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Identifier: 15644866

GI number: 15644866

Start: 247560

End: 249293

Strand: Reverse

Name: Not Available

Synonym: HP0238

Alternate gene names: 15644866

Gene position: 249293-247560 (Counterclockwise)

Preceding gene: 15644867

Following gene: 15644865

Centisome position: 14.95

GC content: 40.89

Gene sequence:

>1734_bases
ATGCTATTTTCAAAACTCTTTGCCCCCACTCTCAAAGAACCCCCTAAAGATGCCGTGTTAAAAAGCCATAAACACTTAGC
TCAAGCAGGATACATTTATCAAGTAGGCAGCGGGATTTATAATTTTTTGCCTTTAGCTAAAAAAGTGCTAGACAAGATAG
AAAACATCACGCACAAACGCATGCAAGAGCATGGGGCGCAAAATATTTTAATGAGTTTTGTGGTTTTGGCGAGCTTGTGG
GAAAAATCAGGCCGTTTGGATAAATACGGCAAGGAATTATTGGTTTTTAAAGACCGAAAAGACAATGATTTTGTTTTAAG
CCCCACTTTAGAAGAAAATATCACCGAAATTGCCGCTAATTTCATTAAAAGTTACAAGCAATTGCCCGTCCATCTCTATC
AAATCCACACGAAATTCCGTGATGAAATCCGCCCAAGATTTGGGCTGGTGAGAGCGAGGGAATTTATCATGAAAGATGGT
TACAGCTTTCATGAAGACGCTGAGAGCTTGGATAAGGAATTTTTAAACACGCAAAGCGCTTATAAAGAGATTTTAAGCGA
TTTGGGTTTGGATTTTCGCATTGTGGAAGCGGATAGCGGGGCGATTGGAGGGAGTAAAAGCAGGGAATTTGTCGTTTTAA
CCGAATGCGGGGAAGACACGATCGTGGTGTGTCAAAATTGCGATTATGCCGCTAACATTGAAATCGCTAAACGCTCTAAA
AGGCCTGAGCCTTTAAATGTCCCAAAAGCGCAATTAGCGAAATTCCCTACCCCTAATACCACTAGCGCGCAAAGCGTGGC
GGAGTTTTTTAAAACAGAGCCTTATTTTGTCTTAAAAGCGCTTGTTAGAAAAGTGATCCATAAAGATAAAGAAACCCTAG
CGTGCTTTTTTGTCAGGGGCGATGACAATTTAGAGGAAGTTAAAGCCCTAAACGCCTTGAACATTATAGGAGCGAATGCT
TTAGAATTGAGAGAGGCGAGTCAAAAAGATTTGGATAATGTGGGGTTAATAGCGGGTTTTATAGGGCCTTATGGCTTGAA
AAAGCATGTTTCTTACATTATTTTTGATGAAGATTTAAAAGAGGGCGATTGCTTGATCGCTGGGGCTAATGAAAAGGATT
TTCATGCGGTGGGCGTGGATTTAAAAGGGTTTGAAAATCTTGTTTATGCGGATATTGTCCAGGTTAAAGAGAGCGATCGT
TGCCCTAATTGTCAAGGAGCGTTGAAATACCATAAGAGTTTGGAGGTGGGGCATATTTTCAAACTCGGGCAAGGCTATGC
TAAAAGCTTGAAGGCTAGTTTCTTGGATAAGAATGGTAAAGAGCAATTTTTTGAAATGGGGTGCTATGGGATAGGCATTA
GCCGGTTGCTCAGCGCGATTTTAGAGCAAAAAAGCGATGATTTAGGCTGTGTGTGGACGAAAAATACCGCCCCTTTTGAT
GTGGTGATCGTGGTTTCTAATTGGAAAGATGAAGCGCAAAAAAAACTCGCTTTTGAAGTGTATGAAAGGCTGCTCCAAAA
GGGCGTTGATGCGCTGCTAGATGACAGAGACGCGCGTTTTGGGGCGAAGATGAGGGATTTTGAATTGATTGGGGAACGAT
TGGCGCTCATTATTGGGAAGCAAACTTTAGAGAGCAAAGAATTTGAATGCATCAAACGCGCTAATTTGGAAAAACAAACG
ATTAAAGACATTGAATTAGAAGAAAAAATTTTAGAAATGTTAGAAAGCGAATAA

