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Identifier: 15645516

GI number: 15645516

Start: 950740

End: 951852

Strand: Reverse

Name: Not Available

Synonym: HP0898

Alternate gene names: 15645516

Gene position: 951852-950740 (Counterclockwise)

Preceding gene: 15645517

Following gene: 15645514

Centisome position: 57.07

GC content: 44.47

Gene sequence:

>1113_bases
ATGAGCGTTGATCACCTCATTTCGCCCTTTAGAGACAAGCGAACCCTTTTAGCGCTCTCTAATGCAATCAAAAAACTCGC
TTTCAAACTTGAAAAAAAATTAGTCATCATGGAAGTGTGCGGAGGGCATACGCATTCTATCATGAAATACGGGCTTTTAG
ATTTGATGCCTAACAATTTAGAGTTTGTGCATGGGCCGGGCTGCCCGGTATGCGTGATGCCAAGAGCGCGCCTTGATGAA
GCTTATGAACTCGCTACTATCAAAGATAGCATTGTTTTGAGTTTAGGGGATATGATGAGAGTCCCCGGGAGCTATGGGAG
TTTGATACAAGCCAGAGAAAAGGGGTTGGATGCACGCTTTTTGTATTCGCCCATGCAAGCTTTAGAGATCGCTAAAGAAA
ACCCTACTAAAAAAGTCATTTATATTGCGATCGGTTTTGAAACCACAACGCCCATGAGCGCTAGCGTTTTATGGAGCGCC
AAAAAAGAAAAAATTAATAACCTTTTTTTCCACATTAACCACATTCTAGTGCCTCCCAGCGTGAGCGCGATTTTAAAAGA
TCCAGCATGCCAGATTAACGCCCTTTTAGCCCCTAGCCATGTGAGCGTGATCAGCGGCGCTCAAATCTATGCTCCTTTAG
TGGATCGCTTTAAAATCCCCATTATCGTGAGCGGTTTTGAGCCGGTGGATATATTAGAAAGCGTGCTGATGCTTATCAAA
CAAGCCCTAAACAAAGAAGCCAAGCTAGAAATCCAATACAAAAGAGCGGTGAGTTTTGAGGGGAATGTGAAAGCGCAAGA
GTTAGTCAATGCTTGCATGGAAGTTAGGGAAAATTTTGAATGGAGAGGTTTGGGGAATATCAAACGATCCGCTCTCAAAC
TCAAAGAAGCGTTCGCTTCTTATGACGCTGAAGAAGTCTTTAAAGAATATTTAAGCCACAAAACCTCTAAAGAAAACAAA
GCATGCAAGTGCGGGGAGATTTTAAAAGGCATCGCTAAGCCCCTAGACTGCTCGTTATTCGCTACAACTTGCACCCCACA
AAACCCGATCGGCAGTTGCATGGTCAGCTCTGAGGGGGCGTGCGCGGCGTATTATCGTTACAAGCGCGTTTGA

Upstream 100 bases:

>100_bases
TCGGCTATGTGATGAGCAAGATTGATGAAAAAGAAGCCCTAGAATCCATTGAGCTTTATCAAGAAATGATCGCCAGAATG
AACGAAACGCAATAATAACA

Downstream 100 bases:

>100_bases
GTTTTGAAAATTGCTCTAAGCCCCTAGACTGCTCACTATTTGCCAAAACTTGCACCCCGCAAAACCCGATCGGCAGTTGC
ATGGTCAGCTCTCAAGGGGG

Product: hydrogenase expression/formation protein (hypD)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSVDHLISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNLEFVHGPGCPVCVMPRARLDE
AYELATIKDSIVLSLGDMMRVPGSYGSLIQAREKGLDARFLYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSA
KKEKINNLFFHINHILVPPSVSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIK
QALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFASYDAEEVFKEYLSHKTSKENK
ACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGACAAYYRYKRV

Sequences:

>Translated_370_residues
MSVDHLISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNLEFVHGPGCPVCVMPRARLDE
AYELATIKDSIVLSLGDMMRVPGSYGSLIQAREKGLDARFLYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSA
KKEKINNLFFHINHILVPPSVSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIK
QALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFASYDAEEVFKEYLSHKTSKENK
ACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGACAAYYRYKRV
>Mature_369_residues
SVDHLISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNLEFVHGPGCPVCVMPRARLDEA
YELATIKDSIVLSLGDMMRVPGSYGSLIQAREKGLDARFLYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSAK
KEKINNLFFHINHILVPPSVSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIKQ
ALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFASYDAEEVFKEYLSHKTSKENKA
CKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGACAAYYRYKRV

Specific function: Required For The Formation Of All Three Hydrogenase Isoenzymes. [C]

COG id: COG0409

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypD family [H]

Homologues:

Organism=Escherichia coli, GI1789084, Length=367, Percent_Identity=43.5967302452316, Blast_Score=345, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002780 [H]

Pfam domain/function: PF01924 HypD [H]

EC number: NA

Molecular weight: Translated: 41063; Mature: 40932

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDHLISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNL
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHCCCCC
EFVHGPGCPVCVMPRARLDEAYELATIKDSIVLSLGDMMRVPGSYGSLIQAREKGLDARF
CEEECCCCCEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
LYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSAKKEKINNLFFHINHILVPPS
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCC
VSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIK
HHHHHCCCCHHEEEEECCCHHHHHCCCHHHHHHHHCEECCEEEECCCHHHHHHHHHHHHH
QALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFAS
HHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
YDAEEVFKEYLSHKTSKENKACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGA
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCC
CAAYYRYKRV
EEEHHHECCC
>Mature Secondary Structure 
SVDHLISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNL
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHCCCCC
EFVHGPGCPVCVMPRARLDEAYELATIKDSIVLSLGDMMRVPGSYGSLIQAREKGLDARF
CEEECCCCCEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
LYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSAKKEKINNLFFHINHILVPPS
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCC
VSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIK
HHHHHCCCCHHEEEEECCCHHHHHCCCHHHHHHHHCEECCEEEECCCHHHHHHHHHHHHH
QALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFAS
HHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
YDAEEVFKEYLSHKTSKENKACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGA
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEECCCCC
CAAYYRYKRV
EEEHHHECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 8510650 [H]