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The map label for this gene is prfA [H]

Identifier: 13508100

GI number: 13508100

Start: 430628

End: 431707

Strand: Direct

Name: prfA [H]

Synonym: MPN361

Alternate gene names: 13508100

Gene position: 430628-431707 (Clockwise)

Preceding gene: 13508099

Following gene: 13508101

Centisome position: 52.75

GC content: 42.59

Gene sequence:

>1080_bases
GTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAA
ACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAG
AATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAG
GCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGA
TGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATT
TATTCAATATGTACCGCAACTTTTGTTCTAACAAGGGTTGAAAGTTGCAAATTAACGAAATGATCCCATCCAGTGTCGGG
TTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACA
ACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGG
TTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGG
ACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAA
CCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTT
TGCGTAAAAGTCAGGTTGGGTCAGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGAC
CACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGC
AGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAA

Upstream 100 bases:

>100_bases
GTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACC
GTTCTTTAAACGAACTGTAG

Downstream 100 bases:

>100_bases
TGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTA
TTAGAAAAGTTTGGTCTGCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDE
AKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKGWKLQINEMIPSSVG
LNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNR
TESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTD
HRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS

Sequences:

>Translated_359_residues
MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDE
AKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKG*KLQINEMIPSSVG
LNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNR
TESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTD
HRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS
>Mature_359_residues
MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDE
AKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKG*KLQINEMIPSSVG
LNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNR
TESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTD
HRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=46.1805555555556, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=35.1274787535411, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI166795305, Length=192, Percent_Identity=44.7916666666667, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1787462, Length=325, Percent_Identity=41.8461538461538, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=248, Percent_Identity=40.3225806451613, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6321295, Length=362, Percent_Identity=41.4364640883978, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI19921226, Length=316, Percent_Identity=38.6075949367089, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40608; Mature: 40608

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLID
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
QALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCCEEEEEEC
AAGGDESCIFLADLFNMYRNFCSNKGKLQINEMIPSSVGLNFVSFEVNGVDVFAKLKFES
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCHHHCCEEEEEEECCEEEEEEEEHHH
GVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRT
HHHHHHHCCCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHEEEEEEECCCCCCCCCCCC
ESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCC
GDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS
CCHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEECCHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLID
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
QALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCCEEEEEEC
AAGGDESCIFLADLFNMYRNFCSNKGKLQINEMIPSSVGLNFVSFEVNGVDVFAKLKFES
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEEHHHCCHHHCCEEEEEEECCEEEEEEEEHHH
GVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRT
HHHHHHHCCCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHEEEEEEECCCCCCCCCCCC
ESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCC
GDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS
CCHHHHHHCCCCCCCCCCCEEEEEEEECCCEEEECCHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]