGA
TAGACCCCCGGTTCCGCTGCTGGACCTGCGGCGCAAGGGGAGGGGAGTGCCCGGGACACTTCGGGAGCATAAACCTTGCA
AGGCCGGTCATACACGTGGGATTCGCAGACACCATACACAAGATCCTGCGGTCAACCTGCAGAAAATGTGGGAGGGTCCT
CCTCACCGGGACTGAAATCGAGGAATATATCAGAGGATACTTGAAGCCATGGAGAAAGAGGAAAACTTCACGCCACTGGT
AA

Upstream 100 bases:

>100_bases
GGACCCACACAGTCCCCGCAGATGGTGCGGTGATGAAGGCGGTGATTGAGGGACTTATAAGGAAGCTCTGACCAAAAAAT
AACTCAGAGGAGAGAATAAC

Downstream 100 bases:

>100_bases
ACAGGGTATACTCCCTTGCAAGGAGGGACAGGTGCCCCCACTGTGAGGAGGAACAGCAGGAGATCAAACTCGACAAGCCC
GTCTCAATCGTCGAGGGAGA

Product: DNA-dependent RNA polymerase, subunit A'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRGTLKKIGGIEFGLMSPEDIRKISVAQIVTPDTYNEDGYPIENGLMDPRLGVIDPRFRCWTCGARGGECPGHFGSINLA
RPVIHVGFADTIHKILRSTCRKCGRVLLTGTEIEEYIRGYLKPWRKRKTSRHW

Sequences:

>Translated_133_residues
MRGTLKKIGGIEFGLMSPEDIRKISVAQIVTPDTYNEDGYPIENGLMDPRLGVIDPRFRCWTCGARGGECPGHFGSINLA
RPVIHVGFADTIHKILRSTCRKCGRVLLTGTEIEEYIRGYLKPWRKRKTSRHW
>Mature_133_residues
MRGTLKKIGGIEFGLMSPEDIRKISVAQIVTPDTYNEDGYPIENGLMDPRLGVIDPRFRCWTCGARGGECPGHFGSINLA
RPVIHVGFADTIHKILRSTCRKCGRVLLTGTEIEEYIRGYLKPWRKRKTSRHW

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI4505939, Length=106, Percent_Identity=46.2264150943396, Blast_Score=100, Evalue=3e-22,
Organism=Homo sapiens, GI39725938, Length=119, Percent_Identity=35.2941176470588, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71987878, Length=107, Percent_Identity=47.6635514018692, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71998295, Length=93, Percent_Identity=37.6344086021505, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145495, Length=120, Percent_Identity=30.8333333333333, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320061, Length=105, Percent_Identity=48.5714285714286, Blast_Score=109, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324690, Length=122, Percent_Identity=34.4262295081967, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324917, Length=110, Percent_Identity=33.6363636363636, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17530899, Length=130, Percent_Identity=41.5384615384615, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281360912, Length=118, Percent_Identity=31.3559322033898, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17647875, Length=108, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000722
- InterPro:   IPR006592
- InterPro:   IPR007080
- InterPro:   IPR007066
- InterPro:   IPR007083
- InterPro:   IPR007081
- InterPro:   IPR012758 [H]

Pfam domain/function: PF04997 RNA_pol_Rpb1_1; PF00623 RNA_pol_Rpb1_2; PF04983 RNA_pol_Rpb1_3; PF05000 RNA_pol_Rpb1_4; PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 15086; Mature: 15086

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTLKKIGGIEFGLMSPEDIRKISVAQIVTPDTYNEDGYPIENGLMDPRLGVIDPRFRC
CCCCHHHHCCCEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
WTCGARGGECPGHFGSINLARPVIHVGFADTIHKILRSTCRKCGRVLLTGTEIEEYIRGY
EECCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHH
LKPWRKRKTSRHW
HHHHHHHCCCCCC
>Mature Secondary Structure
MRGTLKKIGGIEFGLMSPEDIRKISVAQIVTPDTYNEDGYPIENGLMDPRLGVIDPRFRC
CCCCHHHHCCCEECCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
WTCGARGGECPGHFGSINLARPVIHVGFADTIHKILRSTCRKCGRVLLTGTEIEEYIRGY
EECCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHH
LKPWRKRKTSRHW
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]