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The map label for this gene is ruvB [H]

Identifier: 13508275

GI number: 13508275

Start: 657517

End: 658440

Strand: Direct

Name: ruvB [H]

Synonym: MPN536

Alternate gene names: 13508275

Gene position: 657517-658440 (Clockwise)

Preceding gene: 13508274

Following gene: 13508277

Centisome position: 80.54

GC content: 41.88

Gene sequence:

>924_bases
ATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAG
CATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGTGTTGGTAAAACCACAT
TAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTT
CTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGA
GTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGA
AAGTTAATCCGTTTACCTGAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATG
ATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATT
AAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGT
TTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAG
AGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGG
GATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGC
GGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAA

Upstream 100 bases:

>100_bases
CAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAA
GTTAATGTCTTTCCAGAACA

Downstream 100 bases:

>100_bases
CTTTTTGGCACGCTGGATAATCTCATAACCAAAGCTTTGGTTAATCTCATAGATCATTTGATCTAAAGCAAAGTGAGCAG
TCTGTTGTTTAGGCTTAGCT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDF
LNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFTWIGATTQFGKIINPLEDRFGM
ILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQ
SIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC

Sequences:

>Translated_307_residues
MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDF
LNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFT*IGATTQFGKIINPLEDRFGM
ILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQ
SIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC
>Mature_307_residues
MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDF
LNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFT*IGATTQFGKIINPLEDRFGM
ILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQ
SIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=304, Percent_Identity=31.9078947368421, Blast_Score=197, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF00004 AAA; PF05491 RuvB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 34809; Mature: 34809

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIAS
CEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHH
EMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQV
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEE
LIGKDFNSKMVEMKVNPFTIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGE
EEECCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCC
QMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQS
CEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
IDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGR
HHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCC
NYLTSC
HHHCCC
>Mature Secondary Structure
MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIAS
CEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCHHHHHHHHHH
EMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQV
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEE
LIGKDFNSKMVEMKVNPFTIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGE
EEECCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCC
QMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQS
CEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
IDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGR
HHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCC
NYLTSC
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]