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Identifier: 15678199

GI number: 15678199

Start: 114471

End: 116243

Strand: Direct

Name: Not Available

Synonym: MTH171

Alternate gene names: 15678199

Gene position: 114471-116243 (Clockwise)

Preceding gene: 15678190

Following gene: 15678202

Centisome position: 6.54

GC content: 54.26

Gene sequence:

>1773_bases
ATGTGTGGCATTGTGGCTTGTATACTTAAGGATGGGAGTGCAGCCCCGGTTCTCCTGGAATGCGTCAGGAGACTGGAGTA
CAGGGGCTATGACTCCGTTGGTATTGCAACATCAGACCCCATGATAAGGATAAAGAAGGACAGCGGCAAAATCGACGAGG
TGGACGCGGAACTTGACCTTGCAGATCTACCAGGCACTATGGGGATAGCCCATGTTAGGTGGGCAACCCATGGTCTCCCC
ACAGCTGAGAATGCCCACCCACACACCGACTGCAGTGGCGAGATAGCAGTTGTACACAACGGGATAATAGAGAACTACCT
TGAGGTTAAGGAGGAACTTGAATCTGAGGGCCACATATTCAGGTCCGAGACAGACACCGAGGTCATACCACACCTCATAG
AGAAGTACATGGATGAGGGAATGGACCTTGAAGCCGCAACAGCCACAGCACTCAGGAAGCTCAGGGGGGCATATGCGATT
GCAGCGGTATCCTCCAGGGAGCCGGGAAGGATCGTGGGGGCGAGAAAGGAGAGCCCCCTCATAGTCGGTGTCGGTGAAGG
TGAATACTTCCTGGCCTCAGATGTGCCAGCCATCCTCAACCACACCAGCAGGGTGATATACCTCGATGACGGTGAGATGG
TCATACTTGATGGGGATCTGAGGGTCACCGACATGGAGGGCAACACTGTTGAGAAGGAGGTCCATTCAATCGACTGGTCC
GCTGACATGGCAGAGAAGGGCGGCTACGACCACTTCATGCTTAAGGAGATACATGAGGAACCCTCAGCGGTGAGGGACAC
CCTCACAGAGTGGGATGAGGTGCTTGGAGTGGTTGAGGAGATAGGCGAGGTTGAAAGGATCTGCTTCGTTGCCTGCGGAA
CATCCTACCACGCATCACTTGTTGGAAAGTACCTCTTTGAGAGCCTCCTGGGCATACCAACCGATGTGATCCTTGCAAGC
GAGTTCAGATACTCAGCAGGGGCCCTGAATGACAGGACACTGGCGATATTCATAAGCCAGTCAGGTGAAACAGCGGATAC
CCTGAACGCCCTCCGCGCAGCCAATTCAAGGGCGAAGACCCTGGCAATAGTCAATGTACTTGGGAGCTCGGCAACAAGGG
AGGCCCAGCATGTTCTATACACAAGGGCAGGACCCGAGATAGGTGTGGCTGCAACCAAGACCTATGTGAGTCAGCTAACA
GTTATATACATGCTCGTGGCTGCAATGGGCGCACCCGAGCTCATGGATAGACTTGAAAGGGTTCCTGAGTTCATGGAGAG
GGCCCTTGAGGATGAGGATGGCATCAAGGAACTTGCAGCCACCTGCAGTGACGTGAGTGACTTCTTCTTCATAGGCCGAG
GATTTGCTTATCCAACGGCCCTTGAGGGTGCCCTGAAGCTCAAGGAGATAACCTACATACACGGTGAGGGCTACGCAGCA
GGGGAACTCAAGCACGGACCCCTGGCCCTGATAGATAATGGGGTGCCGGTCGTTGCGATTTCACCGCCAGGTCCGTGCCA
TGATAAGACCCTGAGCAACGTTGAGGAGGTCAGGGCAAGGGGTGCAAGGGTCATAGGGGTTGGCAGTATTTCAGACGAAT
CCCTCAGGAAGGAGGCTGATGACTTCATAGGCCTTGACCCTGAGGTGGATGATGTCATATCACCCCTGGTCTACATCGTT
CCCCTCCAGCTCCTCTCCTACCATGTGAGTGTGGAACGTGGACTGGACCCTGACAAGCCAAAGAACCTTGCAAAGTGTGT
GACCGTTGAGTGA

Upstream 100 bases:

>100_bases
CTCTCCAATACTTATATAGGTGTAAATTCTATTATGAGTTATATTGCGATTCAGTTATATAATCTTGCGAAATCAGATTC
ATGCTTCTTAGGGTGAAATA

Downstream 100 bases:

>100_bases
AGGTGATCATGGAAAGCAAATACCGGGGCTCTGACGCTGCAATCCCTGAATCTTCAATCTTCCTGAGGGGAGGGTACCTT
AACTGACCATCTCATTGTAA

Product: glucosamine--fructose-6-phosphate aminotransferase

Products: NA

Alternate protein names: D-fructose-6-phosphate amidotransferase; GFAT; Glucosamine-6-phosphate synthase; Hexosephosphate aminotransferase; L-glutamine-D-fructose-6-phosphate amidotransferase

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MCGIVACILKDGSAAPVLLECVRRLEYRGYDSVGIATSDPMIRIKKDSGKIDEVDAELDLADLPGTMGIAHVRWATHGLP
TAENAHPHTDCSGEIAVVHNGIIENYLEVKEELESEGHIFRSETDTEVIPHLIEKYMDEGMDLEAATATALRKLRGAYAI
AAVSSREPGRIVGARKESPLIVGVGEGEYFLASDVPAILNHTSRVIYLDDGEMVILDGDLRVTDMEGNTVEKEVHSIDWS
ADMAEKGGYDHFMLKEIHEEPSAVRDTLTEWDEVLGVVEEIGEVERICFVACGTSYHASLVGKYLFESLLGIPTDVILAS
EFRYSAGALNDRTLAIFISQSGETADTLNALRAANSRAKTLAIVNVLGSSATREAQHVLYTRAGPEIGVAATKTYVSQLT
VIYMLVAAMGAPELMDRLERVPEFMERALEDEDGIKELAATCSDVSDFFFIGRGFAYPTALEGALKLKEITYIHGEGYAA
GELKHGPLALIDNGVPVVAISPPGPCHDKTLSNVEEVRARGARVIGVGSISDESLRKEADDFIGLDPEVDDVISPLVYIV
PLQLLSYHVSVERGLDPDKPKNLAKCVTVE

Sequences:

>Translated_590_residues
MCGIVACILKDGSAAPVLLECVRRLEYRGYDSVGIATSDPMIRIKKDSGKIDEVDAELDLADLPGTMGIAHVRWATHGLP
TAENAHPHTDCSGEIAVVHNGIIENYLEVKEELESEGHIFRSETDTEVIPHLIEKYMDEGMDLEAATATALRKLRGAYAI
AAVSSREPGRIVGARKESPLIVGVGEGEYFLASDVPAILNHTSRVIYLDDGEMVILDGDLRVTDMEGNTVEKEVHSIDWS
ADMAEKGGYDHFMLKEIHEEPSAVRDTLTEWDEVLGVVEEIGEVERICFVACGTSYHASLVGKYLFESLLGIPTDVILAS
EFRYSAGALNDRTLAIFISQSGETADTLNALRAANSRAKTLAIVNVLGSSATREAQHVLYTRAGPEIGVAATKTYVSQLT
VIYMLVAAMGAPELMDRLERVPEFMERALEDEDGIKELAATCSDVSDFFFIGRGFAYPTALEGALKLKEITYIHGEGYAA
GELKHGPLALIDNGVPVVAISPPGPCHDKTLSNVEEVRARGARVIGVGSISDESLRKEADDFIGLDPEVDDVISPLVYIV
PLQLLSYHVSVERGLDPDKPKNLAKCVTVE
>Mature_590_residues
MCGIVACILKDGSAAPVLLECVRRLEYRGYDSVGIATSDPMIRIKKDSGKIDEVDAELDLADLPGTMGIAHVRWATHGLP
TAENAHPHTDCSGEIAVVHNGIIENYLEVKEELESEGHIFRSETDTEVIPHLIEKYMDEGMDLEAATATALRKLRGAYAI
AAVSSREPGRIVGARKESPLIVGVGEGEYFLASDVPAILNHTSRVIYLDDGEMVILDGDLRVTDMEGNTVEKEVHSIDWS
ADMAEKGGYDHFMLKEIHEEPSAVRDTLTEWDEVLGVVEEIGEVERICFVACGTSYHASLVGKYLFESLLGIPTDVILAS
EFRYSAGALNDRTLAIFISQSGETADTLNALRAANSRAKTLAIVNVLGSSATREAQHVLYTRAGPEIGVAATKTYVSQLT
VIYMLVAAMGAPELMDRLERVPEFMERALEDEDGIKELAATCSDVSDFFFIGRGFAYPTALEGALKLKEITYIHGEGYAA
GELKHGPLALIDNGVPVVAISPPGPCHDKTLSNVEEVRARGARVIGVGSISDESLRKEADDFIGLDPEVDDVISPLVYIV
PLQLLSYHVSVERGLDPDKPKNLAKCVTVE

Specific function: Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source

COG id: COG0449

COG function: function code M; Glucosamine 6-phosphate synthetase, contains amidotransferase and phosphosugar isomerase domains

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains

Homologues:

