Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 13508141

GI number: 13508141

Start: 483529

End: 484980

Strand: Direct

Name: proS [H]

Synonym: MPN402

Alternate gene names: 13508141

Gene position: 483529-484980 (Clockwise)

Preceding gene: 13508136

Following gene: 13508142

Centisome position: 59.23

GC content: 42.29

Gene sequence:

>1452_bases
ATGGCCAATAAAGATCAAAATCTAACGCTTTGATACGACCAACTACTGAGCAAAGCACAATTAGTGAGCTATGGCGATGT
GAAGGGCACTAACTGTTTTTTACCCAACAGTTGGAACCTCTGACTACAAATTCAAAGACTCTATAATAACGCTACTGCTC
TAATTAAATTAAAGGACAAGGTTATTTTAAAGCAGTTTATCCCGATTGAACCACTGCCATACACAGTGGAGCAAGTTCAA
CTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAAT
AGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGA
AAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGAACCTGTGTTTTTCGCGCTGAAAAAAACACC
CGCCCTTTCTTGCGCAACAGTGAGTTTTACTGACAAGAAACCCACGGTTTGTTTAGTGATGGAGTTAATTCTGAAAGCGC
AGCAATTGCTTTTTGAAAACTGTACCAGGACATTATTGTCAACCAGCTCTGTATCCCCGCTTTTGTGGGGTTAAAGAGTC
CCAATGAGCGCTTTGCCGGAGCACAAAATACCTGAACGGTGGAAAGCATTATGCCCGATGGGCAGGCATTGCAGTGTGCC
ACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCC
CTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTGATGACTTTGGACTGGTAC
TACCATGAAAAGTAGCACCAATTCAAGTCAAACTGTACTTATTTGACAAAAAAGGTGATACAAAGACAGTAGAGTTAGCA
CAGAAAGTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTT
AGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAA
CCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATG
TATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGA
CAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGC
GCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCG
CGTGCCTATTAA

Upstream 100 bases:

>100_bases
TTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGC
TAACCAATAAAACGTAGTTG

Downstream 100 bases:

>100_bases
TAACTTTTTACGAAAACTTTTTGCTTAAAAGCAAACCCTAAATGTAAAGGCTAAAAAGGTGGTTTTTATTTTCCACTTCT
GGTCGTTTTTTGTCAATAAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MANKDQNLTLWYDQLLSKAQLVSYGDVKGTNCFLPNSWNLWLQIQRLYNNATALIKLKDKVILKQFIPIEPLPYTVEQVQ
LPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQWTCVFRAEKNT
RPFLRNSEFYWQETHGLFSDGVNSESAAIAFWKLYQDIIVNQLCIPAFVGLKSPNERFAGAQNTWTVESIMPDGQALQCA
TSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLPWKVAPIQVKLYLFDKKGDTKTVELA
QKVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAM
YDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIA
RAY

Sequences:

>Translated_483_residues
MANKDQNLTL*YDQLLSKAQLVSYGDVKGTNCFLPNSWNL*LQIQRLYNNATALIKLKDKVILKQFIPIEPLPYTVEQVQ
LPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQ*TCVFRAEKNT
RPFLRNSEFY*QETHGLFSDGVNSESAAIAF*KLYQDIIVNQLCIPAFVGLKSPNERFAGAQNT*TVESIMPDGQALQCA
TSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLP*KVAPIQVKLYLFDKKGDTKTVELA
QKVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAM
YDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIA
RAY
>Mature_482_residues
ANKDQNLTL*YDQLLSKAQLVSYGDVKGTNCFLPNSWNL*LQIQRLYNNATALIKLKDKVILKQFIPIEPLPYTVEQVQL
PTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQ*TCVFRAEKNTR
PFLRNSEFY*QETHGLFSDGVNSESAAIAF*KLYQDIIVNQLCIPAFVGLKSPNERFAGAQNT*TVESIMPDGQALQCAT
SHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLP*KVAPIQVKLYLFDKKGDTKTVELAQ
KVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMY
DKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIAR
AY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=445, Percent_Identity=27.8651685393258, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71991342, Length=510, Percent_Identity=27.843137254902, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71991346, Length=367, Percent_Identity=30.7901907356948, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321808, Length=454, Percent_Identity=28.4140969162996, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24649468, Length=416, Percent_Identity=30.7692307692308, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24649466, Length=416, Percent_Identity=30.7692307692308, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54026; Mature: 53895

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00108 PROTEIN_KINASE_ST ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKDQNLTLYDQLLSKAQLVSYGDVKGTNCFLPNSWNLLQIQRLYNNATALIKLKDKVI
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEHHHHH
LKQFIPIEPLPYTVEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLR
HHHCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEC
PTSEIAFCNLWKKQSFSYQNLPVIYNQTCVFRAEKNTRPFLRNSEFYQETHGLFSDGVNS
CCCCHHHHHHHHHCCCCCCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ESAAIAFKLYQDIIVNQLCIPAFVGLKSPNERFAGAQNTTVESIMPDGQALQCATSHDLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCCCEEEEECCCCHH
QTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLPKVAPIQVKLYL
HHHCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEE
FDKKGDTKTVELAQKVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
NIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
ANKDQNLTLYDQLLSKAQLVSYGDVKGTNCFLPNSWNLLQIQRLYNNATALIKLKDKVI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEHHHHH
LKQFIPIEPLPYTVEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLR
HHHCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEC
PTSEIAFCNLWKKQSFSYQNLPVIYNQTCVFRAEKNTRPFLRNSEFYQETHGLFSDGVNS
CCCCHHHHHHHHHCCCCCCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ESAAIAFKLYQDIIVNQLCIPAFVGLKSPNERFAGAQNTTVESIMPDGQALQCATSHDLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCCCEEEEECCCCHH
QTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLPKVAPIQVKLYL
HHHCCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEECCCCCCEEEEEEE
FDKKGDTKTVELAQKVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
NIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]