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Identifier: 15645965

GI number: 15645965

Start: 1412218

End: 1413297

Strand: Reverse

Name: Not Available

Synonym: HP1352

Alternate gene names: 15645965

Gene position: 1413297-1412218 (Counterclockwise)

Preceding gene: 15646203

Following gene: 15645964

Centisome position: 84.74

GC content: 33.24

Gene sequence:

>1080_bases
ATGGATTTTTTAAAAGAAAACTTAAACACTATCATAGAGGGGGATTGTTTAGAAAAATTGAAAGACTTTCCTAACAGAAG
CGTTGATTTTATCTTTGCTGACCCCCCATATTTTATGCAAACAGAGGGGGAATTGAAGCGTTTTGAAGGCACAAAATTTC
AAGGCGTTGAGGATTATTGGGATAAATTTGGCTCTTTTAAGGAATACGATGCCTTTTGTTTGGGTTGGTTGAAAGAATGC
CAAAGGATTTTAAAAGATAATGGCAGTATTTGTGTGATAGGGAGTTTTCAAAATATTTTTAGAATTGGTTTTCATTTGCA
AAATTTAGGGTTTTGGATACTCAATGATATTATTTGGCACAAGAGTAATCCGGTGCCTAATTTTGCTGGCAAGAGATTAT
GCAACGCCCATGAGACGCTTATTTGGTGTGCTAAACACAAAAACAGCAAAGTTGCCTTTAATTATAAAACAATGAAGTAC
CTCAATAACGACAAACAAGAAAAATCGGTTTGGCAAATCCCTATTTGCATGGGTAACGAAAGACTAAAAGATGCGCAAGG
TAAAAAAGTGCATTCCACGCAAAAACCAGAAGCGCTTTTAAAAAAAATCATTTTAAGCGCGACTAAACCTAAAGATATTA
TTTTAGATCCCTTTTTTGGCACAGGCACAACAGGGGCTGTGGCTAAATCCATGAACAGGTATTTTATTGGTATTGAAAAA
GATTCTTTTTATATTAAAGAAGCGGCAAAACGCCTGAATAACACTAGGGATAAAAGCGATTTTATCACTAATTTAGATTT
AGAAACTAAACCCCCAAAAATACCTATGAGTCTTTTAATTTCTAAACAATTATTAAAAATCGGGGATTTTTTATACTCAC
CTAACAAAGAAAAAATTTGTCAAGTTTTAGAAAACGGACAAGTGAGGGATAATGAAAACTATGAAACTTCTATTCATAAG
ATGAGCGCTAAATATTTGAATAAAACCAACCATAATGGCTGGAAATTTTTTTATGCGTATTACCAAAATCAATTTTTATT
GCTAGATGAATTGCGTTATATCTGCCAAAAGGACTCTTAA

Upstream 100 bases:

>100_bases
TGCATTTGATGAGAACAAAGCTAAAGATTAAACATTAAGATAACCTTAAAACGCTTGTGTTAAAATGGCTAGAGTAGCAG
ATATAAAAGGTTAGTTAATC

Downstream 100 bases:

>100_bases
TGGACTATCAAACCTTTAACGAGATTTTTAATCGTTTTGTCTTTGGAACATCTAAAGCGAAATTACTTGAAAATATTGCC
GAAAATCCTGAACGCTATTT

Product: adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.HinfI; Adenine-specific methyltransferase HinfI [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDYWDKFGSFKEYDAFCLGWLKEC
QRILKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVAFNYKTMKY
LNNDKQEKSVWQIPICMGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLDLETKPPKIPMSLLISKQLLKIGDFLYSPNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKDS

Sequences:

>Translated_359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDYWDKFGSFKEYDAFCLGWLKEC
QRILKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVAFNYKTMKY
LNNDKQEKSVWQIPICMGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLDLETKPPKIPMSLLISKQLLKIGDFLYSPNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKDS
>Mature_359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDYWDKFGSFKEYDAFCLGWLKEC
QRILKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVAFNYKTMKY
LNNDKQEKSVWQIPICMGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLDLETKPPKIPMSLLISKQLLKIGDFLYSPNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKDS

Specific function: This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=268, Percent_Identity=35.8208955223881, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41888; Mature: 41888

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDYW
CCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCHHHHCCCCCCCCHHHHH
DKFGSFKEYDAFCLGWLKECQRILKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWH
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
KSNPVPNFAGKRLCNAHETLIWCAKHKNSKVAFNYKTMKYLNNDKQEKSVWQIPICMGNE
CCCCCCCHHHHHHCCCHHHEEEEEECCCCEEEEEHHHHHHHCCCCCCCCEEEEEEEECCC
RLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEC
DSFYIKEAAKRLNNTRDKSDFITNLDLETKPPKIPMSLLISKQLLKIGDFLYSPNKEKIC
CCHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QVLENGQVRDNENYETSIHKMSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKDS
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDYW
CCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCHHHHCCCCCCCCHHHHH
DKFGSFKEYDAFCLGWLKECQRILKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWH
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
KSNPVPNFAGKRLCNAHETLIWCAKHKNSKVAFNYKTMKYLNNDKQEKSVWQIPICMGNE
CCCCCCCHHHHHHCCCHHHEEEEEECCCCEEEEEHHHHHHHCCCCCCCCEEEEEEEECCC
RLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEC
DSFYIKEAAKRLNNTRDKSDFITNLDLETKPPKIPMSLLISKQLLKIGDFLYSPNKEKIC
CCHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QVLENGQVRDNENYETSIHKMSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKDS
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3063606 [H]