Organism=Homo sapiens, GI4826742, Length=684, Percent_Identity=37.4269005847953, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI205277386, Length=683, Percent_Identity=36.0175695461201, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1790167, Length=614, Percent_Identity=46.0912052117264, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87082251, Length=314, Percent_Identity=23.5668789808917, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788651, Length=225, Percent_Identity=30.2222222222222, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539970, Length=435, Percent_Identity=36.3218390804598, Blast_Score=253, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17532899, Length=435, Percent_Identity=36.551724137931, Blast_Score=251, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17532897, Length=435, Percent_Identity=36.551724137931, Blast_Score=251, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322745, Length=431, Percent_Identity=36.8909512761021, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6323731, Length=430, Percent_Identity=30.4651162790698, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6323730, Length=205, Percent_Identity=39.0243902439024, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323958, Length=242, Percent_Identity=28.5123966942149, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357745, Length=684, Percent_Identity=36.9883040935673, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMS_METTH (O26273)

Other databases:

- EMBL:   AE000666
- PIR:   G69095
- RefSeq:   NP_275314.1
- ProteinModelPortal:   O26273
- STRING:   O26273
- MEROPS:   C44.971
- GeneID:   1470132
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH171
- NMPDR:   fig|187420.1.peg.169
- eggNOG:   arNOG04073
- HOGENOM:   HBG645312
- OMA:   EVEREYR
- PhylomeDB:   O26273
- ProtClustDB:   PRK00331
- BioCyc:   MTHE187420:MTH171-MONOMER
- BRENDA:   2.6.1.16
- GO:   GO:0005737
- HAMAP:   MF_00164
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR005855
- InterPro:   IPR001347
- TIGRFAMs:   TIGR01135

Pfam domain/function: PF00310 GATase_2; PF01380 SIS

EC number: =2.6.1.16

Molecular weight: Translated: 63995; Mature: 63995

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS51278 GATASE_TYPE_2; PS51464 SIS; PS00443 GATASE_TYPE_II

Important sites: ACT_SITE 2-2 ACT_SITE 585-585

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVACILKDGSAAPVLLECVRRLEYRGYDSVGIATSDPMIRIKKDSGKIDEVDAELDL
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCH
ADLPGTMGIAHVRWATHGLPTAENAHPHTDCSGEIAVVHNGIIENYLEVKEELESEGHIF
HHCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEE
RSETDTEVIPHLIEKYMDEGMDLEAATATALRKLRGAYAIAAVSSREPGRIVGARKESPL
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEECCCCCCCE
IVGVGEGEYFLASDVPAILNHTSRVIYLDDGEMVILDGDLRVTDMEGNTVEKEVHSIDWS
EEEECCCCEEEECCCHHHHCCCCEEEEEECCCEEEEECCEEEEECCCCHHHHHHHHCCCC
ADMAEKGGYDHFMLKEIHEEPSAVRDTLTEWDEVLGVVEEIGEVERICFVACGTSYHASL
CCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
VGKYLFESLLGIPTDVILASEFRYSAGALNDRTLAIFISQSGETADTLNALRAANSRAKT
HHHHHHHHHHCCCHHHEEEHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHH
LAIVNVLGSSATREAQHVLYTRAGPEIGVAATKTYVSQLTVIYMLVAAMGAPELMDRLER
HHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VPEFMERALEDEDGIKELAATCSDVSDFFFIGRGFAYPTALEGALKLKEITYIHGEGYAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEEECCCEEC
GELKHGPLALIDNGVPVVAISPPGPCHDKTLSNVEEVRARGARVIGVGSISDESLRKEAD
CCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DFIGLDPEVDDVISPLVYIVPLQLLSYHVSVERGLDPDKPKNLAKCVTVE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECC
>Mature Secondary Structure
MCGIVACILKDGSAAPVLLECVRRLEYRGYDSVGIATSDPMIRIKKDSGKIDEVDAELDL
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCH
ADLPGTMGIAHVRWATHGLPTAENAHPHTDCSGEIAVVHNGIIENYLEVKEELESEGHIF
HHCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEE
RSETDTEVIPHLIEKYMDEGMDLEAATATALRKLRGAYAIAAVSSREPGRIVGARKESPL
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEECCCCCCCE
IVGVGEGEYFLASDVPAILNHTSRVIYLDDGEMVILDGDLRVTDMEGNTVEKEVHSIDWS
EEEECCCCEEEECCCHHHHCCCCEEEEEECCCEEEEECCEEEEECCCCHHHHHHHHCCCC
ADMAEKGGYDHFMLKEIHEEPSAVRDTLTEWDEVLGVVEEIGEVERICFVACGTSYHASL
CCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
VGKYLFESLLGIPTDVILASEFRYSAGALNDRTLAIFISQSGETADTLNALRAANSRAKT
HHHHHHHHHHCCCHHHEEEHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHH
LAIVNVLGSSATREAQHVLYTRAGPEIGVAATKTYVSQLTVIYMLVAAMGAPELMDRLER
HHHHHHHCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VPEFMERALEDEDGIKELAATCSDVSDFFFIGRGFAYPTALEGALKLKEITYIHGEGYAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEEECCCEEC
GELKHGPLALIDNGVPVVAISPPGPCHDKTLSNVEEVRARGARVIGVGSISDESLRKEAD
CCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DFIGLDPEVDDVISPLVYIVPLQLLSYHVSVERGLDPDKPKNLAKCVTVE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463