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The map label for this gene is polA [H]

Identifier: 13508118

GI number: 13508118

Start: 456272

End: 457147

Strand: Direct

Name: polA [H]

Synonym: MPN379

Alternate gene names: 13508118

Gene position: 456272-457147 (Clockwise)

Preceding gene: 13508117

Following gene: 13508119

Centisome position: 55.89

GC content: 37.21

Gene sequence:

>876_bases
ATGAAAAATGCAATTTTAATTGATGGTAACTCACTAGCCTACCGTGCTTACTTCGCTACTTGACAACAGGTGGAGTTTGC
CAAACTACACAACTTACCCTTTAACAATGCGATTCGCACAATGTTAATGATGTGCTGAAACTTACTGCAGTCAAAACAGT
ATGACTATGGTGTTATTAGCTTTGATACTAAAGCGCCCACTTTTCGTGATCAACTTTACAGTGAATACAAAAGTAAGCGC
AGTAAAACACCGAGTGAACTGTTAGTCCAAATTCCTGTTGTTAAAGAATCACTGCGTCATTTAGGTTTTTTAGTGTGTGA
ACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCT
ACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATG
AAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGT
AGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTA
CCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCT
AAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAAT
CGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAA

Upstream 100 bases:

>100_bases
GGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGAA
AGAGCTTTGGTTTTTAATTT

Downstream 100 bases:

>100_bases
TGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAG
GTTCACTTAGACAAGGTGTT

Product: 5'-3' exonuclease (complete)

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKNAILIDGNSLAYRAYFATWQQVEFAKLHNLPFNNAIRTMLMMCWNLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKR
SKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEM
KEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTA
KKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL

Sequences:

>Translated_291_residues
MKNAILIDGNSLAYRAYFAT*QQVEFAKLHNLPFNNAIRTMLMMC*NLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKR
SKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEM
KEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTA
KKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL
>Mature_291_residues
MKNAILIDGNSLAYRAYFAT*QQVEFAKLHNLPFNNAIRTMLMMC*NLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKR
SKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEM
KEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTA
KKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=294, Percent_Identity=29.9319727891156, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI226510970, Length=219, Percent_Identity=26.4840182648402, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32564; Mature: 32564

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAILIDGNSLAYRAYFATQQVEFAKLHNLPFNNAIRTMLMMCNLLQSKQYDYGVISFD
CCCCEEEECCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
TKAPTFRDQLYSEYKSKRSKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYAR
CCCCHHHHHHHHHHHHHHCCCHHHHHEECHHHHHHHHHHCEEEECCCCCCHHHHHHHHHH
LMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIE
HHHCCCEEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHHCCCHHHHHHHHCCCHHHHHH
YKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKK
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
FSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL
HHHHHHHHHCCEECHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCC
>Mature Secondary Structure
MKNAILIDGNSLAYRAYFATQQVEFAKLHNLPFNNAIRTMLMMCNLLQSKQYDYGVISFD
CCCCEEEECCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
TKAPTFRDQLYSEYKSKRSKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYAR
CCCCHHHHHHHHHHHHHHCCCHHHHHEECHHHHHHHHHHCEEEECCCCCCHHHHHHHHHH
LMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIE
HHHCCCEEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHHCCCHHHHHHHHCCCHHHHHH
YKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKK
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
FSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL
HHHHHHHHHCCEECHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]