Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 15645804

GI number: 15645804

Start: 1260646

End: 1261974

Strand: Reverse

Name: hisS

Synonym: HP1190

Alternate gene names: 15645804

Gene position: 1261974-1260646 (Counterclockwise)

Preceding gene: 15645806

Following gene: 15645803

Centisome position: 75.66

GC content: 38.45

Gene sequence:

>1329_bases
ATGATTACCCCTAAAGTGTTGAGCGGGTTTAAAGACCGCTTGCCTAAAGATGCGATACAAAAAGCCCAGTTGCTTGCGAA
AGTTTCAGTCGTGTTTCAAAGTTTTGGTTTTGTGCCGATTGAAACCCCTCATTTGGAATACGCTCAAACGTTATTGCCTG
ATGCGAGCAGTGATATTCAAAAAGAAATTTATCGTTTTAAAGACCATGGGGATAGAGATGTGGCTTTAAGGTTTGATTTG
ACTGTGCCATTAGCCCGCTTTGTCTCTTTGCACCACCAAACGCTAGGCATGCCCTTTAAACGCTACGCTATAGGCAATGT
CTTTAGGGGCGAAAGGGCGCAAAAAGGGCGTTATAGGGAATTTACGCAATGCGATTTTGATTTTATAGGGAGCGAGAGTT
TGGTGTGCGATGCTGAGATCATTCAAGTGATTGTCGCTTCTTTAAAAGCCCTAGATTTAGAAGATTTTTGCGTCTCTATC
AACCACAGAAAAATTTTGAACGGGATATGCGAATATTTTGGGATCTCTCAAGTGAATGAAGCGTTGCGCATTGTGGATAA
ATTGGAAAAAATTGGCTTGAATGGGGTTGAAGAAGAATTAAAAAAAGAGTGCGGTTTAAATTCAAACACCATTAAAGAGC
TTTTAGAATTAATTCAAATCAAACAAAACGATTTAAGCCATGCGGAATTTTTTGAAAAAATTGCTTATTTGAAAGACTAT
AATGAAAATCTAAAAAAAGGCATACAGGATTTAGAAAGGCTATACCAGTTGCTAGGGGATTTGCAAATTTCTCAAAACCT
GTATAAAATTGATTTTTCTATCGCTAGGGGATTAGGGTATTATACAGGGATTGTGTATGAAACCACGCTTAATGAAATGA
AGTCTTTAGGGAGCGTGTGTTCAGGGGGGCGTTATGATCATTTGACTAAAAATTTTTCTAAAGAGAATTTACAAGGGGTA
GGGGCTTCTATTGGGATTGATCGATTGATTGTGGCTTTGAGTGAAATGCAATTATTAGACGAGCGCTCCACCCAAGCCAA
AGTTTTAATCGCTTGCATGCATGAAGAGTATTTTTCTTACGCCAACCGCTTAGCGGAGTCTTTAAGGCAAAGCGGGATTT
TTAGTGAAGTCTATCCAGAAGCTCAAAAAATCAAAAAACCCTTTTCTTATGCCAACCATAAAGGGCATGAGTTTGTGGCT
GTCATTGGCGAAGAAGAATTTAAAAGCGAAACTTTAAGCTTGAAAAACATGCATTCAGGCATGCAGTTGAATTGCTTGAG
TTTTTTAAAAGCCCTTGAAATCATTGGAGAAAACGATGAAGACTTATAA

Upstream 100 bases:

>100_bases
CAATAAAATACGCATGCGTTTGGGTGCATTTACGCTCATCAATAGCCCTTTTTTAAAGATTTTAAGCTAAAATTTTAGCA
TACAAATACAAGGAAATGGA

Downstream 100 bases:

>100_bases
TGTCGCTATTGTTGGGGCCAGTGGGGCGGTAGGCCAAGAGCTGATTAAGGGTTTAGAAAATTCTTTTTTCCCAATTAAAA
AATTTGTCCCGCTCGCTAGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MITPKVLSGFKDRLPKDAIQKAQLLAKVSVVFQSFGFVPIETPHLEYAQTLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCVSI
NHRKILNGICEYFGISQVNEALRIVDKLEKIGLNGVEEELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVCSGGRYDHLTKNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Sequences:

>Translated_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLAKVSVVFQSFGFVPIETPHLEYAQTLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCVSI
NHRKILNGICEYFGISQVNEALRIVDKLEKIGLNGVEEELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVCSGGRYDHLTKNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL
>Mature_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLAKVSVVFQSFGFVPIETPHLEYAQTLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCVSI
NHRKILNGICEYFGISQVNEALRIVDKLEKIGLNGVEEELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVCSGGRYDHLTKNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=436, Percent_Identity=32.1100917431193, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI15029520, Length=436, Percent_Identity=30.7339449541284, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI65287717, Length=416, Percent_Identity=25.7211538461538, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=25.4716981132075, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993693, Length=440, Percent_Identity=32.5, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71993686, Length=440, Percent_Identity=32.5, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=29.9781181619256, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320489, Length=312, Percent_Identity=22.7564102564103, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24643061, Length=461, Percent_Identity=29.7180043383948, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24643059, Length=477, Percent_Identity=28.3018867924528, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50173; Mature: 50173

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLAKVSVVFQSFGFVPIETPHLEYAQTLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHH
KEIYRFKDHGDRDVALRFDLTVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCVSINHRKILNGICEYFGISQVNE
HHHCCCHHCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCHHHHHH
ALRIVDKLEKIGLNGVEEELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDY
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTKNFSKENLQGVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLAKVSVVFQSFGFVPIETPHLEYAQTLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHH
KEIYRFKDHGDRDVALRFDLTVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCVSINHRKILNGICEYFGISQVNE
HHHCCCHHCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCHHHHHH
ALRIVDKLEKIGLNGVEEELKKECGLNSNTIKELLELIQIKQNDLSHAEFFEKIAYLKDY
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNEMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTKNFSKENLQGVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA