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Identifier: 15644888

GI number: 15644888

Start: 269378

End: 270532

Strand: Reverse

Name: Not Available

Synonym: HP0260

Alternate gene names: 15644888

Gene position: 270532-269378 (Counterclockwise)

Preceding gene: 15644889

Following gene: 15644879

Centisome position: 16.22

GC content: 32.21

Gene sequence:

>1155_bases
TTGCATAAAGTTTTTATCATGGAAGCTTTGGAGTGTTTGAAAAGGATAGAAAAAGAAAGCATCCAAACGATCTATATAGA
CCCCCCTTATAACACTAAGAGTTCTAACTTTGAATATGAAGACGATCATGCTGACTATGAAAAATGGATTAAAGAACATT
TGATTTTAGCAAAAGCTGTGTTAAAACAAAGCGGTTGTCTTTTTATTTCTATGGACGATAACAAAATGGCTGAAGTTAAA
ATCATTGCCAATGAGATTTTTGGAACGCGCAATTTTTTAGGCACTTTTATCACTAAACAAGCCACAAGATCTAACGCTAA
ACACATCAATATTACTCATGAATATGTTTTAAGCTACGCTAAAAATAAAGCGTTCGCTCCTGGTTTTAAAATCTTACGAA
CGCTTTTGCCCATTTATGCTAGAGCATTAAAAGATTTAATGCGAACGATTAAGAATGTTTTCAAACAAAAAGGACAAGCT
CAAGCCCAACTTGTCTTAAAAGAACAAATCAAAGAGTTATCCCAAAAAGAACATTTTAATTTTTTAAAAAATTATAATTT
AGTGGATGAAAAAGGTGAGATTTATTTCGCTAAAGATTTATCCACGCCTTCAAACCCACGCAGTGTAGCGATAGAAGAAA
TCAATCTTTTTTTAGAACCCTTAAAAAGCAGAGGGTGGAGCAGCGATGAAAAGCTTAAGGAGTTATATTATCAAAACAGA
CTTATTTTTAAGAATAATCGCCCTTATGAAAAATATTACCTAAAAGAATCGCAAGATAATTGTTTGAGCGTGTTGGATTT
TTATAGCCGACAAGGCACAAAAGATTTAGAAAAATTAGGCTTAAAGGGGCTTTTTAAGACGCCAAAACCTGTAGCATTGA
TTAAATATTTATTGCTATGCTCCACCCCCAAAGATTCTATTATTTTAGATTTTTTTGCAGGCAGCGGGACAACAGCGCAA
GCGGTTATAGAAGTTAATAGGGATTATTGTTTGAATTGGTCTTTTTATTTGTGTCAAAAAGAAGAAAAAATTAAAAATAA
CCCGCAAGCTGTTAGTATTTTAAAAAATAAGGGGTATCAAAACACGATTTCAAACATCATGCTATTGCGTTTAGAAAAGA
TCATTAAAAGAAGTGAATACGAAATTTTAAGATAA

Upstream 100 bases:

>100_bases
AAAGAATTTGAATTTAAAATGAAACCCATTGATTCAAAAGAACAAGAGCTTATCAATCTTTTTAATCTCAAGTTTGGTCA
TTTTTCTTGGGAAAATTATC

Downstream 100 bases:

>100_bases
AACAAATAGCGTTTTAAATATTATTTTTCAAACCCCTACACCCTATCCACGCTCAAAATTTCAGCGCTCGCTCTGGTTTT
TTCATTACTTAATTCAATTT

Product: adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.EcoP15I; EcoP15I methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MHKVFIMEALECLKRIEKESIQTIYIDPPYNTKSSNFEYEDDHADYEKWIKEHLILAKAVLKQSGCLFISMDDNKMAEVK
IIANEIFGTRNFLGTFITKQATRSNAKHINITHEYVLSYAKNKAFAPGFKILRTLLPIYARALKDLMRTIKNVFKQKGQA
QAQLVLKEQIKELSQKEHFNFLKNYNLVDEKGEIYFAKDLSTPSNPRSVAIEEINLFLEPLKSRGWSSDEKLKELYYQNR
LIFKNNRPYEKYYLKESQDNCLSVLDFYSRQGTKDLEKLGLKGLFKTPKPVALIKYLLLCSTPKDSIILDFFAGSGTTAQ
AVIEVNRDYCLNWSFYLCQKEEKIKNNPQAVSILKNKGYQNTISNIMLLRLEKIIKRSEYEILR

Sequences:

>Translated_384_residues
MHKVFIMEALECLKRIEKESIQTIYIDPPYNTKSSNFEYEDDHADYEKWIKEHLILAKAVLKQSGCLFISMDDNKMAEVK
IIANEIFGTRNFLGTFITKQATRSNAKHINITHEYVLSYAKNKAFAPGFKILRTLLPIYARALKDLMRTIKNVFKQKGQA
QAQLVLKEQIKELSQKEHFNFLKNYNLVDEKGEIYFAKDLSTPSNPRSVAIEEINLFLEPLKSRGWSSDEKLKELYYQNR
LIFKNNRPYEKYYLKESQDNCLSVLDFYSRQGTKDLEKLGLKGLFKTPKPVALIKYLLLCSTPKDSIILDFFAGSGTTAQ
AVIEVNRDYCLNWSFYLCQKEEKIKNNPQAVSILKNKGYQNTISNIMLLRLEKIIKRSEYEILR
>Mature_384_residues
MHKVFIMEALECLKRIEKESIQTIYIDPPYNTKSSNFEYEDDHADYEKWIKEHLILAKAVLKQSGCLFISMDDNKMAEVK
IIANEIFGTRNFLGTFITKQATRSNAKHINITHEYVLSYAKNKAFAPGFKILRTLLPIYARALKDLMRTIKNVFKQKGQA
QAQLVLKEQIKELSQKEHFNFLKNYNLVDEKGEIYFAKDLSTPSNPRSVAIEEINLFLEPLKSRGWSSDEKLKELYYQNR
LIFKNNRPYEKYYLKESQDNCLSVLDFYSRQGTKDLEKLGLKGLFKTPKPVALIKYLLLCSTPKDSIILDFFAGSGTTAQ
AVIEVNRDYCLNWSFYLCQKEEKIKNNPQAVSILKNKGYQNTISNIMLLRLEKIIKRSEYEILR

Specific function: Binds the system-specific DNA recognition site 5'- CAGCAC-3'. Necessary for both restriction and methylation (of one of the two A's) [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 44774; Mature: 44774

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVFIMEALECLKRIEKESIQTIYIDPPYNTKSSNFEYEDDHADYEKWIKEHLILAKAV
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LKQSGCLFISMDDNKMAEVKIIANEIFGTRNFLGTFITKQATRSNAKHINITHEYVLSYA
HHHCCEEEEEECCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
KNKAFAPGFKILRTLLPIYARALKDLMRTIKNVFKQKGQAQAQLVLKEQIKELSQKEHFN
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FLKNYNLVDEKGEIYFAKDLSTPSNPRSVAIEEINLFLEPLKSRGWSSDEKLKELYYQNR
HHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LIFKNNRPYEKYYLKESQDNCLSVLDFYSRQGTKDLEKLGLKGLFKTPKPVALIKYLLLC
EEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
STPKDSIILDFFAGSGTTAQAVIEVNRDYCLNWSFYLCQKEEKIKNNPQAVSILKNKGYQ
CCCCCCEEEEEECCCCCCEEEEEEECCCEEECCEEEEECCHHHHCCCCHHHHHHHCCCCH
NTISNIMLLRLEKIIKRSEYEILR
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHKVFIMEALECLKRIEKESIQTIYIDPPYNTKSSNFEYEDDHADYEKWIKEHLILAKAV
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LKQSGCLFISMDDNKMAEVKIIANEIFGTRNFLGTFITKQATRSNAKHINITHEYVLSYA
HHHCCEEEEEECCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
KNKAFAPGFKILRTLLPIYARALKDLMRTIKNVFKQKGQAQAQLVLKEQIKELSQKEHFN
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
FLKNYNLVDEKGEIYFAKDLSTPSNPRSVAIEEINLFLEPLKSRGWSSDEKLKELYYQNR
HHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LIFKNNRPYEKYYLKESQDNCLSVLDFYSRQGTKDLEKLGLKGLFKTPKPVALIKYLLLC
EEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
STPKDSIILDFFAGSGTTAQAVIEVNRDYCLNWSFYLCQKEEKIKNNPQAVSILKNKGYQ
CCCCCCEEEEEECCCCCCEEEEEEECCCEEECCEEEEECCHHHHCCCCHHHHHHHCCCCH
NTISNIMLLRLEKIIKRSEYEILR
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837577 [H]