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Identifier: 15644975

GI number: 15644975

Start: 353995

End: 354891

Strand: Reverse

Name: Not Available

Synonym: HP0347

Alternate gene names: 15644975

Gene position: 354891-353995 (Counterclockwise)

Preceding gene: 15644976

Following gene: 15644957

Centisome position: 21.28

GC content: 40.91

Gene sequence:

>897_bases
GTGCCTTTTGTTGAAGAAGAATTTGAAATTTTAAAACCCACCAAAGCCTTGTTTTTTGTGCGCGATGTTTTAAAATGCTC
TTTGAAAGAAGCCCAACGGCACCTTGACAAACAACGCCTGAAACAAAACCAACAAGCCGTGCGTAAATCTCAAATCATTC
AAGGGGTCGTTCGCTTGATTCATTTCAAGCCCAATGAAAAAACTCAAGCGCTTGTTTTTGAAACGAAAGATTTTGGCGTG
TTTGACAAACCCCATCAAGTCTATACCCACCCTAAAGGCTATTTTTACCATGAAAGCTTATTAGATTGTATCCAATCGCA
TTTTGGCAAAAACGCCCACCCAGCCCACAGACTAGACTATGAAACGAGCGGGTTGGTTTTAGCGGGCAAGACCTTACAAA
GCGTTAAGGATTTAAAAGCGCTTTTCATGCAAAAAAAAGTAAAAAAAACTTATTTAGCACTAGCGCATGGGTTGGTTGAA
AAAAGCATGAAAATAGACAAACCCATTCTAACGCCACAAAACATTCAAAAAGATTTGCACATTCGATCTAAAATTTCTCC
TTTAGGCAAGCAATCAATCACGCTTGTTGAGCCTTTAAGCTATAACCCTTTTTTGGATATAAGCCTACTCAAAATCACCC
CACTCACCGGGCGCACGCACCAGATCCGCTTGCATTTAAGCAGCGTGGATCATAGGATCGTGGGTGAGGGGCTTTATGGG
GTGGCAGATGAAAACGCTAGGGAGTATCTTCAATTAAAGCGTGAAAATAACGCCCCATTACTCATGCTCCATGCCGCTAG
TTTAGAGTTTGAATTTAAAGGAGCGATCTATAAAATCGCTTCCCCCATGCCTGAACGCTTCATGCCTTTTTTAAAAGATC
TATCCTTTTTTTATTGA

Upstream 100 bases:

>100_bases
AGCGGTTTTTGAAAGCGGGCGAAAAGGTGAGCGTGCTTTTTAGCGTGGAATTAGATGAGTGTTCTAATGAGCCTAAAATG
TTTGTTAAAAGTTTGTTGTA

Downstream 100 bases:

>100_bases
TTGATCTTTTGGTTGTCCTCTTCTGGGGTTTGGTTGAGGTCGCCTATATGTTCTTTGATGGTTTTTTTCTCCACCACAGG
AGCGTTTTTAAAACTCTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPFVEEEFEILKPTKALFFVRDVLKCSLKEAQRHLDKQRLKQNQQAVRKSQIIQGVVRLIHFKPNEKTQALVFETKDFGV
FDKPHQVYTHPKGYFYHESLLDCIQSHFGKNAHPAHRLDYETSGLVLAGKTLQSVKDLKALFMQKKVKKTYLALAHGLVE
KSMKIDKPILTPQNIQKDLHIRSKISPLGKQSITLVEPLSYNPFLDISLLKITPLTGRTHQIRLHLSSVDHRIVGEGLYG
VADENAREYLQLKRENNAPLLMLHAASLEFEFKGAIYKIASPMPERFMPFLKDLSFFY

Sequences:

>Translated_298_residues
MPFVEEEFEILKPTKALFFVRDVLKCSLKEAQRHLDKQRLKQNQQAVRKSQIIQGVVRLIHFKPNEKTQALVFETKDFGV
FDKPHQVYTHPKGYFYHESLLDCIQSHFGKNAHPAHRLDYETSGLVLAGKTLQSVKDLKALFMQKKVKKTYLALAHGLVE
KSMKIDKPILTPQNIQKDLHIRSKISPLGKQSITLVEPLSYNPFLDISLLKITPLTGRTHQIRLHLSSVDHRIVGEGLYG
VADENAREYLQLKRENNAPLLMLHAASLEFEFKGAIYKIASPMPERFMPFLKDLSFFY
>Mature_297_residues
PFVEEEFEILKPTKALFFVRDVLKCSLKEAQRHLDKQRLKQNQQAVRKSQIIQGVVRLIHFKPNEKTQALVFETKDFGVF
DKPHQVYTHPKGYFYHESLLDCIQSHFGKNAHPAHRLDYETSGLVLAGKTLQSVKDLKALFMQKKVKKTYLALAHGLVEK
SMKIDKPILTPQNIQKDLHIRSKISPLGKQSITLVEPLSYNPFLDISLLKITPLTGRTHQIRLHLSSVDHRIVGEGLYGV
ADENAREYLQLKRENNAPLLMLHAASLEFEFKGAIYKIASPMPERFMPFLKDLSFFY

Specific function: Unknown

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI23308689, Length=171, Percent_Identity=32.1637426900585, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI17158025, Length=236, Percent_Identity=25.8474576271186, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789155, Length=226, Percent_Identity=26.9911504424779, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788946, Length=184, Percent_Identity=27.1739130434783, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787327, Length=184, Percent_Identity=33.1521739130435, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786244, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321608, Length=185, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324506, Length=175, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320168, Length=177, Percent_Identity=30.5084745762712, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19921090, Length=233, Percent_Identity=29.6137339055794, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583449, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583447, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583445, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y347_HELPY (O25114)

Other databases:

- EMBL:   AE000511
- PIR:   C64563
- RefSeq:   NP_207145.1
- ProteinModelPortal:   O25114
- SMR:   O25114
- DIP:   DIP-3403N
- MINT:   MINT-169833
- GeneID:   899056
- GenomeReviews:   AE000511_GR
- KEGG:   hpy:HP0347
- NMPDR:   fig|85962.1.peg.343
- TIGR:   HP_0347
- HOGENOM:   HBG750656
- OMA:   NAREYLQ
- ProtClustDB:   CLSK872338
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 34348; Mature: 34216

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVEEEFEILKPTKALFFVRDVLKCSLKEAQRHLDKQRLKQNQQAVRKSQIIQGVVRLI
CCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFKPNEKTQALVFETKDFGVFDKPHQVYTHPKGYFYHESLLDCIQSHFGKNAHPAHRLDY
HCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
ETSGLVLAGKTLQSVKDLKALFMQKKVKKTYLALAHGLVEKSMKIDKPILTPQNIQKDLH
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
IRSKISPLGKQSITLVEPLSYNPFLDISLLKITPLTGRTHQIRLHLSSVDHRIVGEGLYG
HHHHCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCHHHCCCCCC
VADENAREYLQLKRENNAPLLMLHAASLEFEFKGAIYKIASPMPERFMPFLKDLSFFY
CCCCHHHHHHHHHCCCCCCEEEEEECCEEEEECCCEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFVEEEFEILKPTKALFFVRDVLKCSLKEAQRHLDKQRLKQNQQAVRKSQIIQGVVRLI
CCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFKPNEKTQALVFETKDFGVFDKPHQVYTHPKGYFYHESLLDCIQSHFGKNAHPAHRLDY
HCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
ETSGLVLAGKTLQSVKDLKALFMQKKVKKTYLALAHGLVEKSMKIDKPILTPQNIQKDLH
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
IRSKISPLGKQSITLVEPLSYNPFLDISLLKITPLTGRTHQIRLHLSSVDHRIVGEGLYG
HHHHCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCHHHCCCCCC
VADENAREYLQLKRENNAPLLMLHAASLEFEFKGAIYKIASPMPERFMPFLKDLSFFY
CCCCHHHHHHHHHCCCCCCEEEEEECCEEEEECCCEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185