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The map label for this gene is spoT [H]

Identifier: 13508136

GI number: 13508136

Start: 476470

End: 478671

Strand: Direct

Name: spoT [H]

Synonym: MPN397

Alternate gene names: 13508136

Gene position: 476470-478671 (Clockwise)

Preceding gene: 13508121

Following gene: 13508141

Centisome position: 58.36

GC content: 36.6

Gene sequence:

>2202_bases
ATGTTTTACAATTGACTTAAATTATATAAATTTAGCAAAATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAA
AACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGACACGGTGAGC
AAAAAAGGCTGAGTGGTGAACCGTTTTTTATCCATCCCTTGAGAACAGCACTGCGCTTAGTGGAGTGAAACATGGACAGC
AACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGG
TAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGA
AAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTG
GCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGA
ACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAG
ATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGGCAACTTTAAA
AAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTA
TCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGT
TGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCC
CCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCA
ACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTG
CTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATT
AAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATAT
TGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACG
TAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATG
AATAACCACGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCT
ATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGA
TCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGT
AACAATCAGAAGTGAAGGGAATTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGA
ACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTA
TTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATT
ATTCCACTTTATTGAAATGCACACCAATTAAAGATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATG
GAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCG
ATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAG
ATTACTACTAAGAATATTAGCTACGTTTGAAAGTACCTTTAA

Upstream 100 bases:

>100_bases
TACCAAAAATAATTCCAAACAGGCCCAAAAAAGCTAAGACCAGGATAATTCCAATCTTTAAGAGCCAGTCAAAGGAAACC
CGCTTTTTCAGTCCCACTGG

Downstream 100 bases:

>100_bases
CTTTCAAATACTTCGTCATAAACATCTTGGTTCTTTTGTAAAAGTAACACTAAGTGTTTCTTCAATAGCCTTTGTGGGTT
AAAGCGCGATACTCGTACCC

Product: p)ppGpp 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MFYNWLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKWHGEQKRLSGEPFFIHPLRTALRLVEWNMDS
NTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKL
ADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFK
KQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIAS
PKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDII
KVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYM
NNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLAS
NNQKWRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKI
IPLYWNAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQ
ITTKNISYVWKYL

Sequences:

>Translated_733_residues
MFYN*LKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKK*HGEQKRLSGEPFFIHPLRTALRLVE*NMDS
NTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKL
ADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFK
KQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIAS
PKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDII
KVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYM
NNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLAS
NNQK*RELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKI
IPLY*NAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQ
ITTKNISYV*KYL
>Mature_733_residues
MFYN*LKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKK*HGEQKRLSGEPFFIHPLRTALRLVE*NMDS
NTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKL
ADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFK
KQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIAS
PKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDII
KVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYM
NNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLAS
NNQK*RELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKI
IPLY*NAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQ
ITTKNISYV*KYL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=601, Percent_Identity=27.4542429284526, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI1789147, Length=584, Percent_Identity=25, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811 [H]

Pfam domain/function: PF04607 RelA_SpoT [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 85180; Mature: 85180

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYNLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKHGEQKRLSGEPF
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
FIHPLRTALRLVENMDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAK
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
ETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQL
HHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHCCCEECCCCCHHHHHH
EEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECEEEEEEEHHHHHHHHHCCCHHHHHH
KEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSK
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC
LLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNH
HHHHHHHHHHHHHCHHHHEEECCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
VFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLEL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IEYPHFPKNEHLYFLASNNQKRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCT
HCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLYNAHQLKMRPRKFRCQINIRG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCEEEEEEEEEE
VWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKN
ECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCC
ISYVKYL
CCCEECC
>Mature Secondary Structure
MFYNLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKHGEQKRLSGEPF
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
FIHPLRTALRLVENMDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAK
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
ETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQL
HHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHCCCEECCCCCHHHHHH
EEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECEEEEEEEHHHHHHHHHCCCHHHHHH
KEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSK
HHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC
LLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNH
HHHHHHHHHHHHHCHHHHEEECCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHH
VFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLEL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IEYPHFPKNEHLYFLASNNQKRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCT
HCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLYNAHQLKMRPRKFRCQINIRG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCEEEEEEEEEE
VWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKN
ECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCC
ISYVKYL
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]