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Identifier: 15645559

GI number: 15645559

Start: 1003675

End: 1004907

Strand: Reverse

Name: Not Available

Synonym: HP0943

Alternate gene names: 15645559

Gene position: 1004907-1003675 (Counterclockwise)

Preceding gene: 15645560

Following gene: 15645558

Centisome position: 60.25

GC content: 41.12

Gene sequence:

>1233_bases
ATGAAAAAAGAGGTCGTGGTCATAGGCGGTGGGATTGTAGGGCTTTCTTGTGCGTATTCTATGCACAAGTTAGGGCATAA
GGTCTGCGTGATAGAAAAAAACGATGGCGCAAACGGCACTTCTTTTGGGAATGCTGGGCTTATTTCTGCGTTTAAAAAAG
CCCCACTCTCATGCCCTGGTGTGGTGTTAGACACCCTGAAGCTCATGCTCAAAAACCAAGCCCCTTTAAAATTCCATTTC
GGGCTTAATTTAAAGCTCTATCAATGGATTTTAAAATTTGTAAAAAGCGCGAACGCCAAATCCACGCACCGCACCATGGC
GTTGTTTGAACGCTACGGGTGGCTGAGTATTGATATGTATCATCAAATGCTAAAAGACGGCATGGACTTTTGGTATAAAG
AAGATGGGCTTTTAATGATCTACACTCTAGAAGAAAGTTTTGAAAAAAAGCTTAAAACTTGCGATAACAGCGGCGCTTAT
AAAATCCTTAGCGCTAAAGAGACCAAAGAATACATGCCCGTTGTTAATGACAATATCTGCGGGAGCGTGCTTTTAACCGA
AAACGCGCATGTGGATCCGGGCGAAGTGATGCACTCTTTGCAAGAATATTTACAAAATGTTGGCGTGGAGTTCCTTTATA
ATGAAGAAGTGATCGATTTTGAGTTTAAAAATAACCTCATTGAGGGCGTTATCACGCACAAGGAAAAAATCCAAGCAGAA
ACAATCATTCTAGCCACTGGGGCTAACCCCACTCTCATTAAAAAAACCAAGAACGATTTTTTAATGATGGGGGCTAAAGG
ATATAGCATCACCTTTAAAATGCCTGAAGAATTAAAACCCAAAACCTCTTCTTTATTTGCGGATATTTTCATGGCGATGA
CCCCACGAAGAGACACTGTAAGGATCACTTCTAAATTAGAATTAAACACCAACAACGCTCTCATTGATAAAGAGCAAATC
GCTAACATGAAAAAGAATTTAGCCGCTTTCACGCAGCCTTTTGAAATGAAAGACGCCATAGAGTGGTGCGGTTTCAGACC
CTTAACCCCTAATGATATTCCTTATTTGGGCTATGACAAACGCTATAAAAACTTAATCCATGCGACAGGGCTAGGGTGGC
TTGGCATCACTTTTGGCCCAGCCATTGGTAAAATCATCGCCAATTTGAGCCAAGACGGAGCGAATGAAAAAAATGCCGAT
ATTATGCTTTTTTCTGCATTTTTTAGGGATTAA

Upstream 100 bases:

>100_bases
TCCGGCCAGAGCGACCTTTCAAGTGGCTAAACTGCCTAAAGACGCTTTAGTAGAAATTGAAGCGATAGCCATTAAGTAAT
TTATTAAAGGGACTATCAGC

Downstream 100 bases:

>100_bases
GGAATTTCTTTTTTAAACCCTAGTTTATTAAGGAGTTTTTATGGAAACGATTGATTCGGTGGTGCGTTTGTTATCTAATT
TGGTGTGGGGGATTCCCATG

Product: D-amino acid dehydrogenase (dadA)

Products: 2-oxo acid; NH3; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKNDGANGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFVKSANAKSTHRTMALFERYGWLSIDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILSAKETKEYMPVVNDNICGSVLLTENAHVDPGEVMHSLQEYLQNVGVEFLYNEEVIDFEFKNNLIEGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD

Sequences:

>Translated_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKNDGANGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFVKSANAKSTHRTMALFERYGWLSIDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILSAKETKEYMPVVNDNICGSVLLTENAHVDPGEVMHSLQEYLQNVGVEFLYNEEVIDFEFKNNLIEGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD
>Mature_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKNDGANGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFVKSANAKSTHRTMALFERYGWLSIDMYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAY
KILSAKETKEYMPVVNDNICGSVLLTENAHVDPGEVMHSLQEYLQNVGVEFLYNEEVIDFEFKNNLIEGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=400, Percent_Identity=26, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1

Molecular weight: Translated: 46113; Mature: 46113

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKNDGANGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFVKSANAKSTHRTMALFERYGWLSIDMY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAYKILSAKETKEYMPVVNDNIC
HHHHHCCCHHEECCCCEEEEEECCHHHHHHHHCCCCCCCEEEEECHHHHHHCCCCCCCCC
GSVLLTENAHVDPGEVMHSLQEYLQNVGVEFLYNEEVIDFEFKNNLIEGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCEE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCEEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQIANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKNDGANGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFVKSANAKSTHRTMALFERYGWLSIDMY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDNSGAYKILSAKETKEYMPVVNDNIC
HHHHHCCCHHEECCCCEEEEEECCHHHHHHHHCCCCCCCEEEEECHHHHHHCCCCCCCCC
GSVLLTENAHVDPGEVMHSLQEYLQNVGVEFLYNEEVIDFEFKNNLIEGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCEE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCEEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQIANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-amino acid; H2O; acceptor

Specific reaction: a D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]