Upstream 100 bases:

>100_bases
TGTGGGTGCGATTAAAAACTTGTTTAATTTAGACAAATCTAACGCTAACCATGCCCAGAATTTGAATCTCTATAAATGCG
AATATTACGAGGAATAATGC

Downstream 100 bases:

>100_bases
GGGGGAGTGAGTGGGAAATTTAGTGATTGGCTCTAGGGGGAGCGAATTAGCCTTATGGCAAGCGAATCACATTAAAGAAC
GCCTGAAAAAAGAATGCTTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILSDLGLDFRIVEADSGAIGGSKSREFVVLTECGEDTIVVCQNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDNVGLIAGFIGPYGLKKHVSYIIFDEDLKEGDCLIAGANEKDFHAVGVDLKGFENLVYADIVQVKESDR
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLKASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLESKEFECIKRANLEKQT
IKDIELEEKILEMLESE

Sequences:

>Translated_577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILSDLGLDFRIVEADSGAIGGSKSREFVVLTECGEDTIVVCQNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDNVGLIAGFIGPYGLKKHVSYIIFDEDLKEGDCLIAGANEKDFHAVGVDLKGFENLVYADIVQVKESDR
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLKASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLESKEFECIKRANLEKQT
IKDIELEEKILEMLESE
>Mature_577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILSDLGLDFRIVEADSGAIGGSKSREFVVLTECGEDTIVVCQNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDNVGLIAGFIGPYGLKKHVSYIIFDEDLKEGDCLIAGANEKDFHAVGVDLKGFENLVYADIVQVKESDR
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLKASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLESKEFECIKRANLEKQT
IKDIELEEKILEMLESE

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=243, Percent_Identity=39.917695473251, Blast_Score=188, Evalue=1e-47,
Organism=Escherichia coli, GI1786392, Length=578, Percent_Identity=39.7923875432526, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=41.4285714285714, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=37.6470588235294, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=534, Percent_Identity=31.6479400749064, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24656200, Length=242, Percent_Identity=37.603305785124, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 65224; Mature: 65224

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKR
CCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEHGAQNILMSFVVLASLWEKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAAN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
FIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDGYSFHEDAESLDKEFLNTQSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKEILSDLGLDFRIVEADSGAIGGSKSREFVVLTECGEDTIVVCQNCDYAANIEIAKRSK
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
DDNLEEVKALNALNIIGANALELREASQKDLDNVGLIAGFIGPYGLKKHVSYIIFDEDLK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCEEEEEECCCC
EGDCLIAGANEKDFHAVGVDLKGFENLVYADIVQVKESDRCPNCQGALKYHKSLEVGHIF
CCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHH
KLGQGYAKSLKASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
HHHHHHHHHHHHHHHCCCCHHHHHHHCHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHH
QTLESKEFECIKRANLEKQTIKDIELEEKILEMLESE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKR
CCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEHGAQNILMSFVVLASLWEKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAAN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
FIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDGYSFHEDAESLDKEFLNTQSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKEILSDLGLDFRIVEADSGAIGGSKSREFVVLTECGEDTIVVCQNCDYAANIEIAKRSK
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
DDNLEEVKALNALNIIGANALELREASQKDLDNVGLIAGFIGPYGLKKHVSYIIFDEDLK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCEEEEEECCCC
EGDCLIAGANEKDFHAVGVDLKGFENLVYADIVQVKESDRCPNCQGALKYHKSLEVGHIF
CCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHH
KLGQGYAKSLKASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
HHHHHHHHHHHHHHHCCCCHHHHHHHCHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHH
QTLESKEFECIKRANLEKQTIKDIELEEKILEMLESE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA