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Identifier: 15644976

GI number: 15644976

Start: 354891

End: 356441

Strand: Reverse

Name: Not Available

Synonym: HP0348

Alternate gene names: 15644976

Gene position: 356441-354891 (Counterclockwise)

Preceding gene: 15644977

Following gene: 15644975

Centisome position: 21.37

GC content: 39.85

Gene sequence:

>1551_bases
ATGAAACAAAAGCTTAAAGCTCAAATCAAAGAGCGCATGGCTTCTATCGCTTATAATGAAAAAGGGTTTCCTAGCCCCTT
TTTATTTAAAGACTTGAAAAAAGCCGCGCTCAAAATCATAGAAGCCATGCGCACAAACACAGAAATTTTAGTGGTGGGCG
ATTATGACGCTGACGGCGTGATTAGCTCTGCTATCATGGCAAAATTTTTTGAAAGCCTGAACTATAAGCATGTCCGCATT
GCAATCCCTAATCGCTTCATGGATGGCTATGGGATTTCTAAAAAATTTTTAGAAAAACACCACGCCCCTTTAATCATCAC
GGTGGATAACGGGATTAACGCCTTTGAAGCCGCGCGATTTTGTAAAGAAAAAAATTACACCCTTATCATCACAGATCACC
ATTGCTTACACCATGATGAAGTCCCAGACGCTTATGCGGTGATCAACCCCAAGCAACCGGATTGTGATTTTATCCAAAAG
GAAGTGTGCGGGGCGTTGGTAGCGTTTTATTTGTGCTATGGGATCCATCAGCTTTTAGGAAAAGAAAAAAGCCATTCTAG
TGAGTTATTATGTTTAGCGGGCGTGGCGACTATTGCTGACATGATGCCTTTGACTTTTTTTAACCGCTTTTTAGTTTCTA
AAGCCTTGTATTTTTTGCAAAAAGAATCCTTAGGGGCGATGGGTTTTTTGCGCCAAAGAGAAGTTTTTAGAAAACGCTCT
TTAAAAGCGAGTGATATTTCTTTTAATATCGCCCCCTTAATCAACTCCGCAGGGCGCATGCAAGATGCGAAAATGGCTTT
AGATTTTTTAAGCGCGAATAATTCTCAAGATGGCTATTCTTTGTATGAACGCTTGAAAGCATGCAATTTGAAGCGTAAAA
TGATCCAACAGCAGGTTTTTGAAGAAGCTTTTAAGCATGCGATGGTTGGAGAAAAAATTATCGTCGCTTTTAAGGACAAT
TGGCATGAGGGAGTGCTGGGGATTGTGGCTTCAAAATTAGTGGAAGCCACTCAAAAGCCAAGCCTGGTTTTTACCTTTAA
AGAAGGGGTGTATAAAGGGAGCGCTCGTAGCTCTTCAAACATTGACTTGATTGACGCTTTGAATGGGGTTTCTTCTTTAT
TGCTCGGCTATGGAGGGCATAGGCAAGCTTGCGGGTTGAGCGTTGAAAAAAACAATATCATCTCGCTCTTTGAAACTTTA
GAAAATTTTGATTTTAAAGTTTTACCTTTTTGTAAAACAGAGCCCCCTTTAACGCTCAAATTAAAAGACATTGACAGAGA
GCTTTTAGAGATTATAGAAATGGGCGAACCTTATGGGCAAGAAAACCCTGAACCCCTATTCCAAGCAAAAAATTTAGAAG
TCATAGAAGAAAAAATCATTAAAGAAAGCCACCAGGTTTTGCGTTTTAAGGATAAAGAATGCGTCAAAGAGGCTATTTAT
TTTAGCGCTGAGCGGTTTTTGAAAGCGGGCGAAAAGGTGAGCGTGCTTTTTAGCGTGGAATTAGATGAGTGTTCTAATGA
GCCTAAAATGTTTGTTAAAAGTTTGTTGTAG

Upstream 100 bases:

>100_bases
GGGTGCAATTCCACCCAGAATTCACCTCCAGGTTGCAAAGCCCTAACCCTATTATTTTAGATTTCATTAAGAGCGCTCTT
TCTAAATCCTAATCTAACAA

Downstream 100 bases:

