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Identifier: 15678236

GI number: 15678236

Start: 149510

End: 150181

Strand: Direct

Name: Not Available

Synonym: MTH208

Alternate gene names: 15678236

Gene position: 149510-150181 (Clockwise)

Preceding gene: 15678235

Following gene: 15678237

Centisome position: 8.54

GC content: 50.74

Gene sequence:

>672_bases
GTGTCCCAGTTGTCTAAGGTGGAGGATGAGATACTCTCCCAGGTGAAAAGGTTCCTGAAACACATAAACAGTAACCTCCC
TGAGGGTATGGAACTGGAATTTGAGGGCTTCTACCGCAGGGGCTTTTTCGTAACAAAGAAGCGATACGCCCTCATAGAGG
ATGACACAATAGTTGCAAAGGGTCTTGAACTTGTAAGGCGTGACTGGGCACCAATAGCTAAAAAAACCCAGCGAAAGGTT
CTTATGGCCATCCTCCGCGATGGATCTCCTGAGAAGGCAAGGGAGATAATAAGGGAAGTCGTGGGGCGCATAAGACGGGG
TGACGTGGAACTCGATGACCTGGTGATACACACCCAGATCACAAGGGACCTCTCTGAGTACAAACAGATAGGTCCCCATG
TCATCGCTGCAAAGAGATCCCTGGAGAAGGGAAGGCGTGTTGAGAGGGGCTCCATAGTTAGATACATCATCGTGAAGGGA
AGGGGCCCCATAAGTCAGAGGGCCTTCCCGGTTGAGGACGCAGAAGGCATGGGATACGACCCCGACTATTACATTGAAAA
CCAGGTGATGGCTGCGGTATCACGCATAATGTCATCCCTGGGTTACTCCACAGAGGATATGAATTCGTTATCCTCGGGTG
AAAGGCAGAGCAGCCTCGACGCGTTCTTTTAA

Upstream 100 bases:

>100_bases
TGAGACAAACCTCGGGAACGTCAGGGTCACATCAAGGCCAGGTCAGGACGGTGTCATAAACGGAGTCCTGATCAGGGAAT
AAAAGGATTGAACCCCAGCG

Downstream 100 bases:

>100_bases
TTGGCTGAAGGCTTTCTTTTAACACTTTTCGTTATTTCTAATCAGCTGGAAGCTATCCTTTGTTTTAAGACACCATCTCC
ATGATTTCCGCATCCCACAG

Product: DNA-dependent DNA polymerase family B protein

Products: NA

Alternate protein names: Vent DNA polymerase; IVPS2; Tli pol-1 intein; IVPS1; Tli pol-2 intein [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSQLSKVEDEILSQVKRFLKHINSNLPEGMELEFEGFYRRGFFVTKKRYALIEDDTIVAKGLELVRRDWAPIAKKTQRKV
LMAILRDGSPEKAREIIREVVGRIRRGDVELDDLVIHTQITRDLSEYKQIGPHVIAAKRSLEKGRRVERGSIVRYIIVKG
RGPISQRAFPVEDAEGMGYDPDYYIENQVMAAVSRIMSSLGYSTEDMNSLSSGERQSSLDAFF

Sequences:

>Translated_223_residues
MSQLSKVEDEILSQVKRFLKHINSNLPEGMELEFEGFYRRGFFVTKKRYALIEDDTIVAKGLELVRRDWAPIAKKTQRKV
LMAILRDGSPEKAREIIREVVGRIRRGDVELDDLVIHTQITRDLSEYKQIGPHVIAAKRSLEKGRRVERGSIVRYIIVKG
RGPISQRAFPVEDAEGMGYDPDYYIENQVMAAVSRIMSSLGYSTEDMNSLSSGERQSSLDAFF
>Mature_222_residues
SQLSKVEDEILSQVKRFLKHINSNLPEGMELEFEGFYRRGFFVTKKRYALIEDDTIVAKGLELVRRDWAPIAKKTQRKVL
MAILRDGSPEKAREIIREVVGRIRRGDVELDDLVIHTQITRDLSEYKQIGPHVIAAKRSLEKGRRVERGSIVRYIIVKGR
GPISQRAFPVEDAEGMGYDPDYYIENQVMAAVSRIMSSLGYSTEDMNSLSSGERQSSLDAFF

Specific function: Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 DOD-type homing endonuclease domains [H]

Homologues:

Organism=Homo sapiens, GI106507301, Length=221, Percent_Identity=27.6018099547511, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI156616275, Length=192, Percent_Identity=28.6458333333333, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786246, Length=237, Percent_Identity=28.6919831223629, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32565317, Length=190, Percent_Identity=30, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320101, Length=192, Percent_Identity=27.0833333333333, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324227, Length=161, Percent_Identity=26.7080745341615, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 25529; Mature: 25397

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLSKVEDEILSQVKRFLKHINSNLPEGMELEFEGFYRRGFFVTKKRYALIEDDTIVAK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCEEEECCCEEEECCCHHHHH
GLELVRRDWAPIAKKTQRKVLMAILRDGSPEKAREIIREVVGRIRRGDVELDDLVIHTQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TRDLSEYKQIGPHVIAAKRSLEKGRRVERGSIVRYIIVKGRGPISQRAFPVEDAEGMGYD
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
PDYYIENQVMAAVSRIMSSLGYSTEDMNSLSSGERQSSLDAFF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SQLSKVEDEILSQVKRFLKHINSNLPEGMELEFEGFYRRGFFVTKKRYALIEDDTIVAK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCEEEECCCEEEECCCHHHHH
GLELVRRDWAPIAKKTQRKVLMAILRDGSPEKAREIIREVVGRIRRGDVELDDLVIHTQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TRDLSEYKQIGPHVIAAKRSLEKGRRVERGSIVRYIIVKGRGPISQRAFPVEDAEGMGYD
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
PDYYIENQVMAAVSRIMSSLGYSTEDMNSLSSGERQSSLDAFF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1608969; 1475179 [H]