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Identifier: 15678222

GI number: 15678222

Start: 137637

End: 139136

Strand: Direct

Name: Not Available

Synonym: MTH194

Alternate gene names: 15678222

Gene position: 137637-139136 (Clockwise)

Preceding gene: 15678221

Following gene: 15678223

Centisome position: 7.86

GC content: 54.4

Gene sequence:

>1500_bases
ATGCCTTTTAAGGTTGAAAGAAAGGAAGAGGTATGTAAGAGGAACTTTGATCGTCCCGGCTGCTGCTGGTACATGTGCGA
TAACAGGGACGAGTCACTCTGTGCAAACTGTTACTCATGCTACAACAACTGTCCACATGACGTATACGAGATAATAAACG
GGGAACCCGTACCAGTAAGGCATGAAAACTGTGTAGGGTGCCGCATATGTGAAGAAATGTGTCCTAACAATGCGATAGAG
GTTAACGCGGTACCTGAAGATCGCAGGAACGTGTGGTCATTTGCTGACCTCCTTGAAATAGAGAGGAAGTCAAGGGAAGG
CTCATACAAGGTCCGTGGCTGCGGTGCCGTCAGGAGGATACCAACCTTTGATGACCTTGTCATCATCCCGGCCCAGGTAT
CCAGGCCACCCATAGACAAGTACAGGGAGCCCTGCAACACCAGGGTCGTGATAGGTGACAGGTACGCCGAGAACCCCCTT
GAACTTGACACACCCATCATGATAGCCGCCATGTCCTTCGGCGCCCTCAGCAAGGAGGCCAAGATAGCCCTCGCAATGGG
TGCCAGCCTCGCAGGAACAGCCACCAACACAGGTGAGGGTGGAATGCTCCCTGAGGAGCGCAGATACGCCTCCAAGCTCA
TTGCACAGTACGCATCAGGCCGCTTCGGTGTATCAGCAGAGTACCTCAACAACTCAGAGGCCATCGAGATAAAGATAGGT
CAGGGCGCCAAGTCAGGTATGGGCGGCCACCTCCTTGCAGAGAAGGTCACAGCCGAGGTCTCAAGGATAAGGATGATACC
GGAGGGGACCGATGCACTGAGCCCTGCCAGGCACATGGACATAGTGGGTCCTGAGGATCTGAGCATGAAGATATCACAGC
TGCGTGAGATAACAGACTGGAAGGTCCCCATAATGGTCAAGTTCACCTCAGGCAGGGTTGCAGATGATGTTAAGATAGCT
GCAAAGGCGGGTGCAGACGCCGTTGTTGTGGATGGTATGCAGGGCGGTACAGGGGCCGGTCCCGATGTTGTAACCGAGCA
CTCAGGTATACCCACAATCGCGGCCATAGTGGAGGCAGATGAGGCACTCAAGGAGGTCAACCTCCGTGATGAGGTGAGTC
TTATCGCTGCAGGGGGTATAAGGAGCGGGGCTGATGTTGCAAAGGCCATAGCCCTCGGTGCAGATGCAGTCTACATAGGT
ACAGCAGCCCTGGTGTCAATCGGCTGCCGTGTCTGCCAGATGTGTTACACAGGCACCTGCAGGAAGGGTATCGCAACCCA
GGACCCCCGCCTCAGGAAGCGCCTTGACTATGTGGAGGCAGGTAAGAACGTTGCAAGGTACATAGAGGCAATGACAGAGG
AGGTCTGCATGCTCACACAGCAGGCAGGTAACACCGATGTGAGCAAACTTGAAAAGGACGACCTCAGGGCCCTTACAGTT
GAAGCCTCCGCACTCACAGGGGTTAAACTGGCAGGTATGGAGTCACCGGCCAGGTTCTAA

Upstream 100 bases:

>100_bases
TGTACTGGCAGAGGAGCCCCAGGACATTGTGAAGGCTGCAGGAACCAAATACTCGGCATGTCCATTACTGAAGGTTTTAA
AGGAATAAGAGGGGTTAAAA

Downstream 100 bases:

>100_bases
TCCCCTAAAACCTTTTTTGATCCTTTTTACTTCAGATAAACTGGATTCAGACAATTTTTCATTTTTCTGTTTTTAAATTT
TTTCATTTCATTCGCTCTGA

Product: glutamate synthase (NADPH), alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPFKVERKEEVCKRNFDRPGCCWYMCDNRDESLCANCYSCYNNCPHDVYEIINGEPVPVRHENCVGCRICEEMCPNNAIE
VNAVPEDRRNVWSFADLLEIERKSREGSYKVRGCGAVRRIPTFDDLVIIPAQVSRPPIDKYREPCNTRVVIGDRYAENPL
ELDTPIMIAAMSFGALSKEAKIALAMGASLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAEYLNNSEAIEIKIG
QGAKSGMGGHLLAEKVTAEVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDWKVPIMVKFTSGRVADDVKIA
AKAGADAVVVDGMQGGTGAGPDVVTEHSGIPTIAAIVEADEALKEVNLRDEVSLIAAGGIRSGADVAKAIALGADAVYIG
TAALVSIGCRVCQMCYTGTCRKGIATQDPRLRKRLDYVEAGKNVARYIEAMTEEVCMLTQQAGNTDVSKLEKDDLRALTV
EASALTGVKLAGMESPARF

