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The map label for this gene is truA

Identifier: 15644989

GI number: 15644989

Start: 371188

End: 371916

Strand: Reverse

Name: truA

Synonym: HP0361

Alternate gene names: 15644989

Gene position: 371916-371188 (Counterclockwise)

Preceding gene: 15644990

Following gene: 15644986

Centisome position: 22.3

GC content: 41.7

Gene sequence:

>729_bases
ATGCGTTGTTTTAAGGCTACTATCGCTTATGATGGGGCGTATTTTTTAGGCTATGCCAAACAGCCCAACAAACTCGGCGT
TCAAGACAAAATAGAGGACGCTTTAAATGCATTAGGGATTAAAAGCGTTGTGATTGCGGCCGGGCGCACGGATAAAGGCG
TGCATGCGAACAACCAGGTTTTGTCTTTTCACGCTCCAAAACACTGGAACGCCGCTAAATTATTTTATTACCTAGCCCCT
AAACTCGCCCCGCATATTGTCTTAAAAAAGCTAGAAGAAAAAAACTTCCATGCGCGTTTTGACGCTCAAAAAAGAGCGTA
TCGTTACCTTTTGACGAAGAATTTAAAAACGCCTTTTTTAGCGCCCTATATCGCTTGTGGGGATTATGGCTCACTAGACG
CATTAAACACCGCTTTAAAACAATTCACAGGCAAGCATGATTTTTCCATGTTTAAGAAAGAAGGCGGGGCTACAACCAAT
CCTAAACGCACTATTTTTAACGCTTTTGCTTATAAAACCTTTATCATAGGGCATGAGTGCGTGGTGTTTAAAATCATTGG
CGATGCATTTTTACGCTCTAGCGTGCGTTTGATCATTCAAGCATGCGTTCAATACTCCTTAGAAAAAATCACGCTCGCTG
AAATTCAAGCACAAATCCATAACCTCAAAGCCACTATAAGAACGCCCATAATGGCTAATGGCTTGTATTTGCACAGGGTG
TATTATTGA

Upstream 100 bases:

>100_bases
GATTTCTACGGATTTGTTTTTGATGACCTTTTTCTTCCCCTTTATTTGGGCGTTTGCCTCTTATTTGTTGTTTAGAAAAT
TCATTTTAAAGCGTTATTGA

Downstream 100 bases:

>100_bases
AACCTCAAAAGGTGTTCTTCCCAATCTAGAGCGCTTTTGATAATGGTGTCTAGGTTGTTATAAAGGGGTTTGAAAGAGGT
GTTTTGTAAGATTTTAGCGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIKSVVIAAGRTDKGVHANNQVLSFHAPKHWNAAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTN
PKRTIFNAFAYKTFIIGHECVVFKIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNLKATIRTPIMANGLYLHRV
YY

Sequences:

>Translated_242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIKSVVIAAGRTDKGVHANNQVLSFHAPKHWNAAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTN
PKRTIFNAFAYKTFIIGHECVVFKIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNLKATIRTPIMANGLYLHRV
YY
>Mature_242_residues
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIKSVVIAAGRTDKGVHANNQVLSFHAPKHWNAAKLFYYLAP
KLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIACGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTN
PKRTIFNAFAYKTFIIGHECVVFKIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNLKATIRTPIMANGLYLHRV
YY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=28.8617886178862, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27312; Mature: 27312

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIKSVVIAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCE
LSFHAPKHWNAAKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCC
APYIACGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTNPKRTIFNAFAYKTFIIGHEC
CCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCE
VVFKIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNLKATIRTPIMANGLYLHRV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEE
YY
EC
>Mature Secondary Structure
MRCFKATIAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIKSVVIAAGRTDKGVHANNQV
CCEEEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCE
LSFHAPKHWNAAKLFYYLAPKLAPHIVLKKLEEKNFHARFDAQKRAYRYLLTKNLKTPFL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCC
APYIACGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTNPKRTIFNAFAYKTFIIGHEC
CCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCE
VVFKIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNLKATIRTPIMANGLYLHRV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEE
YY
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA