Click here to switch to the map view.

The map label for this gene is yfcB [C]

Identifier: 13508101

GI number: 13508101

Start: 431707

End: 433068

Strand: Direct

Name: yfcB [C]

Synonym: MPN362

Alternate gene names: 13508101

Gene position: 431707-433068 (Clockwise)

Preceding gene: 13508100

Following gene: 13508102

Centisome position: 52.88

GC content: 39.43

Gene sequence:

>1362_bases
ATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCT
ATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGAAAGCACGCTAAAACAATTACTGACTTTGACGAAC
AGGGAATTGTTAATGCTTTAAAAGCTTACTTTGTCGATCAACTACCCTTACCCTATATTACCGGGAGTGTTAAGCTAGGT
TCATTAACTTTTAAAACTCAACCTGGCGTCTTTATTCCTCGTGCCGACAGTTTAGCGCTTTTAAAGGTAGTCAAAGCACA
AAACCTTAAAACCGCGGTTGACCTTTGCTGTGGAAGCGGTACGTTAGCAATTGCGCTAAAAAAGCGTTTTCCTCACTTAA
ATGTTTATGGTAGTGACCTCAACCCCCAAGCATTGCAGTTAGCGGCGCAAAATGCCCGTTTGAATATGGTTGAAGTGCAG
TGAATTGAAGCTGATTTTTTAGCTGCTCTAGCACAGGTAAACACACCTATTGATCTCATTATTACCAATCCACCCTATTT
AAACGAAAGTCAGTTAGATCAAACTTTAAACCACGAACCGCGCAACAGTTTAGTGGCTGATGGTAACGGAATTTTGTTTT
ACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAA
GAGTTTTTAGCCTTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGA
CAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTA
TCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAG
CACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGACC
AGGTGCTTACACCTTTATTGTAGATGGACAGAGCTATCGTCTACCCAACAGCCCACAGCTGTTAAAGCTCTTAAAAACGG
TTGGTTTAATTTACTGTACCAGTGCTAATCAAGCTAAACAAAAACCATTTGGCAAGTTAAGCGCTTACCAGAACGATCCT
TACTGAGTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGA
TACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACT
AA

Upstream 100 bases:

>100_bases
TCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAA
CTAGAGAACTTTTTCAGTTA

Downstream 100 bases:

>100_bases
AAGTTTCAAATTTGACTGCTAAAAAGCACTATTAACGGATTATTGGAGCTATCCAACTTATTTTTAACTTTTAAAAATAA
AGAATTTGCACCATTGCTGT

Product: HemK family methylase

Products: S-adenosyl-L-homocysteine; DNA methylaminopurine [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTALWKHAKTITDFDEQGIVNALKAYFVDQLPLPYITGSVKLG
SLTFKTQPGVFIPRADSLALLKVVKAQNLKTAVDLCCGSGTLAIALKKRFPHLNVYGSDLNPQALQLAAQNARLNMVEVQ
WIEADFLAALAQVNTPIDLIITNPPYLNESQLDQTLNHEPRNSLVADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKK
EFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNK
HYVQFLAPSQINQLPKQLQKLAKLFWPGAYTFIVDGQSYRLPNSPQLLKLLKTVGLIYCTSANQAKQKPFGKLSAYQNDP
YWVQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH

Sequences:

>Translated_453_residues
MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTAL*KHAKTITDFDEQGIVNALKAYFVDQLPLPYITGSVKLG
SLTFKTQPGVFIPRADSLALLKVVKAQNLKTAVDLCCGSGTLAIALKKRFPHLNVYGSDLNPQALQLAAQNARLNMVEVQ
*IEADFLAALAQVNTPIDLIITNPPYLNESQLDQTLNHEPRNSLVADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKK
EFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNK
HYVQFLAPSQINQLPKQLQKLAKLF*PGAYTFIVDGQSYRLPNSPQLLKLLKTVGLIYCTSANQAKQKPFGKLSAYQNDP
Y*VQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH
>Mature_453_residues
MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTAL*KHAKTITDFDEQGIVNALKAYFVDQLPLPYITGSVKLG
SLTFKTQPGVFIPRADSLALLKVVKAQNLKTAVDLCCGSGTLAIALKKRFPHLNVYGSDLNPQALQLAAQNARLNMVEVQ
*IEADFLAALAQVNTPIDLIITNPPYLNESQLDQTLNHEPRNSLVADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKK
EFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNK
HYVQFLAPSQINQLPKQLQKLAKLF*PGAYTFIVDGQSYRLPNSPQLLKLLKTVGLIYCTSANQAKQKPFGKLSAYQNDP
Y*VQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH

Specific function: Unknown

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI87082085, Length=222, Percent_Identity=27.4774774774775, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787463, Length=162, Percent_Identity=30.8641975308642, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR007848
- InterPro:   IPR006070 [H]

Pfam domain/function: PF05175 MTS; PF01300 Sua5_yciO_yrdC [H]

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 50442; Mature: 50442

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00092 N6_MTASE ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTALKHAKTITDFDEQGIVNALK
CCHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AYFVDQLPLPYITGSVKLGSLTFKTQPGVFIPRADSLALLKVVKAQNLKTAVDLCCGSGT
HHHHHCCCCCEEECCEEEEEEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCE
LAIALKKRFPHLNVYGSDLNPQALQLAAQNARLNMVEVQIEADFLAALAQVNTPIDLIIT
EEEEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCEEEEEC
NPPYLNESQLDQTLNHEPRNSLVADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKKEF
CCCCCCHHHHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
LALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGL
HHHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCHHH
MSYCQQDLDHIKQRDPNKHYVQFLAPSQINQLPKQLQKLAKLFPGAYTFIVDGQSYRLPN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SPQLLKLLKTVGLIYCTSANQAKQKPFGKLSAYQNDPYVQQNCFIVQNSFKSNNEPSLIY
CHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEE
NLDTKQIVRGSSTQLQRFQALLAKHKLRH
ECCHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTALKHAKTITDFDEQGIVNALK
CCHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AYFVDQLPLPYITGSVKLGSLTFKTQPGVFIPRADSLALLKVVKAQNLKTAVDLCCGSGT
HHHHHCCCCCEEECCEEEEEEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCE
LAIALKKRFPHLNVYGSDLNPQALQLAAQNARLNMVEVQIEADFLAALAQVNTPIDLIIT
EEEEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCEEEEEC
NPPYLNESQLDQTLNHEPRNSLVADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKKEF
CCCCCCHHHHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
LALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGL
HHHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCHHH
MSYCQQDLDHIKQRDPNKHYVQFLAPSQINQLPKQLQKLAKLFPGAYTFIVDGQSYRLPN
HHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SPQLLKLLKTVGLIYCTSANQAKQKPFGKLSAYQNDPYVQQNCFIVQNSFKSNNEPSLIY
CHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEE
NLDTKQIVRGSSTQLQRFQALLAKHKLRH
ECCHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; DNA adenine; DNA [C]

Specific reaction: S-Adenosyl-L-methionine + DNA adenine =S-adenosyl-L-homocysteine + DNA methylaminopurine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]