Sequences:

>Translated_499_residues
MPFKVERKEEVCKRNFDRPGCCWYMCDNRDESLCANCYSCYNNCPHDVYEIINGEPVPVRHENCVGCRICEEMCPNNAIE
VNAVPEDRRNVWSFADLLEIERKSREGSYKVRGCGAVRRIPTFDDLVIIPAQVSRPPIDKYREPCNTRVVIGDRYAENPL
ELDTPIMIAAMSFGALSKEAKIALAMGASLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAEYLNNSEAIEIKIG
QGAKSGMGGHLLAEKVTAEVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDWKVPIMVKFTSGRVADDVKIA
AKAGADAVVVDGMQGGTGAGPDVVTEHSGIPTIAAIVEADEALKEVNLRDEVSLIAAGGIRSGADVAKAIALGADAVYIG
TAALVSIGCRVCQMCYTGTCRKGIATQDPRLRKRLDYVEAGKNVARYIEAMTEEVCMLTQQAGNTDVSKLEKDDLRALTV
EASALTGVKLAGMESPARF
>Mature_498_residues
PFKVERKEEVCKRNFDRPGCCWYMCDNRDESLCANCYSCYNNCPHDVYEIINGEPVPVRHENCVGCRICEEMCPNNAIEV
NAVPEDRRNVWSFADLLEIERKSREGSYKVRGCGAVRRIPTFDDLVIIPAQVSRPPIDKYREPCNTRVVIGDRYAENPLE
LDTPIMIAAMSFGALSKEAKIALAMGASLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAEYLNNSEAIEIKIGQ
GAKSGMGGHLLAEKVTAEVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDWKVPIMVKFTSGRVADDVKIAA
KAGADAVVVDGMQGGTGAGPDVVTEHSGIPTIAAIVEADEALKEVNLRDEVSLIAAGGIRSGADVAKAIALGADAVYIGT
AALVSIGCRVCQMCYTGTCRKGIATQDPRLRKRLDYVEAGKNVARYIEAMTEEVCMLTQQAGNTDVSKLEKDDLRALTVE
ASALTGVKLAGMESPARF

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=317, Percent_Identity=37.5394321766561, Blast_Score=174, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=323, Percent_Identity=38.0804953560372, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320030, Length=277, Percent_Identity=37.1841155234657, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI28574881, Length=296, Percent_Identity=37.1621621621622, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24665539, Length=296, Percent_Identity=37.1621621621622, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54014; Mature: 53883

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKVERKEEVCKRNFDRPGCCWYMCDNRDESLCANCYSCYNNCPHDVYEIINGEPVPVR
CCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
HENCVGCRICEEMCPNNAIEVNAVPEDRRNVWSFADLLEIERKSREGSYKVRGCGAVRRI
CCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PTFDDLVIIPAQVSRPPIDKYREPCNTRVVIGDRYAENPLELDTPIMIAAMSFGALSKEA
CCCCCEEEEECCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHCCCCCCC
KIALAMGASLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAEYLNNSEAIEIKIG
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEC
QGAKSGMGGHLLAEKVTAEVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDW
CCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCC
KVPIMVKFTSGRVADDVKIAAKAGADAVVVDGMQGGTGAGPDVVTEHSGIPTIAAIVEAD
CCEEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHH
EALKEVNLRDEVSLIAAGGIRSGADVAKAIALGADAVYIGTAALVSIGCRVCQMCYTGTC
HHHHHCCCCHHEEEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
RKGIATQDPRLRKRLDYVEAGKNVARYIEAMTEEVCMLTQQAGNTDVSKLEKDDLRALTV
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEE
EASALTGVKLAGMESPARF
EHHHHCCEEEECCCCCCCC
>Mature Secondary Structure 
PFKVERKEEVCKRNFDRPGCCWYMCDNRDESLCANCYSCYNNCPHDVYEIINGEPVPVR
CCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
HENCVGCRICEEMCPNNAIEVNAVPEDRRNVWSFADLLEIERKSREGSYKVRGCGAVRRI
CCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PTFDDLVIIPAQVSRPPIDKYREPCNTRVVIGDRYAENPLELDTPIMIAAMSFGALSKEA
CCCCCEEEEECCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHCCCCCCC
KIALAMGASLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAEYLNNSEAIEIKIG
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEC
QGAKSGMGGHLLAEKVTAEVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDW
CCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCC
KVPIMVKFTSGRVADDVKIAAKAGADAVVVDGMQGGTGAGPDVVTEHSGIPTIAAIVEAD
CCEEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHH
EALKEVNLRDEVSLIAAGGIRSGADVAKAIALGADAVYIGTAALVSIGCRVCQMCYTGTC
HHHHHCCCCHHEEEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
RKGIATQDPRLRKRLDYVEAGKNVARYIEAMTEEVCMLTQQAGNTDVSKLEKDDLRALTV
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEE
EASALTGVKLAGMESPARF
EHHHHCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]