>100_bases
TGCCTTTTGTTGAAGAAGAATTTGAAATTTTAAAACCCACCAAAGCCTTGTTTTTTGTGCGCGATGTTTTAAAATGCTCT
TTGAAAGAAGCCCAACGGCA

Product: single-stranded-DNA-specific exonuclease (recJ)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKQKLKAQIKERMASIAYNEKGFPSPFLFKDLKKAALKIIEAMRTNTEILVVGDYDADGVISSAIMAKFFESLNYKHVRI
AIPNRFMDGYGISKKFLEKHHAPLIITVDNGINAFEAARFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFNIAPLINSAGRMQDAKMALDFLSANNSQDGYSLYERLKACNLKRKMIQQQVFEEAFKHAMVGEKIIVAFKDN
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSARSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVEKNNIISLFETL
ENFDFKVLPFCKTEPPLTLKLKDIDRELLEIIEMGEPYGQENPEPLFQAKNLEVIEEKIIKESHQVLRFKDKECVKEAIY
FSAERFLKAGEKVSVLFSVELDECSNEPKMFVKSLL

Sequences:

>Translated_516_residues
MKQKLKAQIKERMASIAYNEKGFPSPFLFKDLKKAALKIIEAMRTNTEILVVGDYDADGVISSAIMAKFFESLNYKHVRI
AIPNRFMDGYGISKKFLEKHHAPLIITVDNGINAFEAARFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFNIAPLINSAGRMQDAKMALDFLSANNSQDGYSLYERLKACNLKRKMIQQQVFEEAFKHAMVGEKIIVAFKDN
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSARSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVEKNNIISLFETL
ENFDFKVLPFCKTEPPLTLKLKDIDRELLEIIEMGEPYGQENPEPLFQAKNLEVIEEKIIKESHQVLRFKDKECVKEAIY
FSAERFLKAGEKVSVLFSVELDECSNEPKMFVKSLL
>Mature_516_residues
MKQKLKAQIKERMASIAYNEKGFPSPFLFKDLKKAALKIIEAMRTNTEILVVGDYDADGVISSAIMAKFFESLNYKHVRI
AIPNRFMDGYGISKKFLEKHHAPLIITVDNGINAFEAARFCKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQK
EVCGALVAFYLCYGIHQLLGKEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
LKASDISFNIAPLINSAGRMQDAKMALDFLSANNSQDGYSLYERLKACNLKRKMIQQQVFEEAFKHAMVGEKIIVAFKDN
WHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSARSSSNIDLIDALNGVSSLLLGYGGHRQACGLSVEKNNIISLFETL
ENFDFKVLPFCKTEPPLTLKLKDIDRELLEIIEMGEPYGQENPEPLFQAKNLEVIEEKIIKESHQVLRFKDKECVKEAIY
FSAERFLKAGEKVSVLFSVELDECSNEPKMFVKSLL

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=470, Percent_Identity=31.2765957446809, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 58354; Mature: 58354

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKLKAQIKERMASIAYNEKGFPSPFLFKDLKKAALKIIEAMRTNTEILVVGDYDADGV
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
ISSAIMAKFFESLNYKHVRIAIPNRFMDGYGISKKFLEKHHAPLIITVDNGINAFEAARF
HHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
CKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQKEVCGALVAFYLCYGIHQLLG
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LKASDISFNIAPLINSAGRMQDAKMALDFLSANNSQDGYSLYERLKACNLKRKMIQQQVF
CCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEAFKHAMVGEKIIVAFKDNWHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSARSSSN
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
IDLIDALNGVSSLLLGYGGHRQACGLSVEKNNIISLFETLENFDFKVLPFCKTEPPLTLK
CHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCEEEE
LKDIDRELLEIIEMGEPYGQENPEPLFQAKNLEVIEEKIIKESHQVLRFKDKECVKEAIY
EHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FSAERFLKAGEKVSVLFSVELDECSNEPKMFVKSLL
HHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MKQKLKAQIKERMASIAYNEKGFPSPFLFKDLKKAALKIIEAMRTNTEILVVGDYDADGV
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHH
ISSAIMAKFFESLNYKHVRIAIPNRFMDGYGISKKFLEKHHAPLIITVDNGINAFEAARF
HHHHHHHHHHHHCCCCEEEEEECCHHCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
CKEKNYTLIITDHHCLHHDEVPDAYAVINPKQPDCDFIQKEVCGALVAFYLCYGIHQLLG
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKSHSSELLCLAGVATIADMMPLTFFNRFLVSKALYFLQKESLGAMGFLRQREVFRKRS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LKASDISFNIAPLINSAGRMQDAKMALDFLSANNSQDGYSLYERLKACNLKRKMIQQQVF
CCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EEAFKHAMVGEKIIVAFKDNWHEGVLGIVASKLVEATQKPSLVFTFKEGVYKGSARSSSN
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
IDLIDALNGVSSLLLGYGGHRQACGLSVEKNNIISLFETLENFDFKVLPFCKTEPPLTLK
CHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCEEEE
LKDIDRELLEIIEMGEPYGQENPEPLFQAKNLEVIEEKIIKESHQVLRFKDKECVKEAIY
EHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FSAERFLKAGEKVSVLFSVELDECSNEPKMFVKSLL
